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Usui-Aoki, K., Matsumoto, K., Koganezawa, M., Kohatsu, S., Isono, K., Matsubayashi, H., Yamamoto, M.T., Ueda, R., Takahashi, K., Saigo, K., Mikoshiba, K., Yamamoto, D. (2005). Targeted expression of Ip3 sponge and Ip3 dsRNA impaires sugar taste sensation in Drosophila.  J. Neurogenet. 19(3--4): 123--141.
FlyBase ID
FBrf0191035
Publication Type
Research paper
Abstract

We evaluated the role of IP(3) in sugar taste reception in Drosophila melanogaster by inactivating the IP(3) signaling using genetic tools. We used the "IP(3) sponge," composed of the modified ligand-binding domain from the mouse IP(3) receptor, which was designed to absorb IP(3) in competition with native IP(3) receptors. Another tool was a transgene that generates double-stranded RNA against IP(3) receptor mRNA. Both inhibitors diminished the sensitivity of flies to trehalose and sucrose, as estimated by behavioral assays and electrophysiological recordings from the sugar receptor cells. The result indicates that IP(3) signaling is indispensable for sugar reception in Drosophila.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Neurogenet.
    Title
    Journal of Neurogenetics
    Publication Year
    1983-
    ISBN/ISSN
    0167-7063
    Data From Reference
    Aberrations (1)
    Alleles (10)
    Genes (8)
    Natural transposons (1)
    Experimental Tools (2)
    Transgenic Constructs (7)