FlyBase Allele Report: Dmel\p53[GUS.PB]

General Information

Symbol

Dmel\p53^GUS.PB

Species

D. melanogaster

Name

FlyBase ID

FBal0135653

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

P{GUS-p53}

Also Known As

GUS-p53, GUS-dp53, p53^WT, UAS-p53, UAS-dp53

Key Links

Transgenic product class

wild_type

(Brodsky, 2002.5.14)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Brodsky, 2002.5.14)

Mutagen

in vitro construct

(Brodsky, 2002.5.14)

Progenitor genotype

Carried in construct

P{GUS-p53}

Cytology

Description

Full length, wild type ORF of p53 is expressed from both GMR and UAS regulatory sequences.

(Brodsky, 2002.5.14)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

UAS

binary expression system - regulatory region

Encoded product / tool

p53

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^GMR.PU

(Li et al., 2017, Fan et al., 2010)

abnormal starvation stress response, with Scer\GAL4^yolk

(Barrio et al., 2014)

decreased cell number | pupal stage | somatic clone, with Scer\GAL4^GMR.PU

(Fan et al., 2010)

decreased cell number | third instar larval stage, with Scer\GAL4^GMR.PU

(Fan et al., 2010)

increased cell death, with Scer\GAL4^Bx-MS1096

(Merino et al., 2002)

increased cell death, with Scer\GAL4^en-e16E

(McEwen and Peifer, 2005)

increased cell death | third instar larval stage, with Scer\GAL4^GMR.PU

(Fan et al., 2010)

lethal, with Scer\GAL4^elav-C155

(Bauer et al., 2005)

visible, with Scer\GAL4^Bx-MS1096

(Merino et al., 2002)

visible, with Scer\GAL4^GMR.PF

(Kang et al., 2012, Moon et al., 2008)

visible, with Scer\GAL4^GMR.PU

(Griswold et al., 2008, Brodsky et al., 2004)

visible | adult stage, with Scer\GAL4^GMR.PU

(Li et al., 2017, Fan et al., 2010)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Kang et al., 2012, Moon et al., 2008)

eye, with Scer\GAL4^GMR.PU

(Li et al., 2017, Fan et al., 2010, Griswold et al., 2008, Brodsky et al., 2004)

eye disc, with Scer\GAL4^GMR.PF

(Moon et al., 2008)

eye disc | posterior | third instar larval stage, with Scer\GAL4^GMR.PU

(Fan et al., 2010)

ommatidium | pupal stage | somatic clone, with Scer\GAL4^GMR.PU

(Fan et al., 2010)

photoreceptor cell R7 | pupal stage | somatic clone, with Scer\GAL4^GMR.PU

(Fan et al., 2010)

photoreceptor cell R7 | third instar larval stage, with Scer\GAL4^GMR.PU

(Fan et al., 2010)

wing, with Scer\GAL4^Bx-MS1096

(Merino et al., 2002)

Detailed Description

Statement

Reference

Expressing p53^GUS.PB under the control of Scer\GAL4^GMR.PU induces a small, rough eye phenotype.

(Li et al., 2017)

Expression of p53^GUS.PB in the fat body under the control of Scer\GAL4^yolk increase the starvation resistance of adult flies.

(Barrio et al., 2014)

Expression of p53^GUS.PB under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype.

(Kang et al., 2012)

Expression of p53^GUS.PB under the control of Scer\GAL4^GMR.PU produces flies with small and rough eyes with a glossy appearance. Massive cell death is seen in the posterior half of the third instar larval eye disc and the number of R7 photoreceptors is reduced compared to wild type. Pupal eye discs also show disrupted ommatidial organization and a reduction in the number of R7 photoreceptors.

(Fan et al., 2010)

Scer\GAL4^GMR.PF-driven overexpression of p53^GUS.PB induces apoptosis in the developing eye disc, giving a rough eye phenotype that can be suppressed by co-expression of p53^259H.GUS.

(Moon et al., 2008)

Expression of p53^GUS.PB in adult neurons, driven by Scer\GAL4^{elav.Switch.PO} following induction with RU486, does not affect lifespan.

(Bauer et al., 2005)

Expression of p53^GUS.PB in the posterior compartment of the wing disc, driven by Scer\GAL4^en-e16E, induces apoptosis.

(McEwen and Peifer, 2005)

Flies expressing p53^GUS.PB under the control of Scer\GAL4^GMR.PU exhibit a rough eye phenotype.

(Brodsky et al., 2004)

Hemocytes in @p53^GUS.PB

; Scer\GAL4^srp.Hemo@ late embryos do not exhibit abnormal aggregation.

