Relatively few mutant sperm from homozygous lobolobo males are observed to enter the females storage organs by 30 minutes after mating. However, sperm insemination into the uterus is unaffected. The same phenotype is observed for sperm from lobolobo/Df(3R)Exel6201 or lobolobo/loboMB00722males.
98% of lobolobo/loboMB00722 mutant sperm exit the converging tubule with head-leading orientation, as wild type.
In contrast to wild-type sperm, sperm from lobolobo/loboMB00722 males show increased frequencies of head-leading orientation during entry into the proximal seminal receptacle (PSR) and excessive flagellar folding in the PSR lumen. Mutant sperm also show excessive folding into tangles in the PSR. At later time points (1-4 hours after mating), the phenotype is more severe with mostly tangled flagella in the PSR lumen, and the number of mutant sperm that entering the distal seminal receptacle reduced by 100-fold.
In contrast to wild type, the number of lobolobo/loboMB00722 sperm that enter the distal seminal receptacle (DSR) vary greatly on an individual basis and the average number of sperm stored per female per mating is only 4. Only 3.9 F1 progeny are produced per female from matings to these mutant males.
Sperm from lobolobo/loboMB00722 males show head-leading orientation when they exit the proximal seminal receptacle, as wild type.