FlyBase Allele Report: Dmel\hpo[UAS.cWa]

General Information

Symbol

Dmel\hpo^UAS.cWa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct of Wu

FlyBase ID

FBal0151840

Feature type

allele

Associated gene

Dmel\hpo

Associated Insertion(s)

Carried in Construct

P{UAS-hpo.W}

Also Known As

UAS-hpo

Key Links

Transgenic product class

cDNA

(Wu et al., 2003)

wild_type

(Wu et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Wu et al., 2003)

wild_type

(Wu et al., 2003)

Mutagen

in vitro construct

(Wu et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-hpo.W}

Cytology

Description

Expression of full length hpo cDNA (GH10354), is under the control of UAS sequences.

(Wu et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

hpo

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^Bx-MS1096

(Koontz et al., 2013)

increased cell death | larval stage, with Scer\GAL4^Bx-MS1096

(Koontz et al., 2013)

lethal, with Scer\GAL4^GMR.PF

(Huang et al., 2005)

Phenotype Manifest In

NMJ bouton | increased number, with Scer\GAL4^RapGAP1-OK6

(Sakuma et al., 2016)

wing, with Scer\GAL4^Bx-MS1096

(Koontz et al., 2013, Wu et al., 2003)

wing disc, with Scer\GAL4^Bx-MS1096

(Koontz et al., 2013)

Detailed Description

Statement

Reference

Expression of hpo^Scer\UAS.cWa under the control of Scer\GAL4^RapGAP1-OK6 results in a significantly increased number of satellite boutons on neuromuscular junctions in third instar larvae.

(Sakuma et al., 2016)

Expression of hpo^Scer\UAS.cWa under the control of Scer\GAL4^C587 has no apparent germarium phenotype.

(Li et al., 2015)

Expression of hpo^Scer\UAS.cWa under the control of Scer\GAL4^Bx-MS1096 results in increased wing disc cell death and a reduction in adult wing size.

(Koontz et al., 2013)

hpo^Scer\UAS.cWa; Scer\GAL4^Bx-MS1096 animals have dramatically reduced wings.

(Wu et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^C587, ci^{RNAi.UASp.cLa}, hpo^UAS.cWa has neoplasia | female | adult stage phenotype, suppressible | partially by wts^RNAi.UAS.cUa/Scer\GAL4^C587

(Li et al., 2015)

Scer\GAL4^C587, ci^{RNAi.UASp.cLa}, hpo^UAS.cWa has neoplasia | female | adult stage phenotype, suppressible | partially by yki^UAS.cHa/Scer\GAL4^C587

(Li et al., 2015)

Scer\GAL4^Bx-MS1096, hpo^UAS.cWa has increased cell death | larval stage phenotype, suppressible | somatic clone by Tgi^ΔP

(Koontz et al., 2013)

Scer\GAL4^Bx-MS1096, hpo^UAS.cWa has abnormal size | adult stage phenotype, suppressible | partially | somatic clone by Tgi^ΔP

(Koontz et al., 2013)

Scer\GAL4^Bx-MS1096, hpo^UAS.cWa has increased cell death | larval stage phenotype, suppressible by Scer\GAL4^nub.PU/Tgi^GD7081

(Koontz et al., 2013)

Scer\GAL4^Bx-MS1096, hpo^UAS.cWa has abnormal size | adult stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/Tgi^GD7081

(Koontz et al., 2013)

Scer\GAL4^GMR.PF, hpo^UAS.cWa has lethal phenotype, suppressible by yki^UAS.cHa/Scer\GAL4^GMR.PF

(Huang et al., 2005)

Scer\GAL4^GMR.PF, hpo^UAS.cWa has lethal phenotype, suppressible | partially by Hsap\YAP1^UAS.cHa, Scer\GAL4^GMR.PF

(Huang et al., 2005)

Enhancer of

Statement

Reference

Scer\GAL4^C587/hpo^UAS.cWa is an enhancer of neoplasia | adult stage | female | temperature conditional phenotype of hh^AC2/hh^ts2

(Li et al., 2015)

Scer\GAL4^C587/hpo^UAS.cWa is an enhancer of neoplasia | adult stage | female phenotype of Scer\GAL4^C587, ci^{RNAi.UASp.cLa}