(Bruckner et al., 2004)

Expression of p53^GUS.PB under the control of Scer\GAL4^Bx-MS1096 results in abnormally shaped wings with a dramatic reduction of the wing blade, due to massive cell death.

(Merino et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, p53^GUS.PB has abnormal size | adult stage phenotype, enhanceable by mir-285^{UAS.DsRed(Unk)}/Scer\GAL4^GMR.PU

(Li et al., 2017)

Scer\GAL4^GMR.PU, p53^GUS.PB has visible | adult stage phenotype, enhanceable by mir-285^{UAS.DsRed(Unk)}/Scer\GAL4^GMR.PU

(Li et al., 2017)

Scer\GAL4^GMR.PU, p53^GUS.PB has visible phenotype, enhanceable by lok^GUS.cBa/Scer\GAL4^GMR.PU

(Brodsky et al., 2004)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, p53^GUS.PB has visible phenotype, non-enhanceable by Scer\GAL4^GMR.PF, p53^GUS.PB

(Kang et al., 2012)

Scer\GAL4^GMR.PF, p53^GUS.PB has visible phenotype, non-enhanceable by Rbf^UAS.cDa, Scer\GAL4^GMR.PF

(Moon et al., 2008)

Scer\GAL4^GMR.PU, p53^GUS.PB has visible phenotype, non-enhanceable by grp^GUS.cBa/Scer\GAL4^GMR.PU

(Brodsky et al., 2004)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, p53^GUS.PB has increased cell death | third instar larval stage phenotype, suppressible | somatic clone by Df(3L)H99

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has increased cell death | third instar larval stage phenotype, suppressible | somatic clone by Dronc^I29

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has increased cell death | third instar larval stage phenotype, suppressible | partially by hid^rvX1/Df(3L)H99

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has increased cell death | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PU

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has abnormal size phenotype, suppressible | partially by dap^UAS.cdNa/Scer\GAL4^GMR.PU

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has visible phenotype, suppressible | partially by dap^UAS.cdNa/Scer\GAL4^GMR.PU

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has increased cell death | third instar larval stage phenotype, suppressible | partially by dap^UAS.cdNa/Scer\GAL4^GMR.PU

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has visible phenotype, suppressible by lok^kd.GUS/Scer\GAL4^GMR.PU

(Brodsky et al., 2004)

Scer\GAL4^Bx-MS1096, p53^GUS.PB has visible phenotype, suppressible by hay[+]/hay^nc2rv1

(Merino et al., 2002)

Scer\GAL4^Bx-MS1096, p53^GUS.PB has visible phenotype, suppressible by hay[+]/hay^nc2

(Merino et al., 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, p53^GUS.PB has visible phenotype, non-suppressible by Scer\GAL4^GMR.PF, p53^GUS.PB

(Kang et al., 2012)

Scer\GAL4^GMR.PU, p53^GUS.PB has decreased cell number | third instar larval stage phenotype, non-suppressible | somatic clone by Df(3L)H99

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has decreased cell number | pupal stage phenotype, non-suppressible | somatic clone by Df(3L)H99

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has decreased cell number | third instar larval stage phenotype, non-suppressible | somatic clone by Dronc^I29

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has increased cell death | third instar larval stage phenotype, non-suppressible by Df(3L)H99/Df(3L)XR38

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has increased cell death | third instar larval stage phenotype, non-suppressible by Df(3L)H99/rpr⁸⁷

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has abnormal size phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PU

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has visible phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PU

(Fan et al., 2010)

Scer\GAL4^GMR.PF, p53^GUS.PB has visible phenotype, non-suppressible by Rbf^UAS.cDa, Scer\GAL4^GMR.PF

(Moon et al., 2008)

Scer\GAL4^en-e16E, p53^GUS.PB has increased cell death phenotype, non-suppressible by Scer\GAL4^en-e16E/puc^UASp.Tag:MYC

(McEwen and Peifer, 2005)

Scer\GAL4^GMR.PU, p53^GUS.PB has visible phenotype, non-suppressible by grp^GUS.cBa/Scer\GAL4^GMR.PU

(Brodsky et al., 2004)

Enhancer of

Statement

Reference

p53^GUS.PB/Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Scer\GAL4^GMR.PU, mir-285^{UAS.DsRed(Unk)}

(Li et al., 2017)

NOT Enhancer of

Statement

Reference

Scer\GAL4^GMR.PF, p53^GUS.PB is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, p53^GUS.PB

(Kang et al., 2012)

Suppressor of

Statement

Reference

p53^GUS.PB/Scer\GAL4^vg.PM is a suppressor | partially of lethal | pupal stage phenotype of Scer\GAL4^vg.PM, stwl^UY823

(Brun et al., 2006)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF, p53^GUS.PB is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, p53^GUS.PB

