FlyBase Allele Report: Ecol\lexA[VP16.Mef2.247]

General Information

Symbol

Ecol\lexA^{VP16.Mef2.247}

Species

E. coli

Name

FlyBase ID

FBal0265180

Feature type

allele

Associated gene

Ecol\lexA

Associated Insertion(s)

Carried in Construct

P{Mef2-LexA-VP16.247}

Also Known As

MB247-lexA, 247-LexA, 247-LexA::VP16, mb247-LexA::VP16

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Pitman et al., 2011)

in vitro construct - coding region fusion

(Pitman et al., 2011)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{Mef2-LexA-VP16.247}

Cytology

Nature of the lesion

Statement

Reference

The 247bp Mef2 mushroom body enhancer regulates expression of a lexA::VP16 driver.

(Pitman et al., 2011)

Allele components

Product class / Tool use(s)

Regulatory region(s)

Mef2

Encoded product / tool

lexA::VP16

binary expression system - driver

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

Kenyon cell

(Perisse et al., 2013)

Additional Information

Statement

Reference

Marker for

Kenyon cell

(Perisse et al., 2013)

Reflects expression of

Mef2

(Perisse et al., 2013)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | adult stage | conditional, with Hsod\aop3^{eArch3.0.lexAop.EYFP}

(Liu et al., 2016)

abnormal neurophysiology | adult stage | conditional, with Npha\HR^{eNpHR3.0.lexAop.EYFP}

(Liu et al., 2016)

Phenotype Manifest In

mushroom body output neuron 3 | conditional, with Hsod\aop3^{eArch3.0.lexAop.EYFP}

(Liu et al., 2016)

mushroom body output neuron 3 | conditional, with Npha\HR^{eNpHR3.0.lexAop.EYFP}

(Liu et al., 2016)

Detailed Description

Statement

Reference

Optogenetic stimulation (using Hsod\aop3^{e.lexAop.EYFP} or Npha\HR^{e.lexAop.EYFP} with 593 nm (yellow) light activation) of Ecol\lexA^{VP16.Mef2.247} neurons results in hyperpolarisation of beta'2mp (medial lobe arborizing neuron 4) cells.

(Liu et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Gthe\ACR1^{lexAop.d.EYFP}/Ecol\lexA^{VP16.Mef2.247} is a non-enhancer of abnormal locomotor behavior | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4

(Stern et al., 2019)

Suppressor of

Statement

Reference

Gthe\ACR1^{lexAop.d.EYFP}/Ecol\lexA^{VP16.Mef2.247} is a suppressor | conditional | partially of abnormal locomotor behavior | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4, norpA³⁶

(Stern et al., 2019)

NOT Suppressor of

Statement

Reference

Gthe\ACR1^{lexAop.d.EYFP}/Ecol\lexA^{VP16.Mef2.247} is a non-suppressor of abnormal locomotor behavior | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4

(Stern et al., 2019)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ecol\lexA^{Mef2.247.T:Hsim\VP16}

Ecol\lexA^{VP16.Mef2.247}

Name Synonyms

Secondary FlyBase IDs

References (22)

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