FlyBase Allele Report: Dmel\GckIII[wheezy-770]

General Information

Symbol

Dmel\GckIII^wheezy-770

Species

D. melanogaster

Name

FlyBase ID

FBal0268698

Feature type

allele

Associated gene

Dmel\GckIII

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Ghabrial et al., 2011)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q57term.

(Song et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:17,085,966..17,085,966 [+]

Nucleotide change:

C17085966T

Reported nucleotide change:

C?T

Amino acid change:

Q57term | GckIII-PA

Reported amino acid change:

Q57term

(Song et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

crossvein (with Df(3R)MAP11)

(Ghabrial et al., 2011)

embryonic/larval tracheal dorsal trunk

(Ghabrial et al., 2011)

eye | somatic clone

(Ghabrial et al., 2011)

larval tracheal system

(Ghabrial et al., 2011)

terminal tracheal cell

(Ghabrial et al., 2011)

terminal tracheal cell | third instar larval stage

(Song et al., 2013)

third instar larval cuticle

(Ghabrial et al., 2011)

Detailed Description

Statement

Reference

Homozygous GckIII^wheezy-770 mutants exhibit tracheal morphogenesis defects. The terminal cells display a transition zone tube dilation defect. No defects are seen in heterozygotes.

In GckIII^wheezy-770 mutant clones a single large dilation is detected within the tube of the transition zone.

(Song et al., 2013)

GckIII^wheezy-770 mutants exhibit mild tracheal terminal cell pruning and an air filling defect. Dorsal trunk clones show darker cuticle over the apical membrane.

GckIII^wheezy-770/Df(3R)MAP11 trans-heterozygotes exhibit a cross-veinless wing defect.

(Ghabrial et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

GckIII^wheezy-770 has terminal tracheal cell | third instar larval stage phenotype, suppressible by Scer\GAL4^btl.PS/vari^GD13798

(Song et al., 2013)

GckIII^wheezy-770 has terminal tracheal cell | third instar larval stage phenotype, suppressible by Scer\GAL4^btl.PS/crb^GD14463

(Song et al., 2013)

GckIII^wheezy-770 has terminal tracheal cell | third instar larval stage phenotype, suppressible by Nsf2^lotus-O48

(Song et al., 2013)

Enhancer of

Statement

Reference

wheezy[+]/GckIII^wheezy-770 is an enhancer of terminal tracheal cell | third instar larval stage phenotype of Ccm3^GD4767, Scer\GAL4^btl.PS

(Song et al., 2013)

NOT Suppressor of

Statement

Reference

GckIII^wheezy-770 is a non-suppressor of terminal tracheal cell | third instar larval stage phenotype of Nsf2^lotus-O48

(Song et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for GckIII^wheezy-770 enhances the penetrance of the transition zone tube dilation defect seen in third instar larval trachea expressing Ccm3^GD4767 under the control of Scer\GAL4^btl.PS.

Expression of vari^GD13798 under the control of Scer\GAL4^btl.PS partially suppresses the tracheal morphogenesis defects seen in GckIII^wheezy-770 mutant third instar larvae. Transition zone dilation is completely suppressed in 52% of terminal cells and partially suppressed in a further 20%. An additional 9% show a discontinuous tube phenotype reminiscent of that seen in Nsf2 mutants.

Expression of crb^GD14463 under the control of Scer\GAL4^btl.PS partially suppresses the tracheal morphogenesis defects seen in GckIII^wheezy-770 mutant third instar larvae. Transition zone dilation is completely suppressed in 61% of terminal cells and partially suppressed in a further 27%.

As in Nsf2^lotus-O48 mutants alone, 70% of the terminal cells in Nsf2^lotus-O48 GckIII^wheezy-770 double mutant third instar larvae show a discontinuous tube phenotype in the transition zone. However, in the remaining 30% of cells, the tube dilation seen in GckIII^wheezy-770 mutants is suppressed.

(Song et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

GckIII^wheezy-770 is rescued by GckIII^UAS.cSa/Scer\GAL4^btl.PS

(Song et al., 2013)

Comments

Pan-tracheal expression of GckIII^Scer\UAS.cSa under the control of Scer\GAL4^btl.PS rescues the tracheal morphogenesis defects seen in GckIII^wheezy-770 mutant third instar larvae.

(Song et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

63092

w^*; P{GAL4-btl.S}2, P{UASp-Act5C.T:GFP}2; P{FRT(w^hs)}2A P{neoFRT}82B GckIII^wheezy-770/TM3, P{tubP-GAL80}3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

770

(Ghabrial et al., 2011)

GckIII^wheezy-770

GckIII^wheezy770

(Poon et al., 2018)

GckIII^wheezy

(Poon et al., 2018)

wheezy⁷⁷⁰

(Song et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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