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General Information
Symbol
Dmel\lobolobo
Species
D. melanogaster
Name
FlyBase ID
FBal0261062
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    deletion
    Comment:

    7 bp deletion of (CTGCACT) in lobo reported to produce a 567 aa truncated polypeptide.

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    A 7 bp deletion (CTGCACT) in exon 6 of lobo, which produces a 567 amino acid truncated polypeptide.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Relatively few mutant sperm from homozygous lobolobo males are observed to enter the females storage organs by 30 minutes after mating. However, sperm insemination into the uterus is unaffected. The same phenotype is observed for sperm from lobolobo/Df(3R)Exel6201 or lobolobo/loboMB00722males.

    98% of lobolobo/loboMB00722 mutant sperm exit the converging tubule with head-leading orientation, as wild type.

    In contrast to wild-type sperm, sperm from lobolobo/loboMB00722 males show increased frequencies of head-leading orientation during entry into the proximal seminal receptacle (PSR) and excessive flagellar folding in the PSR lumen. Mutant sperm also show excessive folding into tangles in the PSR. At later time points (1-4 hours after mating), the phenotype is more severe with mostly tangled flagella in the PSR lumen, and the number of mutant sperm that entering the distal seminal receptacle reduced by 100-fold.

    In contrast to wild type, the number of lobolobo/loboMB00722 sperm that enter the distal seminal receptacle (DSR) vary greatly on an individual basis and the average number of sperm stored per female per mating is only 4. Only 3.9 F1 progeny are produced per female from matings to these mutant males.

    Sperm from lobolobo/loboMB00722 males show head-leading orientation when they exit the proximal seminal receptacle, as wild type.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Sperm from lobolobo/loboMB00722; Pkd2ko67 / Pkd2MB06703 double mutants are motile, are produced in large numbers and can enter the uterus as normal. However, in contrast to wild type, the number of sperm that enter the distal seminal receptacle (DSR) vary greatly on an individual basis and the average number of sperm stored per female per mating is only 2.4. Only 2.9 F1 progeny are produced per female from matings to these double-mutant males.

    Stored sperm from lobolobo/loboMB00722; Pkd2ko67 / Pkd2MB06703 double mutant males can exit the distal seminal receptacle and fertilize the egg.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer

    Separable from: kal-1[d01966]

    The lobolobo male semi-sterile mutation was originally present on a chromosome with 2 other known mutations. It was separated from the kal-1d01966 mutation (associated with the P{XP}kal-1d01966 insertion) and a second male sterile mutation located at the tip of 3R.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    CG34110lobo
    lobolobo
    Name Synonyms
    Secondary FlyBase IDs
      References (1)