(Kang et al., 2012)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^GMR.PU, p53^GUS.PB has decreased cell number | somatic clone | third instar larval stage phenotype

(Fan et al., 2010)

Scer\GAL4^GMR.PU, lok^GUS.cBa, p53^GUS.PB has some die during pupal stage | heat sensitive phenotype

(Brodsky et al., 2004)

Scer\GAL4^GMR.PU, lok^GUS.cBa, p53^GUS.PB has lethal - all die before end of pupal stage | heat sensitive phenotype

(Brodsky et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, p53^GUS.PB has eye phenotype, enhanceable by mir-285^{UAS.DsRed(Unk)}/Scer\GAL4^GMR.PU

(Li et al., 2017)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, p53^GUS.PB has eye phenotype, non-enhanceable by Scer\GAL4^GMR.PF, p53^GUS.PB

(Kang et al., 2012)

Scer\GAL4^GMR.PF, p53^GUS.PB has eye phenotype, non-enhanceable by Rbf^UAS.cDa, Scer\GAL4^GMR.PF

(Moon et al., 2008)

Scer\GAL4^GMR.PF, p53^GUS.PB has eye disc phenotype, non-enhanceable by Dp^unspecified, Scer\GAL4^GMR.PF

(Moon et al., 2008)

Scer\GAL4^GMR.PU, p53^GUS.PB has eye phenotype, non-enhanceable by grp^GUS.cBa/Scer\GAL4^GMR.PU

(Brodsky et al., 2004)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, p53^GUS.PB has eye disc | posterior | third instar larval stage phenotype, suppressible | somatic clone by Df(3L)H99

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has eye disc | posterior | third instar larval stage phenotype, suppressible | somatic clone by Dronc^I29

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has eye disc | posterior | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PU

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has eye disc | posterior | third instar larval stage phenotype, suppressible | partially by dap^UAS.cdNa/Scer\GAL4^GMR.PU

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has photoreceptor cell R7 | third instar larval stage phenotype, suppressible | partially by dap^UAS.cdNa/Scer\GAL4^GMR.PU

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has eye phenotype, suppressible | partially by dap^UAS.cdNa/Scer\GAL4^GMR.PU

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has eye phenotype, suppressible by lok^kd.GUS/Scer\GAL4^GMR.PU

(Brodsky et al., 2004)

Scer\GAL4^GMR.PU, p53^GUS.PB has eye phenotype, suppressible by lok^GUS.cBa/Scer\GAL4^GMR.PU

(Brodsky et al., 2004)

Scer\GAL4^Bx-MS1096, p53^GUS.PB has wing phenotype, suppressible by hay[+]/hay^nc2

(Merino et al., 2002)

Scer\GAL4^Bx-MS1096, p53^GUS.PB has wing phenotype, suppressible by hay[+]/hay^nc2rv1

(Merino et al., 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, p53^GUS.PB has eye phenotype, non-suppressible by Scer\GAL4^GMR.PF, p53^GUS.PB

(Kang et al., 2012)

Scer\GAL4^GMR.PU, p53^GUS.PB has eye disc | posterior | third instar larval stage phenotype, non-suppressible by hid^rvX1/Df(3L)H99

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has eye phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PU

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has photoreceptor cell R7 | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Df(3L)H99

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has ommatidium | somatic clone | pupal stage phenotype, non-suppressible | somatic clone by Df(3L)H99

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has photoreceptor cell R7 | somatic clone | pupal stage phenotype, non-suppressible | somatic clone by Df(3L)H99

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has photoreceptor cell R7 | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Dronc^I29

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has eye disc | posterior | third instar larval stage phenotype, non-suppressible by Df(3L)H99/Df(3L)XR38

(Fan et al., 2010)

Scer\GAL4^GMR.PU, p53^GUS.PB has eye disc | posterior | third instar larval stage phenotype, non-suppressible by Df(3L)H99/rpr⁸⁷

(Fan et al., 2010)

Scer\GAL4^GMR.PF, p53^GUS.PB has eye phenotype, non-suppressible by Rbf^UAS.cDa, Scer\GAL4^GMR.PF

(Moon et al., 2008)

Scer\GAL4^GMR.PF, p53^GUS.PB has eye disc phenotype, non-suppressible by Dp^unspecified, Scer\GAL4^GMR.PF

(Moon et al., 2008)

Scer\GAL4^GMR.PU, p53^GUS.PB has eye phenotype, non-suppressible by grp^GUS.cBa/Scer\GAL4^GMR.PU

(Brodsky et al., 2004)

Enhancer of

Statement

Reference

p53^GUS.PB/Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, mir-285^{UAS.DsRed(Unk)}

(Li et al., 2017)

NOT Enhancer of

Statement

Reference

Scer\GAL4^GMR.PF, p53^GUS.PB is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, p53^GUS.PB

(Kang et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF, p53^GUS.PB is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, p53^GUS.PB

(Kang et al., 2012)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^GMR.PU, p53^GUS.PB has photoreceptor | somatic clone | third instar larval stage phenotype

(Fan et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Cdk5^GD13840 does not affect the rough eye phenotype caused by expression of p53^GUS.PB under the control of Scer\GAL4^GMR.PF.

