FlyBase Allele Report: Dmel\polo[UAS.N.GFP]

General Information

Symbol

Dmel\polo^UAS.N.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0268718

Feature type

allele

Associated gene

Dmel\polo

Associated Insertion(s)

Carried in Construct

P{UAS-GFP-polo.WT}

Key Links

Transgenic product class

wild_type

(Ouyang et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ouyang et al., 2011)

Mutagen

in vitro construct

(Ouyang et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-GFP-polo.WT}

Cytology

Description

UAS regulatory sequences drive expression of wild-type polo coding sequences tagged at the N-terminal with GFP.

(Ouyang et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

polo

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of polo^{Scer\UAS.N.T:Avic\GFP} in the brain, under the control of Scer\GAL4^Dll-md23, has no effect on neuroblast formation.

(Ouyang et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Dll-md23, mts^GD12133, polo^UAS.N.GFP has abnormal neuroanatomy phenotype, enhanceable by Dronc^RNAi.UAS.Ka, Scer\GAL4^Dll-md23

(Ouyang et al., 2011)

Other

Statement

Reference

Scer\GAL4^Dll-md23, mts^GD12133, polo^UAS.N.GFP has abnormal neuroanatomy phenotype

(Ouyang et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Dll-md23, mts^GD12133, polo^UAS.N.GFP has neuroblast | increased number phenotype, enhanceable by Dronc^RNAi.UAS.Ka, Scer\GAL4^Dll-md23

(Ouyang et al., 2011)

Other

Statement

Reference

Scer\GAL4^Dll-md23, mts^GD12133, polo^UAS.N.GFP has neuroblast | increased number phenotype

(Ouyang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of mts^GD12133 and polo^{Scer\UAS.N.T:Avic\GFP} in the brain, under the control of Scer\GAL4^Dll-md23, results in a 25% increase of ectopic neuroblasts. Co-expression of Nc^{dsRNA.Scer\UAS} increases this ectopic neuroblast formation by approximately 25%.

(Ouyang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

polo^{Scer\UAS.N.T:Avic\GFP}

polo^UAS.N.GFP

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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