FlyBase Allele Report: Dmel\prd1[M56]

General Information

Symbol

Dmel\prd1^M56

Species

D. melanogaster

Name

FlyBase ID

FBal0267699

Feature type

allele

Associated gene

Dmel\prd1

Associated Insertion(s)

PBac{RB3.WH3}prd1^M56

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Weiler, 2012.3.1)

Progenitor genotype

PBac{RB}prd1^e02295

(Weiler, 2012.3.1)

PBac{WH}prd1^f05458

(Weiler, 2012.3.1)

Associated Insertion(s)

PBac{RB3.WH3}prd1^M56

Cytology

Description

4161bp deletion within prd1 that deletes most of the coding region (amino acids 329-1354).

(Zong et al., 2018)

CG17360^M56 is an intragenic deletion generated from PBac{WH}CG17360^f05458 and PBac{RB}CG17360^e02295. The associated recombinant transposon insertion is PBac{RB3.WH3}CG17360^M56.

(Weiler, 2012.3.1)

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:11,781,151..11,785,312 [-]

Comment:

Scer\FRT-mediated recombination between the two progenitor insertions has resulted in the deletion of the genomic sequence between them.

Comment:

Scer\FRT-mediated recombination between the two progenitor insertions PBac{RB}prd1^e02295 and PBac{WH}prd1^f05458 has resulted in the deletion of the genomic sequence between them.

(Zong et al., 2018, Weiler, 2012.3.1)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | prepupal stage P1 | somatic clone

(Zong et al., 2018)

abnormal neuroanatomy | pupal stage P5 (with Df(3R)Exel7310)

(Zong et al., 2018)

abnormal neuroanatomy | pupal stage P5 (with Df(3R)PS2)

(Zong et al., 2018)

abnormal size | prepupal stage P1 | somatic clone

(Zong et al., 2018)

abnormal size | pupal stage P5 (with Df(3R)Exel7310)

(Zong et al., 2018)

abnormal size | pupal stage P5 (with Df(3R)PS2)

(Zong et al., 2018)

lethal - all die during pharate adult stage (with Df(3R)Exel7310)

(Zong et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

prd1^M56/Df(3R)Exel7310 and prd1^M56/Df(3R)PS2 transheterozygotes exhibit severe and highly penetrant ddaC neuron dendrite pruning defects at 16h after puparium formation, as compared to wild-type and prd1^M56 heterozygous controls.

prd1^M56/Df(3R)Exel7310 transheterozygous white prepupal ddaC neurons do not show dendritic arborization defects, whereas prd1^M56 ddaC neuron clones in white prepupae show a simplified dendritic tree (i.e. a significant decrease in dendritic termini), as compared to controls.

prd1^M56 ddaD/E neuron clones within mosaic individuals also exhibit severe dendrite pruning defects at 16h after puparium formation. ddaF neuron clones, however, are eliminated by 16h after puparium formation, similarly to controls.

(Zong et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(3R)Exel7310/prd1^M56 has abnormal neuroanatomy | pupal stage P5 phenotype, enhanceable by unc-104[+]/unc-104¹⁷⁰

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has abnormal size | pupal stage P5 phenotype, enhanceable by unc-104[+]/unc-104¹⁷⁰

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has abnormal neuroanatomy | pupal stage P5 phenotype, enhanceable by Mical¹⁵²⁵⁶/Mical[+]

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has abnormal size | pupal stage P5 phenotype, enhanceable by Mical¹⁵²⁵⁶/Mical[+]

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has abnormal neuroanatomy | pupal stage P5 phenotype, enhanceable by Cul1^EX/Cul1[+]

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has abnormal size | pupal stage P5 phenotype, enhanceable by Cul1^EX/Cul1[+]

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has abnormal neuroanatomy | pupal stage P5 phenotype, enhanceable by unc-104[+]/unc-104¹⁷²

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has abnormal size | pupal stage P5 phenotype, enhanceable by unc-104[+]/unc-104¹⁷²

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has abnormal neuroanatomy | pupal stage P5 phenotype, enhanceable by AP-2alpha[+]/AP-2α³

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has abnormal size | pupal stage P5 phenotype, enhanceable by AP-2alpha[+]/AP-2α³

(Zong et al., 2018)

