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General Information
Symbol
Dmel\htlλ.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0095354
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-λhtl, UAS-HtlAct, λ-Htl, UAS::λhtl, UAS-λ-Htl, UAS-htlCA
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an activated form of htl in which the extracellular domain of htl has been replaced with the dimerization domain of the bacteriophage λ cI repressor (to induce constitutive dimerization of the encoded protein).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Third instar larvae expressing htlλ.UAS under the control of Scer\GAL4repo.PU, Scer\GAL446F or Scer\GAL4Gli.PU exhibit bulges along peripheral nerves (particularly A3 and A4 nerves), which contain increased nuclei numbers respectively from glial, perineurial glial and subperineurial glial cells, as compared to controls.

Clonal expression of htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4-FRT.alrm in astrocytes results in an increase in the volume of the astrocytic domain compared to controls.

Expression of htlScer\UAS.T:λ\cI-DD in retinal basal glial cell (RBGs) clones under the control of Scer\GAL4repo results in extensive proliferation of the RBG cells, without affecting their migration.

Expression of htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4Mef2.PR results in a strongly increased number of progenitors/nephrocytes and cardioblasts in the embryo.

Expression of htlScer\UAS.T:λ\cI-DD using Scer\GAL4twi.PU leads to an expansion of dorsal cell fates in the mesoderm.

Expression of htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4C855a does not cause any mutant phenotype in the larval optic lobe.

Expression of htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4repo results in an increase in glial cell number and impaired glial migration in the eye disc.

Expression of htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4Mz97 increases wrapping of the photoreceptor axons and of the axons of Bolwig's nerve by wrapping glia.

Expression of htlλ.UAS in larvae under the control of Scer\GAL4repo results in a thickened optic stalk due to increased numbers of glial cells; there is little or no overmigration of glial cells along the Bolwig nerve, compared to controls.

Expression of htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL41151 leads to an increase in the number of founder cells and, consequentially, an increase in the number of muscle fibers in the abdomen. The excess founder cells produced are longer and more elongated than wild-type founder cells. In contrast, there is only a moderate increase in the number of myoblasts.

Expression of htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4GMR.PF has no effect on adult eye morphology.

Clones of btlScer\UAS.T:λ\cI-DD (driven by Scer\GAL4αTub84B.PC) in the adult abdomen do not have any effect on polarity.

Expression of htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4slbo.2.6 has no effect on border cell migration.

Expression of htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4dpp.blk1 has no effect on furrow formation in the eye disc.

htlScer\UAS.T:λ\cI-DD expressed under the control of Scer\GAL4twi.PG in a wild-type background has no effect on mesoderm migration.

When expression is driven by Scer\GAL4Bx-MS1096, L5 is broadened and an extra vein appears, crossing between L1 and L2.

Embryos expressing htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4twi.PG develop an increased number of eve-expressing muscle and cardiac progenitors compared to wild-type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
Statement
Reference

Scer\GAL4MS1075/htlλ.UAS is an enhancer of axon & mechanosensory neuron & adult head | conditional ts phenotype of Nrgl10

Suppressor of
Statement
Reference

Scer\GAL4MS1075/htlλ.UAS is a suppressor | partially of axon & adult head | conditional ts phenotype of Nrgl10

NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Expression of htlScer\UAS.T:λ\cI-DD using Scer\GAL4twi.PU fails to rescue mesoderm differentiation in embryos derived from nst16923 germline clones.

Both mesoderm spreading and differentiation in pbl3 homozygous embryos are partially rescued by htlScer\UAS.T:λ\cI-DD; Scer\GAL4twi.PB.

Partially rescues the mesoderm migration defects of sfl03844 embryos derived from sfl03844 female germline clones (lacking both maternal and zygotic sfl function), when expressed under the control of Scer\GAL4twi.PG. Partially rescues the mesoderm migration defects of sgl08310 embryos derived from sgl08310 female germline clones (lacking both maternal and zygotic sgl function), when expressed under the control of Scer\GAL4twi.PG.

The strength of the wing vein phenotype caused by expression of htlScer\UAS.T:λ\cI-DD driven by Scer\GAL4Bx-MS1096 is reduced by co-expression of styScer\UAS.cHa.

The increased number of eve-expressing muscle and cardiac progenitors seen in embryos expressing htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4twi.PG is dominantly suppressed by stumpsYY202. htlScer\UAS.T:λ\cI-DD does not rescue the mesodermal phenotype of stumpsYY202 embryos when expressed under the control of Scer\GAL4twi.PG.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4alrm.PD partially restores infiltration of astrocyte processes into the neuropil in htlAB42 embryos, however, migration of the ventral-most astrocyte is not rescued.

Expression of htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4tey-5053A rescues the longitudinal visceral muscle (LVM) founder cell death seen in htlAB42 mutant stage 13 embryos. The founder cell migration defects are also partially rescued, however some cells acquire abnormal shapes, adhere to positions more distant from the TVM both dorsally and ventrally, and form clusters, resulting in missing LVM cells in the most anterior trunk segments.

Expression of htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4twi.PG is able to induce bilaterally symmetrical flattening of the mesodermal tube onto the ectoderm in the early stages of mesoderm morphogenesis in htlAB42 embryos. Expression of htlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4twi.PG efficiently rescues the ability of mesodermal cells to move away from the site of invagination in htlAB42 embryos.

htlScer\UAS.T:λ\cI-DD ; Scer\GAL4twi.PB rescues the migration of the mesoderm after invagination in htlAB42 embryos, but only partially rescues differentiation of the mesoderm. An average of 12.2 (s.d.=2.9; n=27) hemisegments per embryo have eve expressing mesodermal cells, compared to 22 in wild-type.

Partially rescues the mesoderm migration defects of htlAB42 embryos, when expressed under the control of Scer\GAL4twi.PG.

Partially rescues the mesodermal phenotype of htlAB42 embryos when expressed under the control of Scer\GAL4twi.PG.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Encodes an activated form of htl.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
htl::λ\cIUAS.cMa
htlScer\UAS.T:l\cI-DD
htlScer\UAS.T:λ\cI-DD
htlScer\UAS.T:λ\cI
htlλ.UAS
Name Synonyms
Secondary FlyBase IDs
    References (34)