FlyBase Experimental Tool Report: Tag:M(hCD4)

FB2026_02 , released June 18, 2026

FB2026_02 , released June 18, 2026

Experimental tool: Tag:M(hCD4)

General Information

Symbol

Tag:M(hCD4)

FlyBase ID

FBto0000199

Name

Membrane tethering sequence from CD4

Description

Description

Sequence from the Homo sapiens CD4 gene (HGNC:1678) that results in the tagged protein being tethered to the membrane (PMID:18255029). The tag may comprise the entire CD4 gene or only a part of it.

Uses

membrane protein localization tag

External Crossreferences and Linkouts

Related experimental tools

Transgenic Constructs

Tagged with the tool (86)

Transgenic construct(s)

Component allele

Reg. region

Encoded product / tool

Tagged with

Also carries

Stocks

Avic\GFP^{tdGFP.UAS.Tag:M(hCD4)}

UAS

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag

Disc\RFP^{tdTomato.UAS.Tag:M(hCD4)}

UAS

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{lexAop-CD4-spGFP11}

Avic\GFP^{spGFP11.lexAop.Tag:M(hCD4)}

lexAop

spGFP11: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

Avic\GFP^{spGFP1-10.UAS.Tag:M(hCD4)}

UASt

spGFP1-10: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

Drad\BphP^{IFP2.0.13xlexAop.Tag:M(hCD4)}

lexAop

IFP2.0: infra-red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

Avic\GFP^{tdGFP.13xlexAop.Tag:M(hCD4)}

lexAop

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag

Disc\RFP^{tdTomato.13xlexAop.Tag:M(hCD4)}

lexAop

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

P{UAS-CD4-CLIPf}

Zzzz\CLIP^{f.UAS.Tag:M(hCD4)}

UAS

CLIPf-tag: chemical labeling reporter

Tag:M(hCD4): membrane protein localization tag

P{UAS-CD4-SNAPf}

Zzzz\SNAP^{f.UAS.Tag:M(hCD4)}

UAS

SNAPf-tag: chemical labeling reporter

Tag:M(hCD4): membrane protein localization tag

PBac{10XQUAS-pre-t-GRASP}

cac^{10XQUAS.pre-t-GRASP.spGFP11,Tag:HA}

QUAS

cac

spGFP11: split fluorescent protein
Tag:HA: epitope tag
Tag:M(hCD4): membrane protein localization tag
Tag:TS(rNrxn1-β): protein localization tag

PBac{13XLexAop2-pre-t-GRASP}

cac^{13XLexAop2.pre-t-GRASP.spGFP11,Tag:HA}

lexAop

cac

spGFP11: split fluorescent protein
Tag:HA: epitope tag
Tag:M(hCD4): membrane protein localization tag
Tag:TS(rNrxn1-β): protein localization tag

PBac{20XUAS-pre-t-GRASP}

cac^{20XUAS.pre-t-GRASP.spGFP11,Tag:HA}

UAS

cac

spGFP11: split fluorescent protein
Tag:HA: epitope tag
Tag:M(hCD4): membrane protein localization tag
Tag:TS(rNrxn1-β): protein localization tag

Avic\GFP^{tdGFP.ppk.Tag:M(hCD4)}

ppk

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag

Disc\RFP^{tdTomato.ppk.Tag:M(hCD4)}

ppk

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

Brsp\BphP^{mIFP.UAS.Tag:M(hCD4)}

UAS

mIFP: infra-red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{13xLexAop2(FRT.stop)spGFP11-CD4-HA-T2A-brpD3-mCherry}

Avic\GFP^{spGFP11.13XLexAop2.FRT.Tag:M(hCD4),Tag:HA}

lexAop

spGFP11: split fluorescent protein

Tag:HA: epitope tag
Tag:M(hCD4): membrane protein localization tag

FRT: recombinase target site

P{13xlexAop-HaloTag-CD4}

Rhsp\Halo^{13xlexAop.Tag:SS(pat-3),Tag:M(hCD4)}

lexAop

Halo-tag(Unk): chemical labeling reporter

Tag:M(hCD4): membrane protein localization tag
Tag:SS(pat-3): signal sequence

P{20xUAS-HaloTag-CD4}

Rhsp\Halo^{20xUAS.Tag:M(hCD4)}

UAS

Halo-tag(Unk): chemical labeling reporter

Tag:M(hCD4): membrane protein localization tag
Tag:SS(pat-3): signal sequence

P{GMR-CD4-spGFP11}

Avic\GFP^{spGFP11.GMR.Tag:M(hCD4)}

GMR

spGFP11: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{QUAS-spGFP11}

Avic\GFP^{spGFP11.QUAS.Tag:M(hCD4)}

QUAS

spGFP11: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{R9D03-CD4-tdTom}

Disc\RFP^{tdTomato.R9D03.Tag:M(hCD4)}

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{R9D11-CD4-tdTom}

Disc\RFP^{tdTomato.R9D11.Tag:M(hCD4)}

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{UAS(FRT.rCD2-stop)CD4::tdTomato}

