FlyBase Recombinant Construct Report: PBac{FRT(UAS-CD4-spGFP1-10)}

UASt regulatory sequences drive expression of the spGFP1-10 split fluorescent protein fragment fused to a membrane targeting sequence (Tag:M(hCD4)). This construct is designed for use in a GRASP (GFP Reconstitution Across Synaptic Partners) assay, in which complementary split fluorescent protein fragments expressed on the extracellular membrane of different neurons can reconstitute a functional fluorescent protein at points of contact between the neurons.

(Gordon and Scott, 2009)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Avic\GFP^{spGFP1-10.UAS.Tag:M(hCD4)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Avic\GFP^{spGFP1-10.Scer\UAS.T:Hsap\CD4} is enclosed within an Scer\FRT cassette in the PBac{FRT(UAS-CD4-spGFP1-10)} construct, so that it can be excised by Scer\FLP1.

(Cavanaugh et al., 2014)

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

80706

w¹¹¹⁸; PBac{FRT(UAS-CD4-spGFP1-10)}VK00033/TM6C, Sb¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

FRT-UAS-GFP1-10-FRT

(Cavanaugh et al., 2014)

PBac{FRT(UAS-CD4-spGFP1-10)}

(FlyBase, 1996-)

UAS-FRT-GFP1-10-FRT

(Cavanaugh et al., 2014)

Secondary FlyBase IDs

References (4)

Report Sections

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