FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Vps16B1
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General Information
Symbol
Dmel\Vps16B1
Species
D. melanogaster
Name
FlyBase ID
FBal0258124
Feature type
allele
Associated gene
Dmel\Vps16B
Associated Insertion(s)
TI{TI}Vps16B1
Carried in Construct
Also Known As
fob1
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ends-out gene targeting
(Akbar et al., 2011)
Progenitor genotype
Associated Insertion(s)
TI{TI}Vps16B1
Cytology
Description

The entire Vps16B open reading frame has been replaced by a w+mW.hs marker.

(Akbar et al., 2011)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  ARC syndrome
      CEA
      (Akbar et al., 2011)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Vps16B1 homozygous adults show significant decrease in lifespan upon infection with heat-killed E.coli bacteria, as compared to infected controls.

      (Akbar et al., 2016)

      Vps16B1 flies exhibit a profound defect in phagosome maturation, and as a consequence are sensitive to infections with normally non-pathogenic microbes. Vps16B1mutants are strongly susceptible to infections with Escherichia coli (that are not normally pathogenic to flies).

      Pathogenic Enterococcus faecalis microbes kill Vps16B1 flies only slightly faster than wild-type.

      Vps16B1 hemocytes do not exhibit a defect in initial phagocytic uptake of bacteria. After 45 minutes however, Vps16B1 mutant hemocytes are full of bacteria and do not seem able to digest them. The bacterial burden remains elevated, as opposed to the efficient clearance of the majority of bacteria in wild-type flies after just 1 day.

      Vps16B1 phagosomes fail to acidify.

      Eyes of two-day old Vps16B1 mutants exhibit normal ommatidial organization.

      Starvation-induced autophagy, which requires fusion with lysosomes, is normal in Vps16B1 larvae.

      Vps16B1 flies exhibit normal lysosomal function.

      Vps16B1 flies do not show any morphological defects.

      (Akbar et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Vps16B1 is rescued by Vps16B+t

      (Akbar et al., 2016)

      Vps16B1 is rescued by Vps16BUAS.Tag:MYC/Scer\GAL4srp.Hemo

      (Akbar et al., 2011)

      Vps16B1 is rescued by Scer\GAL4da.G32/Vps16BUAS.Tag:MYC

      (Akbar et al., 2011)

      Vps16B1 is rescued by Vps16B+t

      (Akbar et al., 2011)
      Comments

      The survival of Vps16B1 flies is significantly improved through expression of Vps16BScer\UAS.T:Hsap\MYC in hemocytes under the control of Scer\GAL4srp.Hemo or ubiquitously under the control of Scer\GAL4da.G32.

      Expression of Vps16B+t significantly improves survival of Vps16B1 flies.

      (Akbar et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Vps16B1
      fob1
      (Akbar et al., 2016, Takáts et al., 2014, Akbar et al., 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)