W111term | Pak-PA; W111term | Pak-PB; W111term | Pak-PC; W111term | Pak-PE; W111term | Pak-PF; W111term | Pak-PG; W111term | Pak-PH; W111term | Pak-PI; W111term | Pak-PJ
G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.
Pak14 enhances the cuticle phenotypes seen in Pak376A homozygous embryos, with nearly all Pak14 Pak376A double mutants exhibiting head holes. A similar but less severe phenotype is seen in Pak376A homozygous mutants with one copy of Pak14 and Pak14 homozygous mutants with one copy of Pak376A.
Pak14 enhances the cuticle phenotypes seen in Pak327A homozygous embryos. A similar but less severe phenotype is seen in Pak327A homozygous mutants with one copy of Pak14 and Pak14 homozygous mutants with one copy of Pak327A.
Expression of PakScer\UAS.T:Hsap\MYC under the control of Scer\GAL4hs.PU at 29[o]C rescues the defects in the dorsal surface of the embryonic cuticle seen in Pak14 Pak376A double mutants, but does not effectively suppress the head hole phenotype.
In Pak376A Pak14 double mutants the opposing leading edge membranes come together in a zig zag pattern following dorsal closure, giving the dorsal midline a cleft-like appearance. In a small number of embryos the epidermal flanks appear to come apart following dorsal closure.