FlyBase Allele Report: Dmel\aux[I670K]

aux^I670K/aux^L78H adults exhibit rough eyes, with extra photoreceptor cells and occasional extra bristles on the notum, sternopleurum, and scutellum.

aux^I670K/aux^L78H mutant males, when mated to wild-type females, do not produce any embryos that hatch. Spermatids do develop in the testis, however the seminal vesicles are empty in adult male flies. The morphology of the testis hub cells appear normal in these animals. The number of germ cells is comparable to wild-type testes. Spermatid individualisation is disrupted in mutants, and migration of the investment cone along spermatid cysts does not occur normally.

aux^I670K/aux^G257E adults exhibit rough eyes, with extra photoreceptor cells and occasional extra bristles on the notum, sternopleurum, and scutellum.

aux^I670K/aux^G257E mutant males, when mated to wild-type females, do not produce any embryos that hatch. Spermatids do develop in the testis, however the seminal vesicles are empty in adult male flies. The morphology of the testis hub cells appear normal in these animals. A slightly reduced number of germ cells is observed compared to wild-type testes. Cytokinesis is mostly normal in these animals. In 5% of spermatids examined, an enlarged mitochondrial derivative associated with two nuclei is observed. Spermatid individualisation is disrupted in mutants, and migration of the investment cone along spermatid cysts does not occur normally.

(Zhou et al., 2011)

Homozygous aux^I670K flies can survive until adulthood.

Mutants homozygous for aux^I670K exhibit several morphological defects, including rough eyes, extra bristles, missing wing veins, and male and female sterility. The eyes of aux^I670K mutants are grossly disorganised, with patches of brown necrotic tissues on the surface. This rough eye phenotype shows temperature dependence, as the eye roughness is significantly milder for mutants raised at 18^oC than those grown at 25^oC. At a higher temperature, such as 29</up>o</up>C, the homozygous mutant state is completely lethal.

aux^I670K mutants exhibit supernumerary vibrissae, and frequently, extra anterior sternopleural bristles. Occasionally, extra bristles are also detected on the notum or scutellum of mutant animals. At low frequency, some mutant animals exhibit wings with incompletely formed posterior crossveins, and absent wing vein material at the posterior wing vein margin. The penetrance and severity of these phenotypes are more pronounced in mutant animals heteroallelic for aux^W328X/aux^I670K and aux^W1150X/aux^I670K raised at 18^oC.

Sections of aux^I670K mutant retina exhibit disrupted ommatidia. Supernumerary photoreceptor cells are detected in 39.67% of aux^I670K mutant clusters. These extra photoreceptors are either outer (cells with large rhabdomeres; 21.42%) or inner (cells with small rhabdomeres; 18.25%), suggesting that aux^I670K is not affecting the determination of a particular photoreceptor cell fate.

elav-positive cell clusters are clearly disorganised in aux^I670K tissues, with supernumerary elav-positive cells in some of the clusters.

Embryos derived from the mating of aux^I670K homozygous females with wild-type males exhibit highly organised central and peripheral nervous systems with characteristic numbers of neuro-positive cells. In contrast, embryos maternally deficient but zygotically heterozygous for aux^I670K exhibit mild hypertrophy in the ventral nerve cord, disorganisation and slight reduction in the peripheral nervous system, and abnormal body morphology.

Homozygous mutant aux^I670K eye discs exhibit excessive elav-positive cells.

(Hagedorn et al., 2006)

External Data

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Interactions

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Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

aux^I670K has eye phenotype, enhanceable by Scer\GAL4^Act5C.PI/Clc^UAS.EGFP

(Hagedorn et al., 2006)

aux^I670K has wing phenotype, enhanceable by Scer\GAL4^Act5C.PI/Clc^UAS.EGFP

(Hagedorn et al., 2006)

aux^I670K has wing vein phenotype, enhanceable by Scer\GAL4^Act5C.PI/Clc^UAS.EGFP

(Hagedorn et al., 2006)

aux^I670K has wing vein | ectopic phenotype, enhanceable by Scer\GAL4^Act5C.PI/Clc^UAS.EGFP

