FlyBase Allele Report: Ecol\lexA[VP16.Mef2.247]

General Information

Symbol

Ecol\lexA^{VP16.Mef2.247}

Species

E. coli

Name

FlyBase ID

FBal0265180

Feature type

allele

Associated gene

Ecol\lexA

Associated Insertion(s)

Carried in Construct

P{Mef2-LexA-VP16.247}

Also Known As

MB247-lexA, 247-LexA, mb247-LexA::VP16, 247-LexA::VP16, MB-LexA

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pitman et al., 2011)

Progenitor genotype

Carried in construct

P{Mef2-LexA-VP16.247}

Cytology

Description

The 247bp Mef2 mushroom body enhancer regulates expression of a lexA::VP16 driver.

(Pitman et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

Mef2

Encoded product / tool

lexA::VP16

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

larval Kenyon cell

(Awasaki et al., 2011)

third instar larval stage

larval Kenyon cell

(Mohamed et al., 2023)

P-stage

Kenyon cell

(Awasaki et al., 2011)

adult stage

Kenyon cell

(Perisse et al., 2013)

Additional Information

Statement

Reference

Drives expression in Kenyon cells.

(FlyBase Curators, 2017)

Marker for

Kenyon cell

(Perisse et al., 2013)

Reflects expression of

Mef2

(Perisse et al., 2013, Awasaki et al., 2011)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | adult stage | conditional, with Hsod\aop3^{eArch3.0.lexAop.EYFP}

(Liu et al., 2016)

abnormal neurophysiology | adult stage | conditional, with Npha\HR^{eNpHR3.0.lexAop.EYFP}

(Liu et al., 2016)

Phenotype Manifest In

mushroom body output neuron 3 | conditional, with Hsod\aop3^{eArch3.0.lexAop.EYFP}

(Liu et al., 2016)

mushroom body output neuron 3 | conditional, with Npha\HR^{eNpHR3.0.lexAop.EYFP}

(Liu et al., 2016)

Detailed Description

Statement

Reference

Optogenetic stimulation (using Hsod\aop3^{e.lexAop.EYFP} or Npha\HR^{e.lexAop.EYFP} with 593 nm (yellow) light activation) of Ecol\lexA^{VP16.Mef2.247} neurons results in hyperpolarisation of beta'2mp (medial lobe arborizing neuron 4) cells.

(Liu et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Gthe\ACR1^{lexAop.d.EYFP}/Ecol\lexA^{VP16.Mef2.247} is a non-enhancer of abnormal locomotor behavior | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4

(Stern et al., 2019)

Suppressor of

Statement

Reference

Gthe\ACR1^{lexAop.d.EYFP}/Ecol\lexA^{VP16.Mef2.247} is a suppressor | conditional | partially of abnormal locomotor behavior | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4, norpA^P24

(Stern et al., 2019)

NOT Suppressor of

Statement

Reference

Gthe\ACR1^{lexAop.d.EYFP}/Ecol\lexA^{VP16.Mef2.247} is a non-suppressor of abnormal locomotor behavior | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4

(Stern et al., 2019)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

606105

w^*; l(2)*^*/CyO; P{Mef2-LexA-VP16.247}3/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ecol\lexA^{Mef2.247.T:Hsim\VP16}

Ecol\lexA^{VP16.Mef2.247}

Mef2^LexA

(Raouf Issa et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (36)

Report Sections

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