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General Information
Symbol
Avic\GFPspGFP1-10.UAS.Tag:M(hCD4)
Species
A. victoria
Name
FlyBase ID
FBal0265025
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-CD4::spGFP1-10, UAS-spGFP1-10::CD4, UAS-CD4-spGFP1-10, UAS-spGFP1-10, UAS-splitGFP1-10, UAS-CD4:spGFP1-10
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the spGFP1-10 split fluorescent protein fragment fused to a membrane targeting sequence (Tag:M(hCD4)). This construct is designed for use in a GRASP (GFP Reconstitution Across Synaptic Partners) assay, in which complementary split fluorescent protein fragments expressed on the extracellular membrane of different neurons can reconstitute a functional fluorescent protein at points of contact between the neurons.

Allele components
Product class / Tool use(s)
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (5)
Notes on Origin
Discoverer
Comments
Comments

Avic\GFPspGFP1-10.Scer\UAS.T:Hsap\CD4 forms one half of the split-GFP "GFP reconstitution across synaptic partners" (GRASP) system (see PMID:18255029). In this system two split-GFP halves are expressed on the membrane of distinct neuronal populations. Avic\GFP is reconstituted trans-synaptically and fluorescence is seen when the two neuron populations connect.

Avic\GFPspGFP1-10.Scer\UAS.T:Hsap\CD4 is enclosed within an Scer\FRT cassette in the PBac{FRT(UAS-CD4-spGFP1-10)} construct, so that it can be excised by Scer\FLP1.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Avic\GFPspGFP1-10.Scer\UAS.T:Hsap\CD4
Avic\GFPspGFP1-10.UAS.Tag:M(hCD4)
Name Synonyms
Secondary FlyBase IDs
    References (51)