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General Information
Symbol
Dmel\styUAS.cHa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Hacohen
FlyBase ID
FBal0086309
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-spry, UAS-sty
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 2.2kb sty cDNA fragment containing the entire coding region is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Scer\GAL4repo.PL-mediated expression of styScer\UAS.cHa results in impaired glial cell wrapping in the optic stalk in larval eye discs.

Expression of styScer\UAS.cHa driven by Scer\GAL4how-24B significantly reduces the number of boutons per muscle at the neuromuscular junction of third instar larvae, compared to controls.

Expression of styScer\UAS.cHa under the control of Scer\GAL4sev.EP results in a rough-eye phenotype.

Expression of styScer\UAS.cHa under the control of Scer\GAL4hs.2sev causes misrotation and disorganization of ommatidia, missing photoreceptors in 5% of ommatidia, and external roughening of the eye.

Expression of styScer\UAS.cHa under the control of Scer\GAL41151 leads to a decrease in the number of founder cells; there are ~7 dorsal founders and ~8 lateral founders per hemisegment in Scer\GAL41151>styScer\UAS.cHa pupae, compared to 17-22 and 20 of these founder types in control pupae. However, there is no comparable change in the number of fusion-competent myoblasts.

When expression is driven by Scer\GAL4GMR.PF eyes are small and rough.

Expression of styScer\UAS.cHa under the control of Scer\GAL4dpp.blk1 has no effect on furrow initiation in the eye disc.

When expression is driven by Scer\GAL4hs.2sev or Scer\GAL4elav-C155 the eyes of the resulting flies are small and disorganized. Majority of ommatidia lack one or more outer photoreceptor cells of the R3/R4/R1/R6 type. The R7 is missing in 11+-4% of Scer\GAL4hs.2sev-driven ommatidia and 18+-6% of Scer\GAL4elav-C155-driven ommatidia. R2/R5 and R8 are unaffected. When expression is driven by Scer\GAL4en-e16E, the number of chordotonal organs in the embryo is reduced. When expression is driven by Scer\GAL4BH1, wing veins fail to form, and the size of the wing blade is reduced. This phenotype is similar to that caused by loss of function Egfr or vn mutants. When expression is driven by Scer\GAL4CY2, resulting eggs had a ventralized egg shell and the resulting embryos were also ventralized. This phenotype is similar to that caused by Egfr loss of function mutants.

When expression is driven by Scer\GAL4mat.αTub67C.T:Hsim\VP16 the resulting phenotype resembles that caused by loss of function alleles in Egfr. When expression is driven by Scer\GAL4T155 the egg is ventralized and the dorsal appendages are fused and reduced. When expression is driven by Scer\GAL4dpp.blk1 the L3 vein of the adult wing is truncated. When expression is driven by Scer\GAL4Bx-MS1096 the L2, L3 and L4 veins of the adult wing are truncated. The size of the wing is somewhat reduced.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference
Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of rauGD14083 further impairs the glial cell wrapping phenotype of Scer\GAL4repo.PL styScer\UAS.cHa mutants.

The rough-eye phenotype observed in flies expressing styScer\UAS.cHa under the control of Scer\GAL4sev.EP is suppressed in the presence of Fas2EB112/+.

When expression is driven by Scer\GAL4Bx-MS1096 in combination with btl::EgfrScer\UAS.T:λ\cI-DD, the wing size of the btl::EgfrScer\UAS.T:λ\cI-DD is largely normal and the wing veins, though still slightly thickened compared to wild type, are largely restored. When expression is driven by Scer\GAL4Bx-MS1096 in combination with htlScer\UAS.T:λ\cI-DD, the wing vein defects caused by htlScer\UAS.T:λ\cI-DD are largely suppressed. Lethality of phlScer\UAS.F179, Scer\GAL471B is rescued by styScer\UAS.cHa. Wings of phlScer\UAS.F179, styScer\UAS.cHa, Scer\GAL471B mutants are largely devoid of wing vein.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
styScer\UAS.cHa
styUAS.cHa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Hacohen
Secondary FlyBase IDs
    References (12)