(Hagedorn et al., 2006)

Enhancer of

Statement

Reference

aux^I670K/aux[+] is an enhancer of posterior wing margin phenotype of N^264-39

(Hagedorn et al., 2006)

aux^I670K/aux[+], Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsc70-4^K71S.UAS, Scer\GAL4^GMR.PF

(Hagedorn et al., 2006)

aux^I670K/aux[+] is an enhancer of eye phenotype of N^UAS.cMa, Scer\GAL4^GMR.PF

(Hagedorn et al., 2006)

NOT Enhancer of

Statement

Reference

aux^I670K/aux[+] is a non-enhancer of eye phenotype of Egfr^UAS.cBa, Scer\GAL4^GMR.PF

(Hagedorn et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Hsc70-4^{K71S.Scer\UAS} under the control of Scer\GAL4^GMR.PF in a aux^I670K/+ background generates a severe rough eye phenotype, more severe than expression of Hsc70-4^{K71S.Scer\UAS} under the control of Scer\GAL4^GMR.PF in a wild-type background.

The expression of Clc^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Act5C.PI greatly reduces the viability of aux^I670K mutants. In rare escapers, the eyes are rougher, and there is dramatic enhancement of the wing phenotypes, including severe notching, wing vein thickening, and ectopic vein formation.

Homozygous mutant aux^I670K eye discs that also express N^ICN.Scer\UAS (under the control of Scer\GAL4^GMR.PF) exhibit a reduction in the number of elav-positive cells, indicating that N is epistatic to aux.

A heterozygous aux^I670K background increases the severity of both the penetrance and the phenotype of N^264-39 heterozygous mutants.

Although animals heterozygous for aux^I670K are normal, this background strongly reduces the eye size and enhances the rough eye of N^Scer\UAS.cMa;Scer\GAL4^GMR.PF animals.

(Hagedorn et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

aux^I670K is rescued by Scer\GAL4^Act5C.PI/aux^UAS.cHa

(Hagedorn et al., 2006)

Partially rescued by

aux^I670K/aux^G257E is partially rescued by aux^{ΔK.βTub85D.mRFP(Unk)}

(Zhou et al., 2011)

aux^I670K/aux^G257E is partially rescued by aux^{CJ.βTub85D.mRFP(Unk)}

(Zhou et al., 2011)

aux^I670K/aux^G257E is partially rescued by aux^{FL.βTub85D.mRFP(Unk)}

(Zhou et al., 2011)

Not rescued by

aux^I670K/aux^G257E is not rescued by aux^{ΔC.βTub85D.mRFP(Unk)}

(Zhou et al., 2011)

aux^I670K/aux^G257E is not rescued by aux^{ΔJ.βTub85D.mRFP(Unk)}

(Zhou et al., 2011)

Comments

Expression of aux^{FL.βTub85D.T:Disc\RFP-mRFP} in aux^I670K/aux^G257E mutants rescues male sterility. The rough eye and supernumerary bristle phenotypes are not rescued.

Expression of aux^{ΔK.βTub85D.T:Disc\RFP-mRFP} in aux^I670K/aux^G257E mutants rescues male sterility.

Expression of aux^{CJ.βTub85D.T:Disc\RFP-mRFP} in aux^I670K/aux^G257E mutants rescues male sterility.

Expression of aux^{ΔJ.βTub85D.T:Disc\RFP-mRFP} in aux^I670K/aux^G257E mutants fails to rescue male sterility.

Expression of aux^{ΔC.βTub85D.T:Disc\RFP-mRFP} in aux^I670K/aux^G257E mutants fails to rescue male sterility.

(Zhou et al., 2011)

Expression of aux^Scer\UAS.cHa (under the control of Scer\GAL4^Act5C.PI) completely rescues the rough eye phenotype of aux^I670K.

(Hagedorn et al., 2006)

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Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Aux^I670K

(Inoshita et al., 2022)

aux^I670K

(Cheli et al., 2010)

dAux^I670K

(Zhou et al., 2011, Kandachar et al., 2008, Hagedorn et al., 2006)

daux^I670K

(Zhang et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (6)

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