FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Mmus\Itpr1IP3-sponge.m49.UAS
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General Information
Symbol
Mmus\Itpr1IP3-sponge.m49.UAS
Species
M. musculus
Name
FlyBase ID
FBal0261174
Feature type
allele
Associated gene
Mmus\Itpr1
Associated Insertion(s)
Carried in Construct
P{UAS-IP3-sponge.m49}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Usui-Aoki et al., 2005)
Progenitor genotype
Carried in construct
P{UAS-IP3-sponge.m49}
Cytology
Description

UASt regulatory sequences drive expression of an inositol 1, 4, 5-trisphosphate (IP[[3]]) "sponge" which retains the ability to bind IP[[3]] without exerting any physiological activity, thus titrating cellular IP[[3]]. The sponge sequence consists of a 380 amino acid peptide derived from the ligand binding site of the Mmus\Itpr1 gene which has been mutated to carry the amino acid replacement R441Q.

(Usui-Aoki et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Mmus\Itpr1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Animals expressing Mmus\Itpr1IP3-sponge.m49.Scer\UAS under the control of Scer\GAL4fkh.PU show inhibition of autophagy in the salivary glands 14 hours after pupariation and salivary gland fragments persist in these animals 24 hours after puparium (no residual salivary gland material is present in wild-type animals at this stage).

      (Nelson et al., 2014)

      Expression of Mmus\Itpr1IP3-sponge.m49.Scer\UAS under the control of Scer\GAL4Gr5a.853 changes the sensitivity of flies to sugars, with expression resulting in less of a preference towards trehalose and an increase in consumption of sucrose.

      Flies expressing Mmus\Itpr1IP3-sponge.m49.Scer\UAS under the control of Scer\GAL4Gr5a.853 exhibit a decrease in trehalose consumption at a variety of trehalose concentration.

      (Usui-Aoki et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The inhibition of autophagy which is seen in the salivary glands 14 hours after pupariation in animals expressing Mmus\Itpr1IP3-sponge.m49.Scer\UAS under the control of Scer\GAL4fkh.PU is still seen if the animals are also mutant for IP3K2Δ1.

      (Nelson et al., 2014)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Mmus\Itpr1IP3-sponge.m49.Scer\UAS
      Mmus\Itpr1IP3-sponge.m49.UAS
      Mmus\Itpr1R441Q.Scer\UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)