FB2025_01 , released February 20, 2025
Allele: Dmel\Ras85DNIG.9375R
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General Information
Symbol
Dmel\Ras85DNIG.9375R
Species
D. melanogaster
Name
FlyBase ID
FBal0257034
Feature type
allele
Associated gene
Dmel\Ras85D
Associated Insertion(s)
Carried in Construct
P{NIG.9375R}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Buchon et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Buchon et al., 2010)
Mutagen
in vitro construct
(Buchon et al., 2010)
Progenitor genotype
Carried in construct
P{NIG.9375R}
Cytology
Description

UASt regulatory sequences drive expression of a Ras85D RNAi construct.

(Buchon et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-9375R
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Ras85DNIG.9375R in intestinal stem cells (ISCs) driven by Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B does not significantly affect the number of ISCs in the gut. However, in sharp contrast to wild-type flies, Erwinia carotovora carotovora 15 (Ecc15) infection does not increase the number of intestinal mitotic cells.

      (Buchon et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Ras85DNIG.9375R blocks the epithelium renewal induced by upd3Scer\UAS.cMa-expression under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B.

      Co-expression of Ras85DNIG.9375R blocks the extra ISC proliferation induced by domeScer\UAS.T:Avic\GFP-EGFP-expression under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B.

      (Buchon et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ras85DNIG.9375R
      Name Synonyms
      Secondary FlyBase IDs
        References (5)