FlyBase Allele Report: Dmel\Dronc[RNAi.UAS.Ka]

General Information

Symbol

Dmel\Dronc^RNAi.UAS.Ka

Species

D. melanogaster

Name

FlyBase ID

FBal0191075

Feature type

allele

Associated gene

Dmel\Dronc

Associated Insertion(s)

Carried in Construct

P{UAS-Dronc.RNAi.K}

Key Links

Transgenic product class

RNAi_reagent

(Kanuka et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Kanuka et al., 2005)

Mutagen

in vitro construct

(Kanuka et al., 2005, Kanuka et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Dronc.RNAi.K}

Cytology

Description

UAS sequences drive expression of a 500bp Dronc inverted repeat.

(Kanuka et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Dronc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^sca-P309

(Kanuka et al., 2005)

Phenotype Manifest In

macrochaeta | increased number, with Scer\GAL4^sca-P309

(Kanuka et al., 2005)

Detailed Description

Statement

Reference

Ectopic macrochaetae form on the scutellum in 47.3% of cases when Nc^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^sca-P309.

(Kanuka et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Dronc^RNAi.UAS.Ka, Scer\GAL4^insc-Mz1407 is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^insc-Mz1407, numb^S2D.UAS

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407/Dronc^RNAi.UAS.Ka is an enhancer of abnormal neuroanatomy phenotype of numb^S52F

(Ouyang et al., 2011)

Dronc^RNAi.UAS.Ka, Scer\GAL4^Dll-md23 is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^Dll-md23, mts^GD12133, polo^UAS.N.GFP

(Ouyang et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

Dronc^RNAi.UAS.Ka, Scer\GAL4^insc-Mz1407 is an enhancer of neuroblast phenotype of Scer\GAL4^insc-Mz1407, numb^S2D.UAS

(Ouyang et al., 2011)

Dronc^RNAi.UAS.Ka, Scer\GAL4^insc-Mz1407 is an enhancer of type II neuroblast | increased number phenotype of Scer\GAL4^insc-Mz1407, numb^S2D.UAS

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407/Dronc^RNAi.UAS.Ka is an enhancer of type II neuroblast | increased number | somatic clone phenotype of numb^S52F

(Ouyang et al., 2011)

Dronc^RNAi.UAS.Ka, Scer\GAL4^Dll-md23 is an enhancer of neuroblast | increased number phenotype of Scer\GAL4^Dll-md23, mts^GD12133, polo^UAS.N.GFP

(Ouyang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Nc^{dsRNA.Scer\UAS} in a numb^S52F background increases the number of ectopic neuroblasts.

Knockdown of Nc through expression of Nc^{dsRNA.Scer\UAS} significantly augmented numb^S2D.Scer\UAS induced ectopic neuroblast formation. This enhancement is not due to prevention of neuroblast apoptosis, as the total number of TUNEL[+] cells in the central brain doesn't change.

Co-expression of mts^GD12133 and polo^{Scer\UAS.N.T:Avic\GFP} in the brain, under the control of Scer\GAL4^Dll-md23, results in a 25% increase of ectopic neuroblasts. Co-expression of Nc^{dsRNA.Scer\UAS} in flies expressing mts^GD12133 and polo^{Scer\UAS.N.T:Avic\GFP} in the brain, under the control of Scer\GAL4^Dll-md23, increases ectopic neuroblast formation by approximately 25%.

(Ouyang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Transcription from the P{UAS-Nc.dsRNA} construct produces Nc dsRNA, resulting in dsRNA interference (RNAi) of the Nc gene.

(Kanuka et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Dronc^RNAi.UAS.Ka

Dronc^{dsRNA.Scer\UAS}

Dronc^dsRNA.UAS

Nc^{dsRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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