Neurons of larval brains expressing Vap-33-1^{T48I.Scer\UAS} under the control of Scer\GAL4^elav.PU are filled with Vap-33-1 inclusions of various sizes, while numerous massive Vap-33-1 aggregates are visible in nerve fibres. Large Vap-33-1 aggregates are also seen in muscle cells when Vap-33-1^{T48I.Scer\UAS} is expressed using Scer\GAL4^C57.

Adult eyes of flies expressing Vap-33-1^{T48I.Scer\UAS} under the control of Scer\GAL4^ey.PU are reduced in size and are rough over the entire surface. Ommatidia are disorganized and fused, with missing, irregular and occasional supernumerary mechano-sensory bristles. Photoreceptor morphology is severely disrupted and several vacuoles are visible. Overall, eye size is less than 50% the size of controls.

Endoplasmic reticulum within larval brain cells expressing Vap-33-1^{T48I.Scer\UAS} under the control of Scer\GAL4^elav.PU appears fragmented and dispersed, rather than the reticular, perinuclear pattern seen in wild type. The same effect is seen in muscle cells when Vap-33-1^{T48I.Scer\UAS} is expressed using Scer\GAL4^C57.