FlyBase Allele Report: Dmel\Lpt[1]

General Information

Symbol

Dmel\Lpt¹

Species

D. melanogaster

Name

FlyBase ID

FBal0268746

Feature type

allele

Associated gene

Dmel\Lpt

Associated Insertion(s)

Carried in Construct

Also Known As

cmi¹

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Chauhan et al., 2012)

Mutagen

Delta2-3 transposase

(Chauhan et al., 2012)

Progenitor genotype

P{EPgy2}Lpt^EY06424

(Chauhan et al., 2012)

Caused by aberration

Df(2R)Lpt¹

Cytology

Description

Excision of P{EPgy2}EY06424 resulting in a ~500bp deletion that includes the Lpt promoter and 61bp of the Lpt open reading frame. No Lpt is detectable in homozygotes.

(Chauhan et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:23,898,239..23,898,730 [+]

Comment:

Inferred boundaries of a ~500 bp deletion resulting from the imprecise excision of P{EPgy2}Lpt^EY06424, which includes the region upstream of Lpt as well as 61 bp of coding sequence.

(Chauhan et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage

(Chauhan et al., 2012)

lethal - all die before end of larval stage

(Chauhan et al., 2012)

melanotic necrosis | larval stage

(Chauhan et al., 2012)

some die during larval stage

(Chauhan et al., 2012)

Phenotype Manifest In

mouth hook

(Chauhan et al., 2012)

Detailed Description

Statement

Reference

Homozygous lethal, with the majority of mutants failing to survive beyond the second or early third larval instar stages. Many of the dying larvae retain larval mouth hooks from the L2 molt, and in some cases L2 and L3 mouth hooks are malformed, indicating a defect in cuticle shedding. Mutant larvae exhibit sluggish movement, even though they retain normal olfactory functions, touch sensitivity and phototaxis. Feeding mutant larvae with ecdysone does not affect these phenotypes.

Homozygous mutants eventually stop moving and appear to attempt pupariation. However, they fail to evert their spiracles and frequently turn brown but never produce a pupal cuticle. Many larvae develop interior necrotic patches, suggestive of larval hematopoiesis defects.

(Chauhan et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Lpt¹/Lpt[+] is an enhancer of visible phenotype of dpp^d-ho/dpp^s1

(Chauhan et al., 2013)

Lpt¹/Lpt[+] is an enhancer of visible phenotype of dpp^s1

(Chauhan et al., 2013)

Other

Statement

Reference

Lpt¹/Lpt[+], dpp^s1 has visible phenotype

(Chauhan et al., 2013)

Lpt¹/Lpt[+], dpp^d-ho/dpp^s1 has visible phenotype

(Chauhan et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

Lpt¹/Lpt[+] is an enhancer of posterior crossvein phenotype of dpp^d-ho/dpp^s1

(Chauhan et al., 2013)

Lpt¹/Lpt[+] is an enhancer of wing vein L4 phenotype of dpp^s1

(Chauhan et al., 2013)

Lpt¹/Lpt[+] is an enhancer of posterior crossvein phenotype of dpp^s1

(Chauhan et al., 2013)

Lpt¹ is an enhancer of eye phenotype of Bar¹

(Chauhan et al., 2012)

Other

Statement

Reference

Lpt¹/Lpt[+], dpp^s1 has wing vein L2 phenotype

(Chauhan et al., 2013)

Lpt¹/Lpt[+], dpp^d-ho/dpp^s1 has wing vein L2 phenotype

(Chauhan et al., 2013)

Lpt¹/Lpt[+], dpp^d-ho/dpp^s1 has posterior crossvein phenotype

(Chauhan et al., 2013)

EcR[+]/EcR^A483T, Lpt¹ has wing vein phenotype

(Chauhan et al., 2012)

Lpt¹, trr⁴ has wing vein phenotype

(Chauhan et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Approximately 4% of trans-heterozygous dpp^s1/Lpt¹ flies show a shortened posterior crossvein.

When dpp^s1 is homozygous, Lpt¹/+ flies display shortened L4, and occasionally, L2 wing veins, in addition to the enhancement of the posterior crossvein phenotype.

Approximately 6% of flies trans-heterozygous for Lpt¹/dpp^s1 dpp^d-ho display a shortened posterior crossvein.

Lpt¹/+ flies homozygous for both dpp^s1 and dpp^d-ho show enhancement of the posterior crossvein phenotype relative to trans-heterozygotes, and shortened L2 and L4 veins.

(Chauhan et al., 2013)

Flies that are heterozygous for Lpt¹ and also heterozygous for EcR^A483T, or hemizygous for trr⁴, have defects in wing patterning - most prominently, shortened veins.

Lpt¹/+ strongly enhances the reduced eye phenotype of B¹/+ flies.

(Chauhan et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(2R)or-BR11/Lpt¹ is rescued by Scer\GAL4^unspecified/Lpt^UAS.Tag:HA

(Chauhan et al., 2012)

Comments

Expression of Lpt^{Scer\UAS.T:Ivir\HA1} under Scer\GAL4 control restores viability to Lpt¹/Df(2R)or-BR11 animals.

(Chauhan et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lpt¹

cmi¹

(Chauhan et al., 2013, Chauhan et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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