(Kang et al., 2012)

Induction of Df(3L)H99 third instar larval eyes disc clones blocks the cell death seen when p53^GUS.PB is expressed under the control of Scer\GAL4^GMR.PU. The reduction in R7 photoreceptors is not rescued. Df(3L)H99 is also unable to rescue the ommatidial organisation and reduction in R7 cell number seen in pupal eye discs.

Induction of Nc^I29 third instar larval eyes disc clones blocks the cell death seen when p53^GUS.PB is expressed under the control of Scer\GAL4^GMR.PU. However the reduction in R7 photoreceptors is not rescued.

A Df(3L)XR38/Df(3L)H99 background does not suppress the cell death seen when p53^GUS.PB is expressed in third instar eye discs under the control of Scer\GAL4^GMR.PU.

A rpr⁸⁷/Df(3L)H99 background does not suppress the cell death seen when p53^GUS.PB is expressed in third instar eye discs under the control of Scer\GAL4^GMR.PU.

A W^rvX1/Df(3L)H99 background significantly suppresses the cell death seen when p53^GUS.PB is expressed in third instar eye discs under the control of Scer\GAL4^GMR.PU.

(Fan et al., 2010)

The eye phenotype resulting from the overexpression of p53^GUS.PB via Scer\GAL4^GMR.PF is unaffected by the co-expression of Rbf^Scer\UAS.cDa.

The apoptosis induced by the overexpression of p53^GUS.PB via Scer\GAL4^GMR.PFin the eye disc is unaffected by homozygous Dp^unspecified.

(Moon et al., 2008)

Coexpression of puc^{Scer\UAS.P\T.T:Hsap\MYC} does not block the apoptosis induced in the wing disc by expression of p53^GUS.PB under the control of Scer\GAL4^en-e16E.

(McEwen and Peifer, 2005)

Expression of grp^GUS.cBa has no effect on the rough eye phenotype seen when p53^GUS.PB is expressed under the control of Scer\GAL4^GMR.PU.

Expression of lok^kd.GUS suppresses the rough eye phenotype seen when p53^GUS.PB is expressed under the control of Scer\GAL4^GMR.PU.

When raised at 25[o]C, animals co-expressing p53^GUS.PB and lok^GUS.cBa under the control of Scer\GAL4^GMR.PU die during pupation. When raised at 18[o]C, expression of lok^GUS.cBa enhances the rough eye phenotype seen in flies expressing p53^GUS.PB alone.

(Brodsky et al., 2004)

The wing defects caused by expression of p53^GUS.PB under the control of Scer\GAL4^Bx-MS1096 are completely suppressed by hay^nc2/+ or hay^nc2rv1/+.

(Merino et al., 2002)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa is unable to suppress the small, rough eye phenotype seen when p53^GUS.PB is expressed under the control of Scer\GAL4^GMR.PU. However the cell death seen in the posterior half of the p53^GUS.PB third instar larval eye disc is strongly inhibited.

Co-expression of p53^GUS.PB and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PU results in loss of all types of photoreceptor neurons.

Expression of dap^{Scer\UAS.cdNa} partially rescues the cell death and R7 photoreceptor loss seen when p53^GUS.PB is expressed in third instar larval eye discs under the control of Scer\GAL4^GMR.PU. The adult eye ablation phenotype is also partially rescued.

(Fan et al., 2010)

Complementation and Rescue Data

Rescues

p53^GUS.PB rescues p53^-ns

(Barrio et al., 2014)

p53^GUS.PB rescues p53^11-1B-1

(Brodsky et al., 2004)

Comments

Expression of p53^GUS.PB under the control of Scer\GAL4^yolk rescues the reduced survival seen in homozygous p53^-ns flies in response to starvation.

(Barrio et al., 2014)

Expression of p53^GUS.PB rescues the reduction in X-ray induced radiation seen in p53^11-1B-1 mutant third instar larvae. This rescue is accomplished in the absence of a Scer\GAL4 driver.

(Brodsky et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6584

w¹¹¹⁸; P{GUS-p53}2.1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

p53^GUS.PB

p53^WT

(Nandakumar et al., 2020, Flegel et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (23)

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