FB2026_02 , released June 18, 2026
Allele: Dmel\Pak376A
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General Information
Symbol
Dmel\Pak376A
Species
D. melanogaster
Name
FlyBase ID
FBal0263630
Feature type
allele
Associated gene
Dmel\Pak3
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Bahri et al., 2010)
Progenitor genotype
Pak3EP1191
(Bahri et al., 2010)
Cytology
Description

P-element excision results in a small deletion leading to removal of the first 148 codons of the Pak3 A isoform.

(Bahri et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:16,452,467..16,453,762 [-]
Comment:

1296 bp deletion resulting from the imprecise excision of P{EP}Pak3EP1191, extends from the insertion site to 443 bases beyond the ATG.

(Bahri et al., 2010, Bahri et al., 2010)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Pak376A homozygous mutant embryos exhibit various cuticle phenotypes including head holes and defects in the dorsal surface that range from germband retraction failure to dorsal holes and puckers.

      (Bahri et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Pak376A has embryonic/larval cuticle phenotype, enhanceable by Pak14/Pak14

      (Bahri et al., 2010)

      Pak376A has embryonic/larval cuticle phenotype, enhanceable by Pak[+]/Pak14

      (Bahri et al., 2010)
      Suppressed by
      Statement
      Reference

      Pak376A, Pak14 has embryonic/larval cuticle phenotype, suppressible | partially by Pak3UAS.GFP/Scer\GAL4hs.PU

      (Bahri et al., 2010)
      Enhancer of
      Statement
      Reference

      Pak3[+]/Pak376A is an enhancer of embryonic/larval cuticle phenotype of Pak14

      (Bahri et al., 2010)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Pak14 enhances the cuticle phenotypes seen in Pak376A homozygous embryos, with nearly all Pak14 Pak376A double mutants exhibiting head holes. A similar but less severe phenotype is seen in Pak376A homozygous mutants with one copy of Pak14 and Pak14 homozygous mutants with one copy of Pak376A.

      Expression of PakScer\UAS.T:Hsap\MYC under the control of Scer\GAL4hs.PU at 29[o]C rescues the defects in the dorsal surface of the embryonic cuticle seen in Pak14 Pak376A double mutants, but does not effectively suppress the head hole phenotype.

      The dorsal vessel in Pak376A Pak14 double mutant embryos develops normally.

      In Pak376A Pak14 double mutants the opposing leading edge membranes come together in a zig zag pattern following dorsal closure, giving the dorsal midline a cleft-like appearance. In a small number of embryos the epidermal flanks appear to come apart following dorsal closure.

      Unlike in wild type, neither septate or adherens junctions are restored at the leading edge following dorsal closure in Pak376A Pak14 double mutants.

      (Bahri et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Pak376A
      (Bahri et al., 2010)
      pak376A
      (Bahri et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)