FlyBase Allele Report: Dmel\stwl[UY823]

General Information

Symbol

Dmel\stwl^UY823

Species

D. melanogaster

Name

FlyBase ID

FBal0266036

Feature type

allele

Associated gene

Dmel\stwl

Associated Insertion(s)

P{Mae-UAS.6.11}stwl^UY823

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{Mae-UAS.6.11}stwl^UY823

Cytology

Description

Insertion at position +111 in the first exon of stwl. The insertion is oriented such that the ATG codon at position -3 in the inserted P{Mae-UAS.6.11} element is in frame with the coding sequence of stwl, allowing overexpression of a putative protein with the first 37 stwl amino acids deleted in the presence of a Scer\GAL4 driver.

(Brun et al., 2006)

Allele components

Component

Use(s)

Inserted element

P{Mae-UAS.6.11}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | larval stage

(Brun et al., 2006)

female sterile (with stwl^j6C3)

(Brun et al., 2006)

female sterile (with stwl^Δ95)

(Brun et al., 2006)

female sterile | recessive

(Brun et al., 2006)

hyperplasia, with Scer\GAL4^en.PU

(Brun et al., 2006)

hyperplasia, with Scer\GAL4^vg.PM

(Brun et al., 2006)

increased cell death, with Scer\GAL4^en.PU

(Brun et al., 2006)

lethal | heat sensitive, with Scer\GAL4^da.G32

(Brun et al., 2006)

lethal | heat sensitive, with Scer\GAL4^en.PU

(Brun et al., 2006)

lethal | heat sensitive, with Scer\GAL4^GMR.PF

(Brun et al., 2006)

lethal - all die before end of pupal stage | heat sensitive, with Scer\GAL4^vg.PM

(Brun et al., 2006)

some die during pupal stage | heat sensitive, with Scer\GAL4^vg.PM

(Brun et al., 2006)

Phenotype Manifest In

egg chamber

(Brun et al., 2006)

embryonic/larval salivary gland | larval stage, with Scer\GAL4^fkh.PZ

(Brun et al., 2006)

eye, with Scer\GAL4^GMR.PF

(Brun et al., 2006)

ovary

(Brun et al., 2006)

wing disc, with Scer\GAL4^en.PU

(Brun et al., 2006)

wing disc, with Scer\GAL4^vg.PM

(Brun et al., 2006)

wing hinge, with Scer\GAL4^vg.PM

(Brun et al., 2006)

wing hinge primordium, with Scer\GAL4^vg.PM

(Brun et al., 2006)

wing margin, with Scer\GAL4^en.PU

(Brun et al., 2006)

wing margin, with Scer\GAL4^vg.PM

(Brun et al., 2006)

wing pouch, with Scer\GAL4^en.PU

(Brun et al., 2006)

wing pouch, with Scer\GAL4^vg.PM

(Brun et al., 2006)

Detailed Description

Statement

Reference

Homozygous females are sterile and do not lay eggs. The females have rudimentary ovaries depleted of post-vitellogenic eggs, but the germaria are hypertrophied, containing abnormal egg chambers.

Expression of stwl^UY823 under the control of Scer\GAL4^en.PU at 21[o]C or 25[o]C results in lethality. At 18[o]C, 25.6% of animals survive to adulthood. These escapers have notches in the wing margin.

Expression of stwl^UY823 under the control of Scer\GAL4^vg.PM results in lethality at 25[o]C. At lower temperatures, some animals survive to adulthood. These escapers have notches in the wing margin and prominent hyperplastic buds on the wing hinge.

Expression of stwl^UY823 under the control of Scer\GAL4^GMR.PF results in lethality at 25[o]C. At low 21[o]C, some escapers are seen, which have glassy eyes. At 18[o]C, viability is 81.4%, and the adults have rough eyes.

The hinge and wing pouch regions of wing discs expressing stwl^UY823 under the control of Scer\GAL4^vg.PM have multiple, prominent folds resulting from an excess of cells.

Wing discs expressing stwl^UY823 under the control of Scer\GAL4^en.PU show massive hyperplasia, with the posterior part of the disc being much larger than the posterior part of control discs. Cells in the posterior part of the disc show increased BrdU labelling compared to anterior cells, indicating an increase in the number of cells in S phase. Cells in the "zone of nonproliferating cells" (ZNC) in the posterior part of the wing pouch are labelled with BrdU, in contrast to the ZNC cells in the anterior part of the wing pouch. Apoptosis is seen in the posterior part of the wing disc.

Larvae expressing stwl^UY823 under the control of Scer\GAL4^fkh.PZ have smaller salivary glands than normal. The salivary gland cells and their nuclei are also much smaller than normal.

(Brun et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^vg.PM, stwl^UY823 has lethal | pupal stage phenotype, suppressible by Mmus\Bax^UAS.cGa, Scer\GAL4^vg.PM

(Brun et al., 2006)

Scer\GAL4^vg.PM, stwl^UY823 has lethal | pupal stage phenotype, suppressible | partially by hid^UAS.cZa/Scer\GAL4^vg.PM

(Brun et al., 2006)

Scer\GAL4^vg.PM, stwl^UY823 has lethal | pupal stage phenotype, suppressible | partially by Scer\GAL4^vg.PM/rpr^UAS.cZa

(Brun et al., 2006)

Scer\GAL4^vg.PM, stwl^UY823 has lethal | pupal stage phenotype, suppressible | partially by p53^GUS.PB/Scer\GAL4^vg.PM

(Brun et al., 2006)

Scer\GAL4^vg.PM, stwl^UY823 has lethal | pupal stage phenotype, suppressible | partially by grim^UAS.cNa/Scer\GAL4^vg.PM

(Brun et al., 2006)

Scer\GAL4^vg.PM, stwl^UY823 has lethal | pupal stage phenotype, suppressible | partially by skl^UAS.cSa/Scer\GAL4^vg.PM

(Brun et al., 2006)

NOT suppressed by

Statement

Reference

Scer\GAL4^vg.PM, stwl^UY823 has lethal | pupal stage phenotype, non-suppressible by Diap1^UAS.cHa, Scer\GAL4^vg.PM

(Brun et al., 2006)

Scer\GAL4^vg.PM, stwl^UY823 has lethal | pupal stage phenotype, non-suppressible by dap^UAS.cLa/Scer\GAL4^vg.PM

(Brun et al., 2006)

Scer\GAL4^vg.PM, stwl^UY823 has lethal | pupal stage phenotype, non-suppressible by Hsap\BCL2^UAS.cGa, Scer\GAL4^vg.PM

(Brun et al., 2006)

Scer\GAL4^vg.PM, stwl^UY823 has lethal | pupal stage phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^vg.PM

(Brun et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^vg.PM, stwl^UY823 has wing margin phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^vg.PM

(Brun et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa suppresses wing notching phenotype which is seen in flies expressing stwl^UY823 under the control of Scer\GAL4^vg.PM at 21[o]C.

(Brun et al., 2006)

Complementation and Rescue Data

Fails to complement

stwl^j6C3

(Brun et al., 2006)

stwl^Δ95

(Brun et al., 2006)

Rescued by

stwl^UY823 is rescued by stwl^+mCa

(Brun et al., 2006)

Comments

stwl^+mCa rescues the sterility of homozygous stwl^UY823 females.

(Brun et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

stwl^UY823

(Brun et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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