FlyBase Allele Report: Dmel\Lpt[RNAi.UAS]

General Information

Symbol

Dmel\Lpt^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0268747

Feature type

allele

Associated gene

Dmel\Lpt

Associated Insertion(s)

Carried in Construct

P{UAS-Lpt.RNAi}

Also Known As

cmi-IR

Key Links

Transgenic product class

RNAi_reagent

(Chauhan et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Chauhan et al., 2012)

Mutagen

in vitro construct

(Chauhan et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Lpt.RNAi}

Cytology

Description

UAS drives expression of a Lpt inverted repeat RNAi transgene.

(Chauhan et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Lpt

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^unspecified

(Chauhan et al., 2012)

partially lethal - majority die, with Scer\GAL4^unspecified

(Chauhan et al., 2012)

visible, with Scer\GAL4^C-765

(Chauhan et al., 2013)

visible, with Scer\GAL4^en-e16E

(Chauhan et al., 2013)

Phenotype Manifest In

antenna, with Scer\GAL4^ey.PU

(Chauhan et al., 2012)

eye, with Scer\GAL4^ey.PU

(Chauhan et al., 2012)

head, with Scer\GAL4^ey.PU

(Chauhan et al., 2012)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Chauhan et al., 2013)

wing vein, with Scer\GAL4^C-765

(Chauhan et al., 2012)

wing vein, with Scer\GAL4^en-e16E

(Chauhan et al., 2013)

wing vein | P-stage, with Scer\GAL4^69B

(Chauhan et al., 2013)

wing vein L2, with Scer\GAL4^C-765

(Chauhan et al., 2013)

wing vein L5, with Scer\GAL4^C-765

(Chauhan et al., 2013)

Detailed Description

Statement

Reference

Modest expression of Lpt^{dsRNA.Scer\UAS} in the wing disc under the control of Scer\GAL4^C-765 results in shortened wing veins L2 and L5 at low penetrance.

Compared with wild-type, pupal wing veins appear to be shortened in animals expressing Scer\GAL4^69B>Lpt^{dsRNA.Scer\UAS}.

Expression of Lpt^{dsRNA.Scer\UAS} in the posterior wing compartment under the control of Scer\GAL4^en-e16E results in the formation of incomplete veins only in the posterior region of the wing.

(Chauhan et al., 2013)

Widespread expression of Lpt^{dsRNA.Scer\UAS} in the presence of Dcr-2^Scer\UAS.cDa results in complete lethality, whereas knockdown without Dcr-2^Scer\UAS.cDa or knockdown in restricted patterns produces partial lethality and a broad range of developmental defects.

Scer\GAL4^69B-mediated expression of Lpt^{dsRNA.Scer\UAS} leads to insignificant changes in the number of circulating hemocytes at the 'clear gut' third instar larval stage.

Scer\GAL4^C-765-mediated expression of Lpt^{dsRNA.Scer\UAS} results in shortened wing veins that can be enhanced by co-expression of Dcr-2^Scer\UAS.cDa.

Scer\GAL4^ey.PU-mediated expression of Lpt^{dsRNA.Scer\UAS} results in reduced eye size and loss of head and antennal structures.

(Chauhan et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Lpt^RNAi.UAS, Scer\GAL4^en-e16E has visible phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^en-e16E

(Chauhan et al., 2013)

Enhancer of

Statement

Reference

Lpt^RNAi.UAS, Scer\GAL4^C-765 is an enhancer of visible phenotype of Scer\GAL4^C-765, dpp^RNAi.UAS.cUa

(Chauhan et al., 2013)

Suppressor of

Statement

Reference

Lpt^RNAi.UAS, Scer\GAL4^C-765 is a suppressor | partially of visible phenotype of Scer\GAL4^C-765, tkv^RNAi.UAS.cUa

(Chauhan et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Lpt^RNAi.UAS, Scer\GAL4^en.PU has wing blade posterior compartment phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^en.PU

(Chauhan et al., 2013)

Lpt^RNAi.UAS, Scer\GAL4^C-765 has wing vein phenotype, enhanceable by trr^GD4501, Scer\GAL4^C-765

(Chauhan et al., 2012)

Lpt^RNAi.UAS, Scer\GAL4^C-765 has wing vein phenotype, enhanceable by Utx^NIG.5640R, Scer\GAL4^C-765

(Chauhan et al., 2012)

NOT Enhanced by

Statement

Reference

Lpt^RNAi.UAS, Scer\GAL4^C-765 has wing vein phenotype, non-enhanceable by ash2^GD1374, Scer\GAL4^C-765

(Chauhan et al., 2012)

Lpt^RNAi.UAS, Scer\GAL4^C-765 has wing vein phenotype, non-enhanceable by wds^GD9379, Scer\GAL4^C-765

(Chauhan et al., 2012)

Enhancer of

Statement

Reference

Lpt^RNAi.UAS, Scer\GAL4^C-765 is an enhancer of wing vein L2 phenotype of Scer\GAL4^C-765, dpp^RNAi.UAS.cUa

(Chauhan et al., 2013)

Lpt^RNAi.UAS, Scer\GAL4^C-765 is an enhancer of wing vein L5 phenotype of Scer\GAL4^C-765, dpp^RNAi.UAS.cUa

(Chauhan et al., 2013)

Lpt^RNAi.UAS, Scer\GAL4^C-765 is an enhancer of wing vein phenotype of Scer\GAL4^C-765, trr^GD4501

(Chauhan et al., 2012)

Suppressor of

Statement

Reference

Lpt^RNAi.UAS, Scer\GAL4^C-765 is a suppressor | partially of wing phenotype of Scer\GAL4^C-765, tkv^RNAi.UAS.cUa

(Chauhan et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of dpp^{dsRNA.Scer\UAS.cUa} and Lpt^{dsRNA.Scer\UAS}, both under the control of Scer\GAL4^C-765, results in further shortening of the wing veins.

Simultaneous expression of tkv^{dsRNA.Scer\UAS.cUa} and Lpt^{dsRNA.Scer\UAS} under the control of Scer\GAL4^C-765 suppresses the tkv^{dsRNA.Scer\UAS.cUa} phenotypes.

Co-expression of Dcr-2^Scer\UAS.cDa with Lpt^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E leads to strong wing blistering.

(Chauhan et al., 2013)

Scer\GAL4^C-765-mediated co-expression of trr^GD4501 and Lpt^{dsRNA.Scer\UAS} results in further shortening of the wing veins compared to expression of either transgene alone.

Co-expression of Utx^NIG.5640R strongly enhances the wing vein shortening phenotype of Scer\GAL4^C-765, Lpt^{dsRNA.Scer\UAS} flies.

(Chauhan et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Lpt^RNAi.UAS

Lpt^{dsRNA.Scer\UAS}

Lpt^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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