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General Information
Symbol
PA-GFP
FlyBase ID
FBto0000057
Name
Photoactivatable green fluorescent protein
Description
Description

The PA-GFP entry in FlyBase represents a photoactivatable green fluorescent protein which is derived from the naturally occurring fluorescent protein encoded by the Aequorea victoria GFP gene (UniProtKB:P42212) and which is photoactivatable due to the presence of a T203H mutation in the chromophore (PMID:12228718). Upon activation with UV light (390-415 nm), PA-GFP is converted from a weakly fluorescent protein to a bright green fluorescent state with an excitation peak of 504nm and an emission peak of 517nm in vitro, resulting in a 100-fold increase in green fluorescence (PMID:12228718, PMID:20664080, PMID:18057027). The A206K mutation that disrupts dimerization (PMID:11988576) may also be present, resulting in a monomeric form if this is case.

External Crossreferences and Linkouts
FPbase - A database for users of fluorescent proteins.
Related experimental tools
Common gene of origin (64)
Tool
Uses
Transgenic Constructs
Encodes tool (5)
Tagged with the tool (9)
Insertions ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
PA-GFP
T:Avic\GFPPA.cMa
T:Avic\GFPPA
T:Avic\GFPpa
Name Synonyms
Photoactivatable GFP
Photoactivatable green fluorescent protein
Secondary FlyBase IDs
  • FBal0247467
  • FBal0219754
  • FBal0181328
References (14)