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General Information
Symbol
pHluorinE
FlyBase ID
FBto0000112
Name
ecliptic pHluorin
Description
Description

pHluorinE is a pH-sensitive green fluorescent protein. It gradually loses fluorescence as pH is lowered, until at pH values of less than 6.0, the excitation peak at 475nm vanishes. In an environment of pH less than 6.0, the protein is therefore invisible (eclipsed) under 475nm excitation, but can still be weakly seen at 395nm. These changes are reversible within less than 20ms after returning to neutral pH. It is an artificial derivative of the naturally occurring fluorescent protein encoded by the Aequorea victoria GFP gene (UniProtKB:P42212), containing the mutations S147D, N149Q, T161I, V163A, S175G, S202F, Q204T and A206T (PMID:9671304).

External Crossreferences and Linkouts
FPbase - A database for users of fluorescent proteins.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Related experimental tools
Common gene of origin (64)
Tool
Uses
Transgenic Constructs
Insertions ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
T:Avic\GFPpHluorinE
pHluorinE
Name Synonyms
ecliptic pHluorin
Secondary FlyBase IDs
  • FBal0189780
References (7)