FlyBase Allele Report: Dmel\stan[E86]

General Information

Symbol

Dmel\stan^E86

Species

D. melanogaster

Name

FlyBase ID

FBal0258385

Feature type

allele

Associated gene

Dmel\stan

Associated Insertion(s)

Carried in Construct

Also Known As

fmi^E86

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele

Mutagen

Nature of the Allele

Allele class

hypomorphic allele

(Hakeda-Suzuki et al., 2011)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: D1297N.

(Hakeda-Suzuki et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:10,710,488..10,710,488 [+]

Nucleotide change:

G10710488A

Amino acid change:

Reported amino acid change:

D1297N

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Hakeda-Suzuki et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage (with stan^E59)

(Matsubara et al., 2011)

partially lethal - majority die (with stan^E59)

(Hakeda-Suzuki et al., 2011)

Phenotype Manifest In

dendrite | third instar larval stage (with stan^E59)

(Matsubara et al., 2011)

larval dorsal multidendritic neuron ddaC | third instar larval stage (with stan^E59)

(Matsubara et al., 2011)

larval multidendritic class IV neuron

(Hakeda-Suzuki et al., 2011)

photoreceptor cell R8 | somatic clone

(Hakeda-Suzuki et al., 2011)

Detailed Description

Statement

Reference

Mosaic animals containing homozygous R8 photoreceptor axons in an otherwise wild-type background only occasionally have defects in R8 axon targeting (the axons occasionally innervate neighbouring medulla columns and make abnormal contacts with other R8 photoreceptor axons).

Class IV dendrites cross the midline in 15.3% of cases in homozygous embryos.

(Hakeda-Suzuki et al., 2011)

stan^E86/stan^E59 transheterozygotes survive until wandering third larval instar stages and even up to the adult stage.

The branches of the ddaC class IV neurons show a four-fold increase in the crossing index within the dorsal region of the arbor in stan^E86/stan^E59 third instar larvae compared to wild type, although the cumulative branch length of the dendrites is unchanged in the mutant larvae. The class IV neurons also show occasional axon misrouting and stalling of growth cones.

(Matsubara et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

stan^E86/stan^E59 has partially lethal - majority die phenotype, enhanceable by gogo^H1675

(Hakeda-Suzuki et al., 2011)

stan^E86/stan^E59 has partially lethal - majority die phenotype, enhanceable by gogo^D869

(Hakeda-Suzuki et al., 2011)

stan^E86 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by gogo[+]/gogo^D869

(Hakeda-Suzuki et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

stan^E86 has larval multidendritic class IV neuron phenotype, enhanceable by gogo[+]/gogo^D869

(Hakeda-Suzuki et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

gogo^H1675/+ or gogo^D869/+ substantially increases the incidence of R8 photoreceptor axon defects in mosaic animals containing homozygous stan^E86 R8 photoreceptor axons.

The dorsal-midline crossing phenotype seen in class IV neurons of stan^E86 embryos is enhanced by gogo^D869/+ (midline crossing is seen in 29.6% of cases).

(Hakeda-Suzuki et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

stan^E86/stan^E59 is rescued by stan^UAS.TTV/Scer\GAL4⁴⁷⁷

(Matsubara et al., 2011)

stan^E86/stan^E59 is rescued by stan^UAS.AEY/Scer\GAL4⁴⁷⁷

(Matsubara et al., 2011)

Not rescued by

stan^E86/stan^E59 is not rescued by stan^{ΔJM-A.UAS.TTV}/Scer\GAL4⁴⁷⁷

(Matsubara et al., 2011)

Comments

Expression of stan^Scer\UAS.TTV or of stan^Scer\UAS.AEY under the control of Scer\GAL4⁴⁷⁷ rescues the increased crossing that is seen in the dorsal dendritic arbor of the ddaC class IV neurons in stan^E86/stan^E59 third instar larvae.

Expression of stan^{ΔJM-A.Scer\UAS.TTV} under the control of Scer\GAL4⁴⁷⁷ does not rescue the increased crossing that is seen in the dorsal dendritic arbor of the ddaC class IV neurons in stan^E86/stan^E59 third instar larvae.

(Matsubara et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

fmi^E86

(Hakeda-Suzuki et al., 2011, Hakeda-Suzuki et al., 2011, Matsubara et al., 2011)

stan^E86

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close