FlyBase Allele Report: Dmel\Lpt[UAS.Tag:HA]

General Information

Symbol

Dmel\Lpt^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0268748

Feature type

allele

Associated gene

Dmel\Lpt

Associated Insertion(s)

Carried in Construct

P{UAS-Lpt.HA}

Also Known As

HA-cmi

Key Links

Transgenic product class

cDNA

(Chauhan et al., 2012)

wild_type

(Chauhan et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Chauhan et al., 2012)

wild_type

(Chauhan et al., 2012)

Mutagen

in vitro construct

(Chauhan et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Lpt.HA}

Cytology

Description

UAS drives expression of a full-length Lpt cDNA with a Tag:HA tag at the N-terminus.

(Chauhan et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Lpt

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^unspecified

(Chauhan et al., 2012)

lethal, with Scer\GAL4^unspecified

(Chauhan et al., 2012)

visible, with Scer\GAL4^C-765

(Chauhan et al., 2013)

Phenotype Manifest In

embryonic/larval hemocyte | increased number, with Scer\GAL4^69B

(Chauhan et al., 2012)

eye, with Scer\GAL4^69B

(Chauhan et al., 2012)

sex comb | ectopic, with Scer\GAL4^69B

(Chauhan et al., 2012)

wing vein | ectopic, with Scer\GAL4^C-765

(Chauhan et al., 2013, Chauhan et al., 2012)

wing vein L2, with Scer\GAL4^C-765

(Chauhan et al., 2013)

Detailed Description

Statement

Reference

Overexpression of Lpt^{Scer\UAS.T:Ivir\HA1} in the wing disc under the control of Scer\GAL4^C-765 results in both ectopic vein formation and a disrupted proximal L2 vein.

(Chauhan et al., 2013)

Scer\GAL4^69B-mediated expression of Lpt^{Scer\UAS.T:Ivir\HA1} leads to an approximately 2-fold increase in the number of circulating hemocytes at both the 'blue gut' and the 'clear gut' third instar larval stages, with no increase in the number of lamellocytes.

Widespread, high level expression of Lpt^{Scer\UAS.T:Ivir\HA1} results in lethality at various developmental stages.

Modest Scer\GAL4^C-765-mediated expression of Lpt^{Scer\UAS.T:Ivir\HA1} results in ectopic wing veins.

Modest Scer\GAL4^69B-mediated expression of Lpt^{Scer\UAS.T:Ivir\HA1} results in eye tumours and ectopic sex combs on the distal tarsi of male prothoracic legs.

Modest Scer\GAL4^unspecified-mediated expression of Lpt^{Scer\UAS.T:Ivir\HA1} results in improper cuticle pigmentation.

(Chauhan et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lpt^UAS.Tag:HA, Scer\GAL4^C-765 has visible phenotype, suppressible | partially by dpp^RNAi.UAS.cUa, Scer\GAL4^C-765

(Chauhan et al., 2013)

Enhancer of

Statement

Reference

Lpt^UAS.Tag:HA, Scer\GAL4^C-765 is an enhancer of visible phenotype of Scer\GAL4^C-765, tkv^RNAi.UAS.cUa

(Chauhan et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lpt^UAS.Tag:HA, Scer\GAL4^C-765 has wing vein | ectopic phenotype, suppressible | partially by dpp^RNAi.UAS.cUa, Scer\GAL4^C-765

(Chauhan et al., 2013)

Lpt^UAS.Tag:HA, Scer\GAL4^C-765 has wing vein | ectopic phenotype, suppressible by usp^NIG.4380R, Scer\GAL4^C-765

(Chauhan et al., 2012)

Lpt^UAS.Tag:HA, Scer\GAL4^C-765 has wing vein | ectopic phenotype, suppressible by trr^GD4501, Scer\GAL4^C-765

(Chauhan et al., 2012)

Lpt^UAS.Tag:HA, Scer\GAL4^C-765 has wing vein | ectopic phenotype, suppressible | partially by ash2^GD1374, Scer\GAL4^C-765

(Chauhan et al., 2012)

Lpt^UAS.Tag:HA, Scer\GAL4^C-765 has wing vein | ectopic phenotype, suppressible | partially by wds^GD9379, Scer\GAL4^C-765

(Chauhan et al., 2012)

Lpt^UAS.Tag:HA, Scer\GAL4^C-765 has wing vein | ectopic phenotype, suppressible by Utx^NIG.5640R, Scer\GAL4^C-765

(Chauhan et al., 2012)

Enhancer of

Statement

Reference

Lpt^UAS.Tag:HA, Scer\GAL4^C-765 is an enhancer of wing phenotype of Scer\GAL4^C-765, tkv^RNAi.UAS.cUa

(Chauhan et al., 2013)

Lpt^UAS.Tag:HA, Scer\GAL4^C-765 is an enhancer of wing vein | ectopic phenotype of Scer\GAL4^C-765, tkv^RNAi.UAS.cUa

(Chauhan et al., 2013)

Suppressor of

Statement

Reference

Lpt^UAS.Tag:HA, Scer\GAL4^C-765 is a suppressor | partially of wing vein | ectopic phenotype of Scer\GAL4^C-765, tkv^RNAi.UAS.cUa

(Chauhan et al., 2013)

NOT Suppressor of

Statement

Reference

Lpt^UAS.Tag:HA, Scer\GAL4^C-765 is a non-suppressor of wing vein phenotype of Scer\GAL4^C-765, trr^GD4501

(Chauhan et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of Lpt^{Scer\UAS.T:Ivir\HA1} and dpp^{dsRNA.Scer\UAS.cUa} (both under the control of Scer\GAL4^C-765) results in suppression of both the incomplete vein phenotypes observed upon dpp^{dsRNA.Scer\UAS.cUa}-expression as well as the ectopic vein phenotypes observed upon Lpt^{Scer\UAS.T:Ivir\HA1} overexpression.

Co-expression of Lpt^{Scer\UAS.T:Ivir\HA1} and tkv^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^C-765 results in an enhancement of the tkv^{dsRNA.Scer\UAS.cUa} phenotypes.

(Chauhan et al., 2013)

Co-expression of usp^NIG.4380R suppresses the ectopic wing vein phenotype of Lpt^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^C-765 flies.

Co-expression of trr^GD4501 suppresses the ectopic wing vein phenotype of Lpt^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^C-765 flies, though the trr^GD4501, Scer\GAL4^C-765 shortened wing vein phenotype persists.

Co-expression of ash2^GD1374 or wds^GD9379 moderately suppresses, while co-expression of Utx^NIG.5640R strongly suppresses, the ectopic wing vein phenotype of Lpt^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^C-765 flies.

(Chauhan et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^unspecified/Lpt^UAS.Tag:HA rescues Df(2R)or-BR11/Lpt¹

(Chauhan et al., 2012)

Comments

Expression of Lpt^{Scer\UAS.T:Ivir\HA1} under Scer\GAL4 control restores viability to Lpt¹/Df(2R)or-BR11 animals.

(Chauhan et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lpt^{Scer\UAS.T:Ivir\HA1}

Lpt^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close