FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\wkd220
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General Information
Symbol
Dmel\wkd220
Species
D. melanogaster
Name
FlyBase ID
FBal0268697
Feature type
allele
Associated gene
Dmel\wkd
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Ghabrial et al., 2011)
Progenitor genotype
Cytology
Description

Amino acid replacement: ?6?.

(Schottenfeld-Roames and Ghabrial, 2012)

Contains a C to T transition that results in a nonsense mutation at the sixth codon position in the wkd coding sequence.

(Schottenfeld-Roames and Ghabrial, 2012)

Nucleotide substitution: C?T.

(Schottenfeld-Roames and Ghabrial, 2012)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:11,583,365..11,583,365 [+]
Nucleotide change:

C11583365T

Reported nucleotide change:

C?T

Amino acid change:

R6term | wkd-PA; R6term | wkd-PB

Reported amino acid change:

R6term

(Schottenfeld-Roames and Ghabrial, 2012)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      wkd220/Df(3R)Exel6276 mutants exhibit overly exuberant tracheal seamless tube growth. Examination of wkd220/Df(3R)Exel6276 terminal cell tips reveals a "U-turn" phenotype in which seamless tubes execute a series of 180[o] turns. All other aspects of tracheal development appear normal at the third instar larval stage. wkd220 mutant clones in both multicellular and unicellular tubes are of normal morphology. wkd220 mutant fusion cells also appear normal.

      (Schottenfeld-Roames and Ghabrial, 2012)

      wkd220 mutants exhibit variable terminal cell pruning and defects in lumen formation, including prematurely truncated tubes that end in local dilations and discontinuous tubes.

      (Ghabrial et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Rab35S22N.Scer\UAS.T:Avic\GFP-YFP under the control of Scer\GAL4btl.PS strongly rescues the lethality and "U-turn" phenotype seen in wkd220/Df(3R)Exel6276 mutant terminal tracheal cells.

      wkd220/Df(3R)Exel6276 alters the location of the ectopic seamless tube phenotype seen in tracheal terminal cells expressing btlScer\UAS.T:λ\cI-DD under the control of Scer\GAL4btl.PS. In some cells the extra tubes are found throughout the cell (in the soma and at the branch tip), whereas in others extra tubes are present only at the branch tip. A wkd220/+ background has no effect on seamless tube localisation.

      wkd220/Df(3R)Exel6276 does not enhance the ectopic seamless tube phenotype seen in Dlic1 mutant tracheal terminal cell clones.

      wkd220/Df(3R)Exel6276 does not enhance the ectopic seamless tube phenotype seen in Dlic2 mutant tracheal terminal cells.

      Expression of Rab35S22N.Scer\UAS.T:Avic\GFP-YFP under the control of Scer\GAL4btl.PS strongly rescues the "U-turn" phenotype seen in wkd220/Df(3R)Exel6276 mutant terminal tracheal cells. The pupal stage lethality seen in wkd220/Df(3R)Exel6276 mutants is also rescued.

      (Schottenfeld-Roames and Ghabrial, 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      wkd220/Df(3R)Exel6276 is rescued by wkd+t3.9

      (Schottenfeld-Roames and Ghabrial, 2012)

      wkd220/Df(3R)Exel6276 is rescued by Scer\GAL4btl.PS/wkdUAS.mKate2

      (Schottenfeld-Roames and Ghabrial, 2012)
      Comments

      Expression of wkd+t3.9 rescues the tracheal defects and lethality seen in wkd220/Df(3R)Exel6276 mutants.

      Expression of wkdScer\UAS.T:Zzzz\eqFP578-mKate2 under the control of Scer\GAL4btl.PS rescues the tracheal terminal cell defects seen in wkd220/Df(3R)Exel6276 mutants.

      (Schottenfeld-Roames and Ghabrial, 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      220
      (Ghabrial et al., 2011)
      wkd220
      Name Synonyms
      Secondary FlyBase IDs
        References (2)