A 703bp fragment from the Dcp-1 genome encompassing its promoter and 5'UTR is cloned upstream of a 1090bp fragment of Dcp-1 3'UTR. The Ice coding sequence, amplified from a cDNA clone, is inserted in between these two fragments.
DriceDcp-1.5'3'UTR fails to rescue DriceΔ1
The presence of the IceDcp-1.5'3'UTR construct fails to restore wild-type levels of cell death after irradiation in IceΔ1 mutants.