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General Information
Symbol
Dmel\Pak14
Species
D. melanogaster
Name
FlyBase ID
FBal0117681
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Nucleotide change:

    G6356449A

    Amino acid change:

    W111term | Pak-PA; W111term | Pak-PB; W111term | Pak-PC; W111term | Pak-PE; W111term | Pak-PF; W111term | Pak-PG; W111term | Pak-PH; W111term | Pak-PI; W111term | Pak-PJ

    Reported amino acid change:

    W111term

    Comment:

    G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Amino acid replacement: W111term.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Pak14 follicle cell clones show a penetrant epithelial disorganization phenotype but normal polarity.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressor of
    Statement
    Reference
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference

    Pak14 has embryonic/larval cuticle phenotype, enhanceable by Pak3[+]/Pak376A

    Suppressed by
    Statement
    Reference
    Enhancer of
    Statement
    Reference

    Pak[+]/Pak14 is an enhancer of embryonic/larval cuticle phenotype of Pak376A

    Suppressor of
    Statement
    Reference
    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Pak14 enhances the cuticle phenotypes seen in Pak376A homozygous embryos, with nearly all Pak14 Pak376A double mutants exhibiting head holes. A similar but less severe phenotype is seen in Pak376A homozygous mutants with one copy of Pak14 and Pak14 homozygous mutants with one copy of Pak376A.

    Pak14 enhances the cuticle phenotypes seen in Pak327A homozygous embryos. A similar but less severe phenotype is seen in Pak327A homozygous mutants with one copy of Pak14 and Pak14 homozygous mutants with one copy of Pak327A.

    Expression of PakScer\UAS.T:Hsap\MYC under the control of Scer\GAL4hs.PU at 29[o]C rescues the defects in the dorsal surface of the embryonic cuticle seen in Pak14 Pak376A double mutants, but does not effectively suppress the head hole phenotype.

    The dorsal vessel in Pak376A Pak14 double mutant embryos develops normally.

    In Pak376A Pak14 double mutants the opposing leading edge membranes come together in a zig zag pattern following dorsal closure, giving the dorsal midline a cleft-like appearance. In a small number of embryos the epidermal flanks appear to come apart following dorsal closure.

    Unlike in wild type, neither septate or adherens junctions are restored at the leading edge following dorsal closure in Pak376A Pak14 double mutants.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (4)
    References (11)