FlyBase Allele Report: Dmel\Pak[14]

General Information

Symbol

Dmel\Pak¹⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0117681

Feature type

allele

Associated gene

Dmel\Pak

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: W111term.

(Newsome et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:6,356,449..6,356,449 [+]

Nucleotide change:

G6356449A

Amino acid change:

Reported amino acid change:

W111term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Newsome et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Pak¹⁴ follicle cell clones show a penetrant epithelial disorganization phenotype but normal polarity.

(Haack et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Pak[+]/Pak¹⁴ is a suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pak¹⁴ has embryonic/larval cuticle phenotype, enhanceable by Pak3[+]/Pak3^76A

(Bahri et al., 2010)

Suppressed by

Statement

Reference

Pak3^76A, Pak¹⁴ has embryonic/larval cuticle phenotype, suppressible | partially by Pak3^UAS.GFP/Scer\GAL4^hs.PU

(Bahri et al., 2010)

Enhancer of

Statement

Reference

Pak¹⁴/Pak¹⁴ is an enhancer of embryonic/larval cuticle phenotype of Pak3^76A

(Bahri et al., 2010)

Pak[+]/Pak¹⁴ is an enhancer of embryonic/larval cuticle phenotype of Pak3^76A

(Bahri et al., 2010)

Pak¹⁴ is an enhancer of embryonic/larval cuticle phenotype of Pak3^27A

(Bahri et al., 2010)

Suppressor of

Statement

Reference

Pak[+]/Pak¹⁴ is a suppressor of adult mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Other

Statement

Reference

Pak3^76A, Pak¹⁴ has embryonic leading edge cell phenotype

(Bahri et al., 2010)

Pak3^76A, Pak¹⁴ has embryonic dorsal epidermis phenotype

(Bahri et al., 2010)

Pak3^76A, Pak¹⁴ has adherens junction phenotype

(Bahri et al., 2010)

Pak3^76A, Pak¹⁴ has septate junction phenotype

(Bahri et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Pak¹⁴ enhances the cuticle phenotypes seen in Pak3^76A homozygous embryos, with nearly all Pak¹⁴ Pak3^76A double mutants exhibiting head holes. A similar but less severe phenotype is seen in Pak3^76A homozygous mutants with one copy of Pak¹⁴ and Pak¹⁴ homozygous mutants with one copy of Pak3^76A.

Pak¹⁴ enhances the cuticle phenotypes seen in Pak3^27A homozygous embryos. A similar but less severe phenotype is seen in Pak3^27A homozygous mutants with one copy of Pak¹⁴ and Pak¹⁴ homozygous mutants with one copy of Pak3^27A.

Expression of Pak^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.PU at 29[o]C rescues the defects in the dorsal surface of the embryonic cuticle seen in Pak¹⁴ Pak3^76A double mutants, but does not effectively suppress the head hole phenotype.

The dorsal vessel in Pak3^76A Pak¹⁴ double mutant embryos develops normally.

In Pak3^76A Pak¹⁴ double mutants the opposing leading edge membranes come together in a zig zag pattern following dorsal closure, giving the dorsal midline a cleft-like appearance. In a small number of embryos the epidermal flanks appear to come apart following dorsal closure.

Unlike in wild type, neither septate or adherens junctions are restored at the leading edge following dorsal closure in Pak3^76A Pak¹⁴ double mutants.

(Bahri et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9123

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}82B Pak¹⁴/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pak¹⁴

(Baer et al., 2007, Shcherbata et al., 2007)

dpak14

(Bahri et al., 2009)

pak1¹⁴

(Sidor et al., 2020)

pak¹⁴

(Richier et al., 2018, Ihry and Bashirullah, 2014, Haack et al., 2013, Bahri et al., 2010, Ng and Luo, 2004)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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