FlyBase Allele Report: Crei\ChR2[CHETA(E123T.H134R).UAS.YFP]

General Information

Symbol

Crei\ChR2^{CHETA(E123T.H134R).UAS.YFP}

Species

C. reinhardtii

Name

FlyBase ID

FBal0263912

Feature type

allele

Associated gene

Crei\ChR2

Associated Insertion(s)

Carried in Construct

P{20XUAS-CHETA.YFP}

Also Known As

UAS-ChETA, UAS-ChR2^ChETA, UAS-ChETA::YFP

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Petersen and Stowers, 2011)

Progenitor genotype

Carried in construct

P{20XUAS-CHETA.YFP}

Cytology

Description

20 copies of UAS regulatory sequences drive expression of ChETA(E123T.H134R) tagged with YFP.

(Petersen and Stowers, 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

ChETA(E123T.H134R)

light-regulated neuron activation tool

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | larval stage, with Scer\GAL4^19-12

(Himmel et al., 2023)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing Crei\ChR2^{CHETA.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^Gr5a.8.5 show a complete proboscis extension response when exposed to blue light, including head tilting, extension of the proboscis and opening of the labella.

Flies expressing Crei\ChR2^{CHETA.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^E49 respond to blue light by simply lifting the rostrum out of the head, causing the tip of the proboscis to point upwards.

(Gordon and Scott, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Crei\ChR2^{CHETA(E123T.H134R).UAS.YFP}, Scer\GAL4^ChAT.BAC, VAChT^14Q has abnormal neurophysiology | late embryonic stage | conditional phenotype

(Vernon et al., 2019)

Phenotype Manifest In

Other

Statement

Reference

Crei\ChR2^{CHETA(E123T.H134R).UAS.YFP}, Scer\GAL4^ChAT.BAC, VAChT^14Q has embryonic/larval motor neuron | late embryonic stage | conditional phenotype

(Vernon et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

36354

y¹ w^*; P{20XUAS-CHETA.YFP}attP40/CyO, P{Wee-P.ph0}Bacc^Wee-P20; Dr¹/TM6C, Sb¹ Tb¹

36495

y¹ w^*; wg^Sp-1/CyO, P{Wee-P.ph0}Bacc^Wee-P20; P{20XUAS-CHETA.YFP}attP2/TM6C, Sb¹ Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Crei\ChR2^{CHETA(E123T.H134R).UAS.YFP}

Crei\ChR2^{CHETA.Scer\UAS.T:Avic\GFP-YFP}

Crei\ChR2^{CHETA.UAS.YFP}

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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