βTub85D promoter sequences drive expression of a deleted form of aux (lacking amino acid residues 768-1049, which includes the clathrin-binding motif) which is tagged at the C-terminal end with mRFP(Unk).
auxΔC.βTub85D.mRFP(Unk) fails to rescue auxI670K/auxG257E
Expression of auxΔC.βTub85D.T:Disc\RFP-mRFP in auxI670K/auxG257E mutants fails to rescue male sterility.