Disc\RFP^{tdTomato.FRT.UAS.rCD2-stop.Tag:M(hCD4)}

UAS

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

FRT: recombinase target site

P{UAS-CD4-spGFP11}

Avic\GFP^{spGFP11.UAS.Tag:M(hCD4)}

UAS

spGFP11: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

M{Dsim\Or67a.3R-CD4-tdGFP}

Avic\GFP^{tdGFP.Dsim\Or67a.3R.Tag:M(hCD4)}

Dsim\Or67a.3R

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

P{10xUAS-CD4-APEX2-V5}

Gmax\APX1^{APEX2.10xUAS.Tag:M(hCD4),Tag:V5}

UAS

APEX2: reporter enzyme
proximity labeling tool (protein)

Tag:V5: epitope tag
Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

Avic\GFP^{GCaMP6f.IVS.10xUAS.Tag:M(hCD4)}

UAS

GCaMP6f: calcium ion sensor
fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{13XLexAop2(FRT.STOP)CD4-spGFP11}

Avic\GFP^{spGFP11.FRT.13xlexAop.Tag:M(hCD4)}

lexAop

spGFP11: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

FRT: recombinase target site

P{13XLexAop2(-FRT)CD4-spGFP11}

Avic\GFP^{spGFP11.-FRT.13xlexAop.Tag:M(hCD4)}

lexAop

spGFP11: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

FRT: recombinase target site

P{13xLexAop2(-FRT)spGFP11-CD4-HA-T2A-brpD3-mCherry}

Avic\GFP^{spGFP11.13XLexAop2.Tag:M(hCD4).Tag:HA}

lexAop

spGFP11: split fluorescent protein

Tag:HA: epitope tag
Tag:M(hCD4): membrane protein localization tag

P{13xlexAop-IVS-pre-mGRASP}

Avic\GFP^{spGFP11.13xlexAop.pre-mGRASP}

lexAop

spGFP11: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:TS(rNrxn1-β): protein localization tag

P{DsecIr75b-CD4::tdGFP}

Avic\GFP^{tdGFP.Dsec\Ir75b.Tag:M(hCD4}

Dsec\Ir75b

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

P{DsimIr75b-CD4::tdGFP}

Avic\GFP^{tdGFP.Dsim\Ir75b.Tag:M(hCD4}

Dsim\Ir75b

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

P{erm-CD4-tdTom.B}

Disc\RFP^{tdTom.erm(B).Tag:M(hCD4)}

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{erm-CD4-tdTom.R1}

Disc\RFP^{tdTom.erm(R1).Tag:M(hCD4)}

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{erm-CD4-tdTom.R2}

Disc\RFP^{tdTom.erm(R2).Tag:M(hCD4)}

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{fog-CD4-tdGFP.int-enh2}

Avic\GFP^{tdGFP.fog.int-enh2.Tag:M(hCD4)}

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{fog-CD4-tdGFP.int-enh3}

Avic\GFP^{tdGFP.fog.int-enh3.Tag:M(hCD4)}

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{fog-CD4-tdTom.int-enh1}

Disc\RFP^{tdTomato.fog.int-enh1.Tag:M(hCD4)}

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{Ir31a-CD4::tdTom}

Disc\RFP^{tdTomato.Ir31a.Tag:M(hCD4)}

Ir31a

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

P{Ir75a-CD4::tdGFP}

Avic\GFP^{tdGFP.Ir75a.Tag:M(hCD4}

Ir75a

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

P{Ir75b-CD4::tdGFP}

Avic\GFP^{tdGFP.Ir75b.Tag:M(hCD4}

Ir75b

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

P{Ir75b-CD4::tdTom}

Disc\RFP^{tdTomato.Ir75b.Tag:M(hCD4)}

Ir75b

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

P{Ir75c-CD4::tdGFP}

Avic\GFP^{tdGFP.Ir75c.Tag:M(hCD4}

Ir75c

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

P{lexAop-CD4-spGFP1-10.K}

Avic\GFP^{spGFP1-10.lexAop.cKa.Tag:M(hCD4)}

lexAop

spGFP1-10: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{lexAop-CD4-spGFP1-10}