Suppressed by

Statement

Reference

Df(3R)PS2/prd1^M56 has abnormal neuroanatomy | pupal stage P5 phenotype, suppressible | partially by Nrg^GL00656/Scer\GAL4^ppk.PU

(Zong et al., 2018)

Df(3R)PS2/prd1^M56 has abnormal size | pupal stage P5 phenotype, suppressible | partially by Nrg^GL00656/Scer\GAL4^ppk.PU

(Zong et al., 2018)

Enhancer of

Statement

Reference

prd1^M56/prd1[+] is an enhancer of abnormal neuroanatomy | pupal stage P5 phenotype of AP-2α^HMS00653, Scer\GAL4^ppk.PU

(Zong et al., 2018)

prd1^M56/prd1[+] is an enhancer of abnormal size | pupal stage P5 phenotype of AP-2α^HMS00653, Scer\GAL4^ppk.PU

(Zong et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(3R)Exel7310/prd1^M56 has dendritic tree | pupal stage P5 phenotype, enhanceable by AP-2alpha[+]/AP-2α³

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has larval dorsal multidendritic neuron ddaC | pupal stage P5 phenotype, enhanceable by AP-2alpha[+]/AP-2α³

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has dendritic tree | pupal stage P5 phenotype, enhanceable by Mical¹⁵²⁵⁶/Mical[+]

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has larval dorsal multidendritic neuron ddaC | pupal stage P5 phenotype, enhanceable by Mical¹⁵²⁵⁶/Mical[+]

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has dendritic tree | pupal stage P5 phenotype, enhanceable by Cul1^EX/Cul1[+]

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has larval dorsal multidendritic neuron ddaC | pupal stage P5 phenotype, enhanceable by Cul1^EX/Cul1[+]

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has dendritic tree | pupal stage P5 phenotype, enhanceable by unc-104[+]/unc-104¹⁷⁰

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has larval dorsal multidendritic neuron ddaC | pupal stage P5 phenotype, enhanceable by unc-104[+]/unc-104¹⁷⁰

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has dendritic tree | pupal stage P5 phenotype, enhanceable by unc-104[+]/unc-104¹⁷²

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 has larval dorsal multidendritic neuron ddaC | pupal stage P5 phenotype, enhanceable by unc-104[+]/unc-104¹⁷²

(Zong et al., 2018)

Suppressed by

Statement

Reference

Df(3R)PS2/prd1^M56 has dendritic tree | pupal stage P5 phenotype, suppressible | partially by Nrg^GL00656/Scer\GAL4^ppk.PU

(Zong et al., 2018)

Df(3R)PS2/prd1^M56 has larval dorsal multidendritic neuron ddaC | pupal stage P5 phenotype, suppressible | partially by Nrg^GL00656/Scer\GAL4^ppk.PU

(Zong et al., 2018)

Enhancer of

Statement

Reference

prd1^M56/prd1[+] is an enhancer of dendritic tree | pupal stage P5 phenotype of AP-2α^HMS00653, Scer\GAL4^ppk.PU

(Zong et al., 2018)

prd1^M56/prd1[+] is an enhancer of larval dorsal multidendritic neuron ddaC | pupal stage P5 phenotype of AP-2α^HMS00653, Scer\GAL4^ppk.PU

(Zong et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The pruning defects observed in prd1^M56/Df(3R)Exel7310 transheterozygous ddaC neurons at 16h after puparium formation are significantly enhanced by heterozygosity for unc-104¹⁷⁰, unc-104¹⁷² or AP-2α³, as the persisting dendrites become significantly longer.

Likewise, the ddaC neuron pruning defects induced by the Scer\GAL4^ppk.PU-driven expression of AP-2α^HMS00653 are significantly enhanced by heterozygosity for prd1^M56, as the persisting dendrites become significantly longer, but are not affected by heterozygosity for Mical¹⁵²⁵⁶ or Cul1^EX.

(Zong et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(3R)Exel7310/prd1^M56 is rescued by prd1^UAS.cZa/Scer\GAL4^ppk.PU

(Zong et al., 2018)

Df(3R)Exel7310/prd1^M56 is rescued by prd1^UAS.Venus/Scer\GAL4^ppk.PU

(Zong et al., 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

37744

w¹¹¹⁸; PBac{RB3.WH3}prd1^M56/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG17360^M56

(Zong et al., 2018, Weiler, 2012.3.1)

prd1^M56

(Zong et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (2)

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