βTub85D promoter sequences drive expression of amino acid residues 1-998 of aux (this lacks the J-domain) which is tagged at the C-terminal end with mRFP(Unk).
auxΔJ.βTub85D.mRFP(Unk) fails to rescue auxI670K/auxG257E
Expression of auxΔJ.βTub85D.T:Disc\RFP-mRFP in auxI670K/auxG257E mutants fails to rescue male sterility.