(Li et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^C587, ci^{RNAi.UASp.cLa}, hpo^UAS.cWa has germarium phenotype, suppressible | partially by wts^RNAi.UAS.cUa/Scer\GAL4^C587

(Li et al., 2015)

Scer\GAL4^C587, ci^{RNAi.UASp.cLa}, hpo^UAS.cWa has germarium phenotype, suppressible | partially by yki^UAS.cHa/Scer\GAL4^C587

(Li et al., 2015)

Scer\GAL4^Bx-MS1096, hpo^UAS.cWa has wing disc phenotype, suppressible | somatic clone by Scer\GAL4^nub.PU/Tgi^GD7081

(Koontz et al., 2013)

Scer\GAL4^Bx-MS1096, hpo^UAS.cWa has wing phenotype, suppressible | somatic clone by Scer\GAL4^nub.PU/Tgi^GD7081

(Koontz et al., 2013)

Scer\GAL4^Bx-MS1096, hpo^UAS.cWa has wing disc phenotype, suppressible | somatic clone by Tgi^ΔP

(Koontz et al., 2013)

Scer\GAL4^Bx-MS1096, hpo^UAS.cWa has wing phenotype, suppressible | somatic clone by Tgi^ΔP

(Koontz et al., 2013)

Enhancer of

Statement

Reference

Scer\GAL4^C587/hpo^UAS.cWa is an enhancer of germarium | temperature conditional phenotype of hh^AC2/hh^ts2

(Li et al., 2015)

Scer\GAL4^C587/hpo^UAS.cWa is an enhancer of germarium phenotype of Scer\GAL4^C587, ci^{RNAi.UASp.cLa}

(Li et al., 2015)

Suppressor of

Statement

Reference

Scer\GAL4^NP0001, hpo^UAS.cWa, Scer\GAL80^ts.αTub84B is a suppressor | heat sensitive | partially of adult midgut epithelium phenotype of Pez²/Pez¹

(Poernbacher et al., 2012)

hpo^UAS.cWa, Scer\GAL4^{Delta-05151-G}, Scer\GAL80^ts.αTub84B is a suppressor | heat sensitive | partially of adult midgut epithelium phenotype of Pez²/Pez¹

(Poernbacher et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The rather moderate tumorous germarium phenotype observed in hh^AC2/hh^ts2 transheterozygotes (moved to the non-permissive temperature - 29[o]C - after eclosion) or in flies expressing ci^{dsRNA.shRNA.Scer\UAS.P\T} under the control of Scer\GAL4^C587 is strongly aggravated by expression of hpo^Scer\UAS.cWa (with the Scer\GAL4^C587 driver). The enhanced phenotype of flies expressing both ci^{dsRNA.shRNA.Scer\UAS.P\T} and hpo^Scer\UAS.cWa simultaneously can in turn be partially rescued by co-expression of either wts^VDRC.cUa or yki^Scer\UAS.cHa.

(Li et al., 2015)

Tgi^ΔP mutant wing disc clones induced in flies expressing hpo^Scer\UAS.cWa under the control of Scer\GAL4^Bx-MS1096 have less apoptosis compared to when hpo^Scer\UAS.cWa is expressed alone. The reduction in adult wing size is also partially suppressed.

Expression of Tgi^GD7081 (co-expressed with Dcr-2^Scer\UAS.cDa) suppresses the wing disc apoptosis seen when hpo^Scer\UAS.cWa is expressed under the control of Scer\GAL4^nub.PU. The reduction in adult wing size is also partially suppressed.

(Koontz et al., 2013)

Expression of hpo^Scer\UAS.cWa under the control of either Scer\GAL4^Dl-05151-G or Scer\GAL4^{Myo31DF-NP0001} during the adult stage only (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit the stage of expression) partially suppresses the increased number of mitotic cells seen in the midgut of Pez¹/Pez² adults.

(Poernbacher et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

hpo^Scer\UAS.cWa

hpo^UAS.cWa

Name Synonyms

Saccharomyces cerevisiae UAS construct of Wu

(Wu et al., 2003)

Secondary FlyBase IDs

References (13)

Report Sections

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