A 564bp fragment from the Ice genome encompassing the Ice promoter and 5'UTR is cloned upstream of a 708bp fragment of Ice 3'UTR. The Dcp-1 coding sequence, amplified from a cDNA clone, is inserted in between these two fragments.
Dcp-1Drice.5'3'UTR is rescued by Dcp-1Prev1
A Dcp-1Prev1 heterozygous or homozygous background abolishes the ability of Dcp-1Ice.5'3'UTR mutants to induce cell death.