FlyBase Allele Report: Dmel\trr[GD4501]

General Information

Symbol

Dmel\trr^GD4501

Species

D. melanogaster

Name

FlyBase ID

FBal0210803

Feature type

allele

Associated gene

Dmel\trr

Associated Insertion(s)

Carried in Construct

P{GD4501}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4501}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4501

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

wing vein, with Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

wing vein, with Scer\GAL4^C-765

(Chauhan et al., 2012)

Detailed Description

Statement

Reference

Scer\GAL4^69B-mediated expression of trr^GD4501 leads to insignificant changes in the number of circulating hemocytes at the 'clear gut' third instar larval stage.

Scer\GAL4^C-765-mediated expression of trr^GD4501 results in shortened longitudinal and cross veins.

(Chauhan et al., 2012)

Knockdown of trr through expression of trr^GD4501 under the control of Scer\GAL4^Bx-MS1096 causes a mild phenotype consisting of a mild loss of wing veins and mild upward curvature of the wing.

(Kleefstra et al., 2012)

Adults expressing trr^GD4501 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4501

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096, trr^GD4501 has lethal - all die before end of P-stage phenotype

(Kleefstra et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^C-765, trr^GD4501 has wing vein phenotype, enhanceable by Lpt^RNAi.UAS, Scer\GAL4^C-765

(Chauhan et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^C-765, trr^GD4501 has wing vein phenotype, non-suppressible by Lpt^UAS.Tag:HA, Scer\GAL4^C-765

(Chauhan et al., 2012)

Enhancer of

Statement

Reference

trr^GD4501, Scer\GAL4^C-765 is an enhancer of wing vein phenotype of Lpt^RNAi.UAS, Scer\GAL4^C-765

(Chauhan et al., 2012)

Suppressor of

Statement

Reference

trr^GD4501, Scer\GAL4^C-765 is a suppressor of wing vein | ectopic phenotype of Lpt^UAS.Tag:HA, Scer\GAL4^C-765

(Chauhan et al., 2012)

Other

Statement

Reference

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096, trr^GD4501 has wing disc phenotype

(Kleefstra et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^C-765-mediated co-expression of trr^GD4501 and Lpt^{dsRNA.Scer\UAS} results in further shortening of the wing veins compared to expression of either transgene alone.

Co-expression of trr^GD4501 suppresses the ectopic wing vein phenotype of Lpt^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^C-765 flies, though the trr^GD4501, Scer\GAL4^C-765 shortened wing vein phenotype persists.

(Chauhan et al., 2012)

Co-expression of trr^GD4501 with G9a^Scer\UAS.cKa overexpression (both under the control of Scer\GAL4^Bx-MS1096) results in fully penetrant pupal lethality resulting from necrosis of the entire developing wing tissue.

(Kleefstra et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v10749

w¹¹¹⁸ P{GD4501}v10749

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4501

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

trr^GD4501

(Ghezzi et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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