FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{ppk-CD4-tdTom}
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General Information
Symbol
P{ppk-CD4-tdTom}
FlyBase ID
FBtp0068014
Feature type
transgenic_transposable_element
Component Allele(s)
Disc\RFPtdTomato.ppk.Tag:M(hCD4)
Also Known As
ppk-CD4-tdTomato, ppk-CD4-tdTom
Size
Expression Data
Associated insertion(s)
3 available
Molecular map
Description and Uses
Construct components
Component allele Disc\RFPtdTomato.ppk.Tag:M(hCD4)
Transgenic product class
Component
Use(s)
Regulatory region(s)
ppk
Encoded product / tool
tdTomato
Tagged with
Tag:M(hCD4)
Description

A ppk enhancer followed by the core promoter and 5' UTR of Hsp70 is fused upstream of the z second intron followed by sequences encoding the transmembrane domain of Hsap\CD4 fused to tdTomato. An endoplasmic reticulum export signal from Hsap\KCNJ2 is fused to the C-terminus of the tdTomato sequences. 3'UTR and polyA sequences from His2Av are present downstream of the coding region.

(Han et al., 2011)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
larval multidendritic class IV neuron
(Han et al., 2012)
abdominal dorsal multidendritic neuron ddaC
(Han et al., 2012)
abdominal anterior ventral multidendritic neuron vdaa
(Han et al., 2012)
abdominal ventral multidendritic neuron vdaB
(Han et al., 2012)
Additional Information
Statement
Reference

DiscRFPtdTomato.ppk.Tag:M(hCD4) is expressed in all larval class IV dendritic arborizing neurons.

(Han et al., 2012)
 
Marker for
larval multidendritic class IV neuron
(Han et al., 2012)
Reflects expression of
ppk
(Han et al., 2012)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
Bloomington
35844
w1118; P{ppk-CD4-tdTom}4a
35845
w1118; P{ppk-CD4-tdTom}10a/TM6B, Tb1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Symbol Synonym
CD4::tdTom
(Riebli et al., 2013)
P{ppk-CD4-tdTom}
(FlyBase, 1996-)
ppk-CD4-tdTOM
(Sung et al., 2024)
ppk-CD4-tdTom
(Han et al., 2011)
ppk-CD4-tdTomato
(Luedke et al., 2024, Del Castillo et al., 2022, Li et al., 2022, Jiang et al., 2019)
ppk-CD4::Tomato
(Kelliher et al., 2018)
ppk-CD4::tdTomato
(Mitchell et al., 2023)
ppk-cd4-tdtom
(Liu et al., 2022)
Secondary FlyBase IDs
    References (13)