Expression of Ten-mScer\UAS.cZa under the control of one copy of Scer\GAL469B does not cause defects in routing of the intersegmental nerve (ISN) axon. However, expression driven by two copies of Scer\GAL469B results in ISN migration defects in 55% of embryos, including abnormal defasciculation, segmental boundary crossovers and bifurcations at the termini.
Expression of Ten-mScer\UAS.cZa under the control of two copies of Scer\GAL4prd.RG1 results in ISN migration defects in 50% of embryos in the abdominal segments in which Ten-mScer\UAS.cZa is expressed.