FlyBase Allele Report: Dmel\α-Spec[KK101541]

General Information

Symbol

Dmel\α-Spec^KK101541

Species

D. melanogaster

Name

FlyBase ID

FBal0258977

Feature type

allele

Associated gene

Dmel\α-Spec

Associated Insertion(s)

Carried in Construct

P{KK101541}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK101541}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK101541

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Keller et al., 2011)

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav.PU

(Brace et al., 2014)

Phenotype Manifest In

embryonic/larval neuromuscular junction, with Scer\GAL4^elav-C155

(Keller et al., 2011)

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^elav.PU

(Brace et al., 2014)

epithelium, with Scer\GAL4^NP1624

(Berns et al., 2014)

female germline cyst, with Scer\GAL4^NP1624

(Berns et al., 2014)

Detailed Description

Statement

Reference

Scer\GAL4^tj-NP1624-mediated expression results in the transformation of the normal germline cyst epithelial monolayer into two or more cell layers.

(Berns et al., 2014)

Expression of α-Spec^KK101541 under the control of Scer\GAL4^elav.PU results in synaptic retractions in 47% of muscle 6/7 synapses.

(Brace et al., 2014)

Expression of α-Spec^KK101541 under the control of Scer\GAL4^elav-C155 results in synaptic degeneration.

(Keller et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

α-Spec^KK101541, Scer\GAL4^elav-C155 has abnormal neuroanatomy phenotype, enhanceable by bsk^K53R.UAS, Scer\GAL4^elav-C155

(Keller et al., 2011)

Suppressed by

Statement

Reference

α-Spec^KK101541, Scer\GAL4^elav.PU has abnormal neuroanatomy | larval stage phenotype, suppressible by SkpA^GD65

(Brace et al., 2014)

α-Spec^KK101541, Scer\GAL4^elav-C155 has abnormal neuroanatomy phenotype, suppressible by Miro^B682

(Keller et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

α-Spec^KK101541, Scer\GAL4^elav-C155 has embryonic/larval neuromuscular junction phenotype, enhanceable by bsk^K53R.UAS, Scer\GAL4^elav-C155

(Keller et al., 2011)

Suppressed by

Statement

Reference

α-Spec^KK101541, Scer\GAL4^elav.PU has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by SkpA^GD65

(Brace et al., 2014)

α-Spec^KK101541, Scer\GAL4^elav-C155 has embryonic/larval neuromuscular junction phenotype, suppressible by Miro^B682

(Keller et al., 2011)

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/α-Spec^KK101541 is a non-enhancer of mitochondrion phenotype of Miro^B682

(Keller et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The synaptic retraction phenotype seen at the neuromuscular junction in larvae expressing α-Spec^KK101541 under the control of Scer\GAL4^elav.PU is suppressed by SkpA^GD65.

(Brace et al., 2014)

Expression of bsk^{K53R.Scer\UAS} enhances the synaptic degeneration phenotype seen when α-Spec^KK101541 is expressed under the control of Scer\GAL4^elav-C155.

Homozygous Miro^B682 suppresses the neuromuscular degeneration seen when α-Spec^KK101541 is expressed under the control of Scer\GAL4^elav-C155. The decrease in the number of mitochondria seen in Miro^B682 mutants is also seen in these larvae.

(Keller et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v110417

P{KK101541}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

α-Spec^KK101541

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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