Avic\GFP^{spGFP1-10.lexAop.Tag:M(hCD4)}

lexAop

spGFP1-10: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{mb247-CD4-spGFP11}

Avic\GFP^{spGFP11.Mef2.mb247.Tag:M(hCD4)}

Mef2

spGFP11: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{parS(INT2).dpn-1xhbintron-mCD4-GFP}

Avic\GFP^{tdGFP.dpn.1xhbint.Tag:M(hCD4)}

dpn

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{parS(INT2).dpn-3xhbintron-mCD4-GFP}

Avic\GFP^{tdGFP.dpn.3xhbint.Tag:M(hCD4)}

dpn

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{parS(INT2).dpn-IVS-mCD4-GFP}

Avic\GFP^{tdGFP.dpn.Tag:M(hCD4)}

dpn

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{ppk-MApHS}

Hsap\CD4^MApHS.ppk

ppk

pHluorinE: pH sensor
green fluorescent protein
tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{ppk-spGFP11-CD4-tdTom}

Disc\RFP^{tdTomato.ppk.spGFP11,Tag:M(hCD4)}

ppk

tdTomato: red fluorescent protein

spGFP11: split fluorescent protein
Tag:M(hCD4): membrane protein localization tag

P{QUAS-CD4-spGFP11.Z}

Avic\GFP^{spGFP11.QUAS.Tag:M(hCD4).cZa}

QUAS

spGFP11: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{QUAS-CD4-spGFP11}

Avic\GFP^{spGFP11.QUAS.Tag:M(hCD4).cMa}

QUAS

spGFP11: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{R9D11ΔbHLH-O3-CD4-tdTom}

Disc\RFP^{tdTom.R9D11ΔbHLH-O3.Tag:M(hCD4)}

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{R9D11ΔbHLH-O123-CD4-tdTom}

Disc\RFP^{tdTom.R9D11ΔbHLH-O123.Tag:M(hCD4)}

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{R10D05-CD4-tdTom}

Disc\RFP^{tdTomato.R10D05.Tag:M(hCD4)}

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

P{R20C11-CD4-tdGFP}

Avic\GFP^{tdGFP.R20C11.Tag:M(hCD4)}

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

P{UAS(-FRT)CD4::tdTomato}

Disc\RFP^{tdTomato.-FRT.UAS.Tag:M(hCD4)}

UAS

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{UAS-CD4-spCer1-10}

Avic\GFP^{spCer1-10.UAS.Tag:M(hCD4)}

UAS

spCer1-10: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{UAS-CD4-spCFP1-10}

Avic\GFP^{spCFP1-10.UAS.Tag:M(hCD4)}

UASt

spCFP1-10: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{UAS-CD4-spGFP11.K}

Avic\GFP^{spGFP11.UAS.cKa.Tag:M(hCD4)}

UASt

spGFP11: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{UAS-CD4-tdTom.S}

Disc\RFP^{tdTomato.UAS.cSa.Tag:M(hCD4)}

UAS

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{UAS-DIP-α-CD4}

DIP-α^{N.UAS.Tag:M(hCD4)}

UASt

DIP-α

Tag:M(hCD4): membrane protein localization tag

P{UAS-MApHS}

Hsap\CD4^MApHS.UAS

UAS

pHluorinE: pH sensor
green fluorescent protein
tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

P{UAS-pHluorinSE-CD4-tdTomato}

Avic\GFP^{pHluorinSE.UAS.tdTomato,Tag:M(hCD4)}

UAS

pHluorinSE: pH sensor
fluorescent protein
tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

PBac{10XQUAS-pre-GRASP-brp}

brp^{10XQUAS.pre-GRASP.spGFP11,Tag:HA}

QUAS

brp

spGFP11: split fluorescent protein
Tag:HA: epitope tag
Tag:M(hCD4): membrane protein localization tag
Tag:TS(rNrxn1-β): protein localization tag

PBac{10XQUAS-pre-GRASP-cacWT}

cac^{WT.10XQUAS.pre-GRASP.spGFP11,Tag:HA}

QUAS

cac

spGFP11: split fluorescent protein
Tag:HA: epitope tag
Tag:M(hCD4): membrane protein localization tag
Tag:TS(rNrxn1-β): protein localization tag

