FlyBase Allele Report: Dmel\Sdc[RNAi.UAS.cSa]

General Information

Symbol

Dmel\Sdc^RNAi.UAS.cSa

Species

D. melanogaster

Name

FlyBase ID

FBal0264314

Feature type

allele

Associated gene

Dmel\Sdc

Associated Insertion(s)

Carried in Construct

P{UAS-Sdc.RNAi.S}

Key Links

Transgenic product class

RNAi_reagent

(Schulz et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Schulz et al., 2009)

Mutagen

in vitro construct

(Schulz et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Sdc.RNAi.S}

Cytology

Description

UAS regulatory sequences drive expression of a genomic-cDNA hybrid construct targeting 357bp of Sdc exons 6 and 7.

(Schulz et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Sdc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult dorsal tracheal branch, with Scer\GAL4^btl.PS

(Schulz et al., 2009)

embryonic/larval dorsal tracheal branch | third instar larval stage, with Scer\GAL4^btl.PS

(Schulz et al., 2011)

embryonic/larval dorsal tracheal branch | third instar larval stage, with Scer\GAL4^esg-p127

(Schulz et al., 2011)

Detailed Description

Statement

Reference

In flies expressing Sdc^{dsRNA.Scer\UAS.cSa} under the control of Scer\GAL4^btl.PS the 10 dorsal branches of the tracheal system often fail to establish a dorsal anastomosis at the midline. The frequency of missing anastomoses varies between segments, but overall almost half of all dorsal branches fail to fuse.

Flies expressing Sdc^{dsRNA.Scer\UAS.cSa} in the tracheal fusion cells using Scer\GAL4^esg-p127 have missing dorsal anastomoses in the 10 dorsal branches of the tracheal system.

(Schulz et al., 2011)

Expression of Sdc^{dsRNA.Scer\UAS.cSa} under the control of Scer\GAL4^btl.PS results in a higher rate of unfused tracheal dorsal branch segments per animal compared to wild type.

(Schulz et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^btl.PS, Sdc^RNAi.UAS.cSa has adult dorsal tracheal branch | third instar larval stage phenotype, suppressible by sli²/sli[+]

(Schulz et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^btl.PS, Sdc^RNAi.UAS.cSa has adult dorsal tracheal branch | third instar larval stage phenotype, non-suppressible by lea[+]/robo2⁸

(Schulz et al., 2011)

Enhancer of

Statement

Reference

Sdc^RNAi.UAS.cSa/Scer\GAL4^btl.PS is an enhancer of adult dorsal tracheal branch | third instar larval stage phenotype of Scer\GAL4^btl.PS, robo2^UAS.GFP

(Schulz et al., 2011)

Sdc^RNAi.UAS.cSa/Scer\GAL4^btl.PS is an enhancer of adult dorsal tracheal branch | third instar larval stage phenotype of Scer\GAL4^btl.PS, robo1^UAS.GFP

(Schulz et al., 2011)

Suppressor of

Statement

Reference

Sdc^RNAi.UAS.cSa/Scer\GAL4^btl.PS is a suppressor | partially of adult dorsal tracheal branch | third instar larval stage phenotype of Scer\GAL4^btl.PS, comm^UAS.Tag:MYC

(Schulz et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

sli²/+ suppresses the dorsal branch fusion phenotype seen when Sdc^{dsRNA.Scer\UAS.cSa} is expressed under the control of Scer\GAL4^btl.PS.

lea^robo2-8/+ fails to suppress the dorsal branch fusion phenotype seen when Sdc^{dsRNA.Scer\UAS.cSa} is expressed under the control of Scer\GAL4^btl.PS.

Expression of Sdc^{dsRNA.Scer\UAS.cSa} enhances the dorsal branch fusion phenotype seen when lea^{Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^btl.PS.

Expression of Sdc^{dsRNA.Scer\UAS.cSa} enhances the dorsal branch fusion phenotype seen when robo^{Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^btl.PS.

Expression of Sdc^{dsRNA.Scer\UAS.cSa} partially suppresses the dorsal branch fusion phenotype seen when comm^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^btl.PS.

(Schulz et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Sdc^RNAi.UAS.cSa is rescued by Sdc^RESC.UAS/Scer\GAL4^btl.PS

(Schulz et al., 2011)

Sdc^RNAi.UAS.cSa is rescued by Sdc^RESC.UAS/Scer\GAL4^esg-p127

(Schulz et al., 2011)

Sdc^RNAi.UAS.cSa is rescued by Sdc^RESC.ΔC.UAS/Scer\GAL4^btl.PS

(Schulz et al., 2011)

Sdc^RNAi.UAS.cSa is rescued by Scer\GAL4^btl.PS/Sdc^RESC.B-C.UAS

(Schulz et al., 2011)

Sdc^RNAi.UAS.cSa is rescued by Sdc^RESC.UAS/Scer\GAL4^btl.PS

(Schulz et al., 2009)

Partially rescued by

Sdc^RNAi.UAS.cSa is partially rescued by Scer\GAL4^btl.PS/Sdc^{RESC.Ecto.UAS}

(Schulz et al., 2011)

Not rescued by

Sdc^RNAi.UAS.cSa is not rescued by Sdc^{RESC.ΔGAG.UAS}/Scer\GAL4^btl.PS

(Schulz et al., 2011)

Comments

Expression of Sdc^{RESC.Scer\UAS} rescues the dorsal branch fusion phenotype seen when Sdc^{dsRNA.Scer\UAS.cSa} is expressed under the control of Scer\GAL4^btl.PS.

Expression of Sdc^{RESC.Scer\UAS} rescues the dorsal branch fusion phenotype seen when Sdc^{dsRNA.Scer\UAS.cSa} is expressed under the control of Scer\GAL4^esg-p127.

Expression of Sdc^{RESC.ΔC.Scer\UAS} fully rescues the loss of dorsal branch fusion seen when Sdc^{dsRNA.Scer\UAS.cSa} is expressed under the control of Scer\GAL4^btl.PS.

Expression of Sdc^{RESC.Ecto.Scer\UAS} partially rescues the loss of dorsal branch fusion seen when Sdc^{dsRNA.Scer\UAS.cSa} is expressed under the control of Scer\GAL4^btl.PS.

Expression of Sdc^{RESC.B-C.Scer\UAS} fully rescues the loss of dorsal branch fusion seen when Sdc^{dsRNA.Scer\UAS.cSa} is expressed under the control of Scer\GAL4^btl.PS.

Expression of Sdc^{RESC.ΔGAG.Scer\UAS} fails to rescue the loss of dorsal branch fusion seen when Sdc^{dsRNA.Scer\UAS.cSa} is expressed under the control of Scer\GAL4^btl.PS.

(Schulz et al., 2011)

Expression of Sdc^{RESC.Scer\UAS} rescues the tracheal dorsal branch defects seen when Sdc^{dsRNA.Scer\UAS.cSa} is expressed under the control of Scer\GAL4^btl.PS.

(Schulz et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Sdc^RNAi.UAS.cSa

Sdc^{dsRNA.Scer\UAS.cSa}

Sdc^{dsRNA.UAS.cSa}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close