FlyBase Allele Report: Dmel\aux[G257E]

General Information

Symbol

Dmel\aux^G257E

Species

D. melanogaster

Name

FlyBase ID

FBal0240440

Feature type

allele

Associated gene

Dmel\aux

Associated Insertion(s)

Carried in Construct

Also Known As

dAux^G257E

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Kandachar et al., 2008)

Progenitor genotype

Cytology

Description

Amino acid replacement: G257E.

(Kandachar et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:4,222,481..4,222,481 [+]

Nucleotide change:

G4222481A

Amino acid change:

G257E | aux-PA; G257E | aux-PB; G257E | aux-PC; G249E | aux-PD

Reported amino acid change:

G257E

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Kandachar et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male sterile (with aux^I670K)

(Zhou et al., 2011)

visible

(Cheli et al., 2010)

Phenotype Manifest In

adult glial cell | somatic clone

(Zhang et al., 2023)

autophagosome | adult stage | increased number | somatic clone

(Zhang et al., 2023)

cystic bulge (with aux^I670K)

(Zhou et al., 2011)

eye

(Cheli et al., 2010)

eye (with aux^I670K)

(Zhou et al., 2011)

eye photoreceptor cell | increased number (with aux^I670K)

(Zhou et al., 2011)

individualization stage spermatid (with aux^I670K)

(Zhou et al., 2011)

male germline stem cell (with aux^I670K)

(Zhou et al., 2011)

scutellar bristle | increased number (with aux^I670K)

(Zhou et al., 2011)

sternopleural bristle | increased number (with aux^I670K)

(Zhou et al., 2011)

Detailed Description

Statement

Reference

aux^I670K/aux^G257E adults exhibit rough eyes, with extra photoreceptor cells and occasional extra bristles on the notum, sternopleurum, and scutellum.

aux^I670K/aux^G257E mutant males, when mated to wild-type females, do not produce any embryos that hatch. Spermatids do develop in the testis, however the seminal vesicles are empty in adult male flies. The morphology of the testis hub cells appear normal in these animals. A slightly reduced number of germ cells is observed compared to wild-type testes. Cytokinesis is mostly normal in these animals. In 5% of spermatids examined, an enlarged mitochondrial derivative associated with two nuclei is observed. Spermatid individualisation is disrupted in mutants, and migration of the investment cone along spermatid cysts does not occur normally.

(Zhou et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

aux^I670K/aux^G257E is partially rescued by aux^{ΔK.βTub85D.mRFP(Unk)}

(Zhou et al., 2011)

aux^I670K/aux^G257E is partially rescued by aux^{CJ.βTub85D.mRFP(Unk)}

(Zhou et al., 2011)

aux^I670K/aux^G257E is partially rescued by aux^{FL.βTub85D.mRFP(Unk)}

(Zhou et al., 2011)

Not rescued by

aux^I670K/aux^G257E is not rescued by aux^{ΔC.βTub85D.mRFP(Unk)}

(Zhou et al., 2011)

aux^I670K/aux^G257E is not rescued by aux^{ΔJ.βTub85D.mRFP(Unk)}

(Zhou et al., 2011)

Comments

Expression of aux^{FL.βTub85D.T:Disc\RFP-mRFP} in aux^I670K/aux^G257E mutants rescues male sterility. The rough eye and supernumerary bristle phenotypes are not rescued.

Expression of aux^{ΔK.βTub85D.T:Disc\RFP-mRFP} in aux^I670K/aux^G257E mutants rescues male sterility.

Expression of aux^{CJ.βTub85D.T:Disc\RFP-mRFP} in aux^I670K/aux^G257E mutants rescues male sterility.

Expression of aux^{ΔJ.βTub85D.T:Disc\RFP-mRFP} in aux^I670K/aux^G257E mutants fails to rescue male sterility.

Expression of aux^{ΔC.βTub85D.T:Disc\RFP-mRFP} in aux^I670K/aux^G257E mutants fails to rescue male sterility.

(Zhou et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

aux^G257E

(Cheli et al., 2010)

dAux^G257E

(Zhou et al., 2011, Kandachar et al., 2008)

daux^G257E

(Zhang et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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