FB2026_02 , released June 18, 2026
Allele: Dmel\nst16923
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General Information
Symbol
Dmel\nst16923
Species
D. melanogaster
Name
FlyBase ID
FBal0267101
Feature type
allele
Associated gene
Dmel\nst
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Mariappa et al., 2011)
Progenitor genotype
Cytology
Description

An 8 base pair duplication, leading to a frameshift producing a premature STOP codon after Asn218.

(Mariappa et al., 2011)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos maternally and zygotically mutant for nst16923 exhibit defects in mesoderm layer formation during gastrulation, and generation of the dorsal tracheal trunk is completely abolished. Differentiation of eve-positive dorsal mesoderm precursors is also affected in embryos maternally and zygotically mutant for nst16923, but is unaffected in zygotic mutants.

      The amount of UDP-HexNAc in embryos derived from nst16923 germline clones is only 17% of that in wild type embryos. Global O-GlcNAcylation is also reduced.

      Injection of GlcNAcstatin C (a small molecular inhibitor of Oga that promotes O-GlcNAcylation) into embryos derived from nst16923 germline clones suppresses the mesoderm defect and significantly increases the number of eve-positive dorsal mesoderm precursors compared to controls.

      (Mariappa et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      nst16923 has mesoderm | germline clone phenotype, suppressible | partially by sxcUAS.Tag:HA/Scer\GAL4twi.PU

      (Mariappa et al., 2011)
      NOT suppressed by
      Statement
      Reference

      nst16923 has mesoderm | germline clone phenotype, non-suppressible by Scer\GAL4twi.PU/htlλ.UAS

      (Mariappa et al., 2011)
      Enhancer of
      Statement
      Reference

      nst16923 is an enhancer of mesoderm phenotype of htlYY262

      (Mariappa et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      nst16923 zygotically enhances the reduced eve-positive dorsal mesoderm precursor phenotype of htlYY262 embryos.

      Expression of htlScer\UAS.T:λ\cI-DD using Scer\GAL4twi.PU fails to rescue mesoderm differentiation in embryos derived from nst16923 germline clones.

      Expression of sxcScer\UAS.T:Ivir\HA1 using Scer\GAL4twi.PU partially suppresses the mesoderm phenotype of embryos derived from nst16923 germline clones.

      (Mariappa et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      nst16923 is rescued by nsthsp.PM

      (Mariappa et al., 2011)

      nst16923 is rescued by nstUAS.Tag:HA/Scer\GAL4twi.PU

      (Mariappa et al., 2011)

      nst16923 is rescued by nstUAS.Tag:HA/Scer\GAL4btl.PS

      (Mariappa et al., 2011)
      Not rescued by

      nst16923 is not rescued by nstS86A.UAS.Tag:HA/Scer\GAL4twi.PU

      (Mariappa et al., 2011)

      nst16923 is not rescued by nstS86A.UAS.Tag:HA/Scer\GAL4btl.PS

      (Mariappa et al., 2011)
      Comments

      Expression of nsthsp.PM rescues the mesoderm and tracheal phenotypes, as well as the lethality, of nst16923 germline clone-derived embryos.

      nsthsp.PM restores UDP-HexNAc levels in nst16923 embryos to 80% that of wild type.

      Expression of nstScer\UAS.T:Ivir\HA1 using Scer\GAL4twi.PU or Scer\GAL4btl.PS suppresses the mesoderm and tracheal defects, respectively, of embryos derived from nst16923 germline clones.

      Expression of nstS86A.Scer\UAS.T:Ivir\HA1 using Scer\GAL4twi.PU or Scer\GAL4btl.PS does not suppress the mesoderm or tracheal defects, respectively, of embryos derived from nst16923 germline clones.

      (Mariappa et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      nst16923
      (Mariappa et al., 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)