FlyBase Allele Report: Dmel\gce[2.5k]

General Information

Symbol

Dmel\gce^2.5k

Species

D. melanogaster

Name

FlyBase ID

FBal0266011

Feature type

allele

Associated gene

Dmel\gce

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

transposable element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Abdou et al., 2011)

Mutagen

transposable element activity

(Abdou et al., 2011)

Progenitor genotype

Mi{ET1}gce^MB07696

(Abdou et al., 2011)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 2.5kb deletion that removes exons 5-8 and part of exon 9 of gce.

(Abdou et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant | larval stage

(Abdou et al., 2011)

decreased fecundity | female

(Abdou et al., 2011)

female semi-sterile

(Abdou et al., 2011)

viable

(Abdou et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant larvae are resistant to the juvenile hormone agonist pyriproxyfen compared to wild type.

Mutant females show reduced fecundity: they begin ovipositing at 4.6 days after eclosion (in contrast to 3.0 days for wild-type females) and after 30 days, the total number of eggs laid by a single mutant female is only 65% that of wild type. The hatching rate of the mutant eggs is only 88%.

(Abdou et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Met²⁷, gce^2.5k has some die during P-stage phenotype, suppressible | partially by Met^{+tCH321-28O05}

(Baumann et al., 2017)

Met²⁷, gce^2.5k has some die during P-stage phenotype, suppressible by gce^{+tCH321-09E09}

(Baumann et al., 2017)

Met²⁷, gce^2.5k has lethal phenotype, suppressible by Scer\GAL4^arm.PU/Met^UAS.cJa

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, suppressible by Scer\GAL4^Tub.PU/Met^UAS.cJa

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, suppressible by gce^UAS.cJa/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, suppressible by gce^UAS.cJa/Scer\GAL4^Tub.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, suppressible by gce^UAS.Tag:FLAG/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, suppressible by gce^UAS.Tag:FLAG/Scer\GAL4^Tub.PU

(Jindra et al., 2015)

NOT suppressed by

Statement

Reference

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^T272Y.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^T272Y.UAS/Scer\GAL4^Tub.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^{T272Y.UAS.Tag:FLAG}/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^{T272Y.UAS.Tag:FLAG}/Scer\GAL4^Tub.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^{V315F.UAS.Tag:FLAG}/Scer\GAL4^Tub.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^V315F.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by Scer\GAL4^Tub.PU/gce^V315F.UAS

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^C366M.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^C366M.UAS/Scer\GAL4^Tub.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by Met^V449F.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by Scer\GAL4^Tub.PU/Met^V449F.UAS

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by Met^C500M.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by Scer\GAL4^Tub.PU/Met^C500M.UAS

(Jindra et al., 2015)

Suppressor of

Statement

Reference

Met¹, gce^2.5k, Met²⁷, gce^MI02742 is a suppressor | partially of abnormal cell migration | parental effect | embryonic stage phenotype of Hmgcr^UAS.cvDa, Scer\GAL4^48Y

(Barton et al., 2024)

Other

Statement

Reference

Met¹/Met²⁷, gce^2.5k/gce^MI02742 has partially lethal - majority die phenotype

(Barton et al., 2024)

Met¹/Met²⁷, gce^2.5k/gce^MI02742 has decreased fecundity phenotype

(Barton et al., 2024)

Met¹/Met²⁷, gce^2.5k/gce^MI02742 has abnormal cell migration | maternal effect | embryonic stage phenotype

(Barton et al., 2024)

Met²⁷, gce^2.5k has some die during P-stage phenotype

(Baumann et al., 2017)

Met^GD4384, Scer\GAL4^Act5C.PI, gce^2.5k has majority die during P-stage phenotype

(Baumann et al., 2017)

Scer\GAL4^phtm.PO, gce^2.5k has abnormal developmental rate phenotype

(Baumann et al., 2017)

Met²⁷, gce^2.5k has lethal phenotype

(Jindra et al., 2015)

Met²⁷, gce^2.5k has increased cell death phenotype

(Abdou et al., 2011)

Met²⁷, gce^2.5k has decreased body size | larval stage phenotype

(Abdou et al., 2011)

Met²⁷, gce^2.5k has abnormal developmental rate | larval stage phenotype

(Abdou et al., 2011)

Met²⁷, gce^2.5k has lethal | P-stage phenotype

(Abdou et al., 2011)

Met²⁷, gce^2.5k has decreased body size | P-stage phenotype

(Abdou et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

Met¹, gce^2.5k, Met²⁷, gce^MI02742 is a suppressor | partially of primordial germ cell | parental effect | embryonic stage phenotype of Hmgcr^UAS.cvDa, Scer\GAL4^48Y

(Barton et al., 2024)

Other

Statement

Reference

Met¹/Met²⁷, gce^2.5k/gce^MI02742 has primordial germ cell | maternal effect | embryonic stage phenotype

(Barton et al., 2024)

Met²⁷, gce^2.5k has fat body phenotype

(Abdou et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

gce^2.5k mutants expressing Met^GD4384 under the control of Scer\GAL4^Act5C.PI exhibit nearly 100% pupal lethality.

gce^2.5k mutants expressing Scer\GAL4^phm.PO exhibit a developmental delay as compared to controls.

Met²⁷, gce^2.5k double mutant females (crossed to control males) show 25.9% pupal lethality; this lethality is fully rescued upon expression of gce^{+tCH321-09E09}, but only partially rescued upon expression of Met^{+tCH321-28O05}.

(Baumann et al., 2017)

Onset of metamorphosis (larval wandering stage) is delayed approximately 12 hours compared to wild type in Met²⁷ gce^2.5k double mutant larvae. Double mutant larvae and early pupae have a reduced body size compared to controls. The double mutants fail to undergo head eversion and die approximately 24 hours after pupariation. Fat body cells in the double mutants undergo precocious and enhanced programmed cell death compared to wild-type fat body cells.

(Abdou et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

The lethality of Met²⁷ gce^2.5k double mutants is fully rescued by a single copy of Met^+t5.7 or by expression of gce^Scer\UAS.cBa under the control of Scer\GAL4^arm.PS.

(Abdou et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

90981

w^* v^* Met^W3 gce^2.5k/FM7c, P{2xTb¹-RFP}FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Gce^2.5k

(Wu et al., 2021)

gce^2.5K

(Liu et al., 2018)

gce^2.5k

(Barton et al., 2024, Baumann et al., 2017, Jia et al., 2017, Jindra et al., 2015, Wen et al., 2015, He et al., 2014, Abdou et al., 2011)

gce^25k

(Arquier et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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