FlyBase Allele Report: Dmel\stg[hs.PE3]

General Information

Symbol

Dmel\stg^hs.PE3

Species

D. melanogaster

Name

heat shock construct of Edgar

FlyBase ID

FBal0265405

Feature type

allele

Associated gene

Dmel\stg

Associated Insertion(s)

Carried in Construct

P{hs-stg.WH1}

Also Known As

hs-stg

Key Links

Transgenic product class

wild_type

(Edgar and O'Farrell, 1990)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Edgar and O'Farrell, 1990)

Mutagen

in vitro construct

(Schittenhelm et al., 2010, Milan et al., 1996, Knoblich and Lehner, 1993, Edgar and O'Farrell, 1990)

Progenitor genotype

Carried in construct

P{hs-stg.WH1}

Cytology

Description

stg gene expression is under the control of Hsp70 promoter.

(Edgar and O'Farrell, 1990)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

stg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased occurrence of cell division | embryonic stage

(Edgar and O'Farrell, 1990)

increased occurrence of cell division | pupal stage

(Milan et al., 1996)

lethal | recessive

(Edgar and O'Farrell, 1990)

Phenotype Manifest In

eye disc | larval stage

(Baker and Yu, 2001)

wing blade

(Milan et al., 1996)

wing cell

(Milan et al., 1996)

wing margin

(Milan et al., 1996)

Detailed Description

Statement

Reference

Ectopic expression of stg^hs.PE3 using heat shock causes cells in the eye disc to enter mitosis.

(Baker and Yu, 2001)

Expression of stg^hs.PE3 in stg² embryos which are arrested in G2 of cycle 14 (after a 3 hour arrest in G2) induces synchronous mitoses. The spindles in these induced mitoses show no significant abnormalities. Preanaphase figures have one centrosome at each spindle pole.

(Vidwans et al., 1999)

Expression by heat shock of stg^hs.PE3 in pupae 4-8 hours after puparium formation causes almost all wing cells to enter into mitosis, with the exception of the wing margin cells. Two hours after the heat shock and extra mitosis, only a small fraction of the cells enter into S phase. These cells appear in clusters distributed along the longitudinal veins. 4 to 16 hours after the heat shock, the pattern of cells in S phase is similar to wild-type, although the fraction of cells in S phase is reduced compared to wild-type. Adult wings derived from pupae heat shocked 4-8 hours after puparium formation show no increase in size compared to wild-type. Expression by heat shock of stg^hs.PE3 in pupae 20-24 hours after puparium formation induces extra mitoses in the wing which are not followed by an extra S phase.

(Milan et al., 1996)

Heat shock during larval stages has no effect on the phenotype of the eyes of resulting adults.

(Richardson et al., 1995)

Heat induced expression restores spindle formation in twe¹ mutant testis.

(Sigrist et al., 1995)

Heat induced expression of stg resulted in premature and almost perfectly synchronous mitosis 16 in CycB-deficient embryos, however subsequent progression through mitosis is slower than for wild type.

(Knoblich and Lehner, 1993)

Heat shock 160-185 minutes after egg deposition causes the mitotic times of all embryonic cells, except those in the amnioserosa and mitotic domain B that are normally nondividing, to advance. The length of the S phase is unaltered. Heat shocked stg proteins can only induce mitotic divisions in G2 cells. Heat shock of G2 cells of cycle 14 causes all cells, including those from the amnioserosa, to enter S phase. By varying the time of heat pulses cells can only be induced to divide very early in G2 of interphase 16.

(Edgar and O'Farrell, 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

Egfr^E3, stg^hs.PE3 has eye-antennal disc phenotype

(Kylsten and Saint, 1997)

Egfr^E3, stg^hs.PE3 has wing disc phenotype

(Kylsten and Saint, 1997)

Additional Comments

Genetic Interactions

Statement

Reference

25 minutes of heat induced stg^hs.PE3 expression in Egfr^E3 larvae causes mitotic cell appearance in the eye and wing discs. Induction of mitosis in the eye discs leads to a marked reduction of pyknotic bodies that are normally present on the basal side of the discs. Cellular progression into and through apoptosis is disrupted by ectopic stg expression. Heat induced stg expression does not affect the mutant eye phenotype: adults exhibit small elliptical and rough eyes as seen in Egfr^E3 homozygotes.

(Kylsten and Saint, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

91703

w^*; P{hs-stg.WH1}3.2

Notes on Origin

Discoverer

B. Edgar.

(Knoblich and Lehner, 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

stg^hs.PE3

stg^hs.PH

Name Synonyms

heat shock construct of Edgar

(Edgar and O'Farrell, 1990)

heat shock construct of Herzig

Secondary FlyBase IDs

FBal0032817
FBal0247235

References (20)

Report Sections

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