PBac{10XQUAS-pre-GRASP-Fife}

Fife^{10XQUAS.pre-GRASP.spGFP11,Tag:HA}

QUAS

Fife

spGFP11: split fluorescent protein
Tag:HA: epitope tag
Tag:M(hCD4): membrane protein localization tag
Tag:TS(rNrxn1-β): protein localization tag

PBac{10XQUAS-pre-GRASP-Lar}

Lar^{10XQUAS.pre-GRASP.spGFP11,Tag:HA}

QUAS

Lar

spGFP11: split fluorescent protein
Tag:HA: epitope tag
Tag:M(hCD4): membrane protein localization tag
Tag:TS(rNrxn1-β): protein localization tag

PBac{10XQUAS-pre-GRASP-Rab3}

Rab3^{10XQUAS.pre-GRASP.spGFP11,Tag:HA}

QUAS

Rab3

spGFP11: split fluorescent protein
Tag:HA: epitope tag
Tag:M(hCD4): membrane protein localization tag
Tag:TS(rNrxn1-β): protein localization tag

PBac{10XQUAS-pre-GRASP-Rbp}

Rbp^{10XQUAS.pre-GRASP.spGFP11,Tag:HA}

QUAS

Rbp

spGFP11: split fluorescent protein
Tag:HA: epitope tag
Tag:M(hCD4): membrane protein localization tag
Tag:TS(rNrxn1-β): protein localization tag

PBac{10XQUAS-pre-mGRASP-V5}

Avic\GFP^{spGFP11.10XQUAS.pre-mGRASP.Tag:V5}

QUAS

spGFP11: split fluorescent protein

Tag:V5: epitope tag
Tag:M(hCD4): membrane protein localization tag
Tag:TS(rNrxn1-β): protein localization tag

PBac{10xUAS-CD4-spGFP1-10}

Avic\GFP^{spGFP1-10.10xUAS.Tag:M(hCD4)}

UAS

spGFP1-10: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

PBac{10xUAS-IVS-pre-mGRASP}

Avic\GFP^{spGFP11.10xUAS.pre-mGRASP}

UAS

spGFP11: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:TS(rNrxn1-β): protein localization tag

PBac{13xlexAop-CD4-spGFP11}

Avic\GFP^{spGFP11.13xlexAop.Tag:M(hCD4)}

lexAop

spGFP11: split fluorescent protein

Tag:M(hCD4): membrane protein localization tag

Disc\RFP^{Dsim\Or67a.D.PA}

Dsim\Or67a.D

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

Avic\GFP^{tdGFP.Dsim\Or67a.P.Tag:M(hCD4)}

Dsim\Or67a.P

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

PBac{Ir84a-CD4::tdGFP}

Avic\GFP^{tdGFP.Ir84a.Tag:M(hCD4}

Ir84a

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

PBac{ppk-CD4-GFP.PHzICH}

Avic\GFP^{ppk.PHzICH.Tag:M(hCD4)}

ppk

GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag

PBac{tup-CD4-tdGFP.AME-R}

Avic\GFP^{tdGFP.tup.AME-R.Tag:M(hCD4)}

EGFP: green fluorescent protein
GFP: green fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

Disc\RFP^{tdTomato.tup.AME-R.Tag:M(hCD4)}

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag
Tag:SS(Akh): signal sequence

Drad\BphP^{IFP2.0.UAS.Tag:M(hCD4)}

UAS

IFP2.0: infra-red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

PBac{UAS-CD4-iRFP713}

Rpal\BphP2^{iRFP713.UAS.Tag:M(hCD4)}

UAS

Rpal\BphP2

Tag:M(hCD4): membrane protein localization tag

PBac{UAS-CD4-tdTom.K}

Disc\RFP^{tdTomato.UAS.cKa.Tag:M(hCD4)}

UAS

tdTomato: red fluorescent protein

Tag:M(hCD4): membrane protein localization tag

PBac{UAS-dpr10-CD4.V5}

dpr10^{N.UAS.Tag:M(hCD4).Tag:V5}

UASt

dpr10

Tag:V5: epitope tag
Tag:M(hCD4): membrane protein localization tag
Tag:SS(BiP): signal sequence

Insertions ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tag:M(hCD4)

Name Synonyms

Membrane tethering sequence from CD4

Secondary FlyBase IDs

References (40)

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