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General Information
Symbol
Mmus\Itpr1IP3-sponge.m49.UAS
Species
M. musculus
Name
FlyBase ID
FBal0261174
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inositol 1, 4, 5-trisphosphate (IP[[3]]) "sponge" which retains the ability to bind IP[[3]] without exerting any physiological activity, thus titrating cellular IP[[3]]. The sponge sequence consists of a 380 amino acid peptide derived from the ligand binding site of the Mmus\Itpr1 gene which has been mutated to carry the amino acid replacement R441Q.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Animals expressing Mmus\Itpr1IP3-sponge.m49.Scer\UAS under the control of Scer\GAL4fkh.PU show inhibition of autophagy in the salivary glands 14 hours after pupariation and salivary gland fragments persist in these animals 24 hours after puparium (no residual salivary gland material is present in wild-type animals at this stage).

Expression of Mmus\Itpr1IP3-sponge.m49.Scer\UAS under the control of Scer\GAL4Gr5a.853 changes the sensitivity of flies to sugars, with expression resulting in less of a preference towards trehalose and an increase in consumption of sucrose.

Flies expressing Mmus\Itpr1IP3-sponge.m49.Scer\UAS under the control of Scer\GAL4Gr5a.853 exhibit a decrease in trehalose consumption at a variety of trehalose concentration.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The inhibition of autophagy which is seen in the salivary glands 14 hours after pupariation in animals expressing Mmus\Itpr1IP3-sponge.m49.Scer\UAS under the control of Scer\GAL4fkh.PU is still seen if the animals are also mutant for IP3K2Δ1.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Mmus\Itpr1IP3-sponge.m49.Scer\UAS
Mmus\Itpr1IP3-sponge.m49.UAS
Mmus\Itpr1R441Q.Scer\UAS
Name Synonyms
Secondary FlyBase IDs
    References (2)