FlyBase Allele Report: Dmel\Pez[1]

General Information

Symbol

Dmel\Pez¹

Species

D. melanogaster

Name

FlyBase ID

FBal0267638

Feature type

allele

Associated gene

Dmel\Pez

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Poernbacher et al., 2012)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q11term.

(Poernbacher et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:6,338,112..6,338,112 [-]

Nucleotide change:

C6338112T

Amino acid change:

Q11term | Pez-PA; Q11term | Pez-PB

Reported amino acid change:

Q?term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. The position of the mutation was deduced from Figure 1, It was shown as a CAG to TAG nonsense mutation in the small first coding exon and there is only one Gln in the exon.

(Poernbacher et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with Pez²)

(Poernbacher et al., 2012)

decreased body size

(Poernbacher et al., 2012)

decreased body size (with Pez²)

(Poernbacher et al., 2012)

hyperplasia | adult stage (with Pez²)

(Poernbacher et al., 2012)

increased occurrence of cell division | adult stage (with Pez²)

(Poernbacher et al., 2012)

semi-sterile (with Pez²)

(Poernbacher et al., 2012)

viable

(Poernbacher et al., 2012)

viable (with Pez²)

(Poernbacher et al., 2012)

Phenotype Manifest In

adult midgut epithelium (with Pez²)

(Poernbacher et al., 2012)

Detailed Description

Statement

Reference

Pez¹/Pez² adults show hyperplasia and extensive multilayering of the adult midgut epithelium. This phenotype is not seen in the larval midgut epithelium, and other larval and adult epithelia appear normal.

(Poernbacher et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pez²/Pez¹ has decreased body size phenotype, suppressible by yki[+]/yki^B5

(Poernbacher et al., 2012)

Pez²/Pez¹ has decreased body size phenotype, suppressible by Scer\GAL4^NP0001/yki^GD11187

(Poernbacher et al., 2012)

Pez²/Pez¹ has increased occurrence of cell division | adult stage phenotype, suppressible by Scer\GAL4^NP0001/yki^GD11187

(Poernbacher et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pez²/Pez¹ has adult midgut epithelium phenotype, suppressible by Scer\GAL4^NP0001/yki^GD11187

(Poernbacher et al., 2012)

Pez²/Pez¹ has adult midgut epithelium phenotype, suppressible | heat sensitive | partially by Scer\GAL4^NP0001/yki^GD11187/Scer\GAL80^ts.αTub84B

(Poernbacher et al., 2012)

Pez²/Pez¹ has adult midgut epithelium phenotype, suppressible | heat sensitive | partially by Scer\GAL4^NP0001/hpo^UAS.cWa/Scer\GAL80^ts.αTub84B

(Poernbacher et al., 2012)

Pez²/Pez¹ has adult midgut epithelium phenotype, suppressible | heat sensitive | partially by hpo^UAS.cWa/Scer\GAL4^{Delta-05151-G}/Scer\GAL80^ts.αTub84B

(Poernbacher et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of yki^GD11187 under the control of Scer\GAL4^{Myo31DF-NP0001} suppresses the increased number of mitotic cells seen in the midgut of Pez¹/Pez² adults and restores the body size of Pez¹/Pez² adults to normal.

Expression of yki^GD11187 under the control of Scer\GAL4^{Myo31DF-NP0001} during the adult stage only (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit the stage of expression) partially suppresses the increased number of mitotic cells seen in the midgut of Pez¹/Pez² adults.

Expression of hpo^Scer\UAS.cWa under the control of either Scer\GAL4^Dl-05151-G or Scer\GAL4^{Myo31DF-NP0001} during the adult stage only (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit the stage of expression) partially suppresses the increased number of mitotic cells seen in the midgut of Pez¹/Pez² adults.

Expression of yki^GD11187 under the control of Scer\GAL4^{Myo31DF-NP0001} during the adult stage only (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit the stage of expression) has no clear effect on the increased number of mitotic cells seen in the midgut of Pez¹/Pez² adults.

(Poernbacher et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Pez²/Pez¹ is rescued by Scer\GAL4^NP0001/Pez^UAS.cPa

(Poernbacher et al., 2012)

Pez²/Pez¹ is rescued by Scer\GAL4^NP0001/Pez^ΔFERM.UAS

(Poernbacher et al., 2012)

Pez²/Pez¹ is rescued by Scer\GAL4^NP0001/Pez^PD.UAS

(Poernbacher et al., 2012)

Partially rescued by

Pez²/Pez¹ is partially rescued by Pez^UAS.cPa

(Poernbacher et al., 2012)

Pez²/Pez¹ is partially rescued by Pez^UAS.cPa/Scer\GAL4^{Delta-05151-G}/Scer\GAL80^ts.αTub84B

(Poernbacher et al., 2012)

Comments

Pez^T:Avic\GFP rescues the hyperplasia of the midgut epithelium which is seen in Pez¹/Pez² adults.

In the absence of a Scer\GAL4 driver, Pez^Scer\UAS.cPa rescues the size defect of Pez¹/Pez² adults but not their midgut phenotypes.

Expression of Pez^Scer\UAS.cPa under the control of Scer\GAL4^{Myo31DF-NP0001} rescues the excess number of mitotic cells seen in the midgut of Pez¹/Pez² adults.

Expression of Pez^Scer\UAS.cPa under the control of Scer\GAL4^Dl-05151-G during the adult stage only (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit the stage of expression) significantly suppresses the increased number of mitotic cells seen in the midgut of Pez¹/Pez² adults.

Expression of Pez^{ΔFERM.Scer\UAS} or Pez^PD.Scer\UAS under the control of Scer\GAL4^{Myo31DF-NP0001} rescues the excess number of mitotic cells seen in the midgut of Pez¹/Pez² adults.

(Poernbacher et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Pez¹

(Wang et al., 2015, Poernbacher et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (2)

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