FlyBase Allele Report: Dmel\Ilp8[UAS.Tag:FLAG]

General Information

Symbol

Dmel\Ilp8^UAS.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0268989

Feature type

allele

Associated gene

Dmel\Ilp8

Associated Insertion(s)

Carried in Construct

P{UAS-Ilp8.FLAG}

Also Known As

UAS-dilp8::3xFLAG, UAS-dilp8

Key Links

Transgenic product class

wild_type

(Garelli et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Garelli et al., 2012)

Mutagen

in vitro construct

(Garelli et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Ilp8.FLAG}

Cytology

Description

UAS drives expression of Ilp8 tagged with Tag:FLAG at its carboxy terminus.

(Garelli et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Ilp8

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye disc | third instar larval stage, with Scer\GAL4^αTub84B.PL

(Jaszczak et al., 2016)

Detailed Description

Statement

Reference

Ectopic expression of Ilp8^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^αTub84B.PL and in the absence of any tissue damage inhibits eye disc growth in third instar larvae and causes delay in pupariation compared to age-matched controls.

(Jaszczak et al., 2016)

Expression of Ilp8^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^αTub84B.PL or Scer\GAL4^elav.PU results in delay to pupariation, as compared to controls.

(Garelli et al., 2015)

Overexpression of Ilp8^{Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^tub.PU delays pupariation, though larvae reach the correct pupal and adult size; when mutant larvae are fed methoprene, pupae significantly larger than controls are produced, pupariation is delayed and lethality occurs.

(Vallejo et al., 2015)

Scer\GAL4^αTub84B.PL-mediated expression of Ilp8^{Scer\UAS.T:Zzzz\FLAG} delays pupariation by 55.9 +/- 7.6 hours.

Although Scer\GAL4^αTub84B.PL, Ilp8^{Scer\UAS.T:Zzzz\FLAG} larvae prolong their feeding (longer than controls), they are no larger (by volume) than controls at the time of pupariation. However, their extended feeding makes the adults weigh more than controls.

(Garelli et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ilp8^UAS.Tag:FLAG, Scer\GAL4^αTub84B.PL has abnormal size | third instar larval stage phenotype, suppressible by Lgr3^GL01056, Scer\GAL4^αTub84B.PL

(Jaszczak et al., 2016)

Ilp8^UAS.Tag:FLAG, Scer\GAL4^αTub84B.PL has abnormal developmental rate | larval stage phenotype, suppressible by Lgr3^GL01056, Scer\GAL4^αTub84B.PL

(Jaszczak et al., 2016)

Ilp8^UAS.Tag:FLAG, Scer\GAL4^αTub84B.PL has abnormal developmental rate phenotype, suppressible | partially by Lgr3^JF03217, Scer\GAL4^αTub84B.PL

(Garelli et al., 2015)

Ilp8^UAS.Tag:FLAG, Scer\GAL4^αTub84B.PL has abnormal developmental rate phenotype, suppressible by Lgr3^GL01056, Scer\GAL4^αTub84B.PL

(Garelli et al., 2015)

Ilp8^UAS.Tag:FLAG, Scer\GAL4^elav.PU has abnormal developmental rate phenotype, suppressible by Lgr3^GL01056, Scer\GAL4^elav.PU

(Garelli et al., 2015)

Ilp8^UAS.Tag:FLAG, Scer\GAL4^Tub.PU has abnormal developmental rate phenotype, suppressible by Lgr3^GL01056, Scer\GAL4^Tub.PU

(Vallejo et al., 2015)

NOT suppressed by

Statement

Reference

Ilp8^UAS.Tag:FLAG, Scer\GAL4^αTub84B.PL has abnormal developmental rate phenotype, non-suppressible by Lgr3^GL01056/Scer\GAL80^elav.PU, Scer\GAL4^αTub84B.PL

(Garelli et al., 2015)

Other

Statement

Reference

Ilp8^UAS.Tag:FLAG, Lgr3^ag1/Lgr3[+], Scer\GAL4^arm.PS has abnormal developmental rate phenotype

(Garelli et al., 2015)

Ilp8^UAS.Tag:FLAG, Lgr3^ag1, Scer\GAL4^arm.PS has abnormal developmental rate phenotype

(Garelli et al., 2015)

Ilp8^UAS.Tag:FLAG, Lgr3^ag1/Lgr3^ag8, Scer\GAL4^arm.PS has abnormal developmental rate phenotype

(Garelli et al., 2015)

Ilp8^UAS.Tag:FLAG, Lgr3^ag9/Lgr3^ag1, Scer\GAL4^arm.PS has abnormal developmental rate phenotype

(Garelli et al., 2015)

Lgr3^ag1/Df(3R)BSC321, Ilp8^UAS.Tag:FLAG, Scer\GAL4^arm.PS has abnormal developmental rate phenotype

(Garelli et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ilp8^UAS.Tag:FLAG, Scer\GAL4^αTub84B.PL has eye disc | third instar larval stage phenotype, suppressible by Lgr3^GL01056, Scer\GAL4^αTub84B.PL

(Jaszczak et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The eye disc growth inhibition as well as the pupariation delay (in the absence of tissue damage) observed in third instar larvae expressing Ilp8^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^αTub84B.PL are suppressed by co-expression of Lgr3^GL01056 (together with UAS-Dicer2 to enhance RNAi efficiency).

(Jaszczak et al., 2016)

Expression of Ilp8^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^arm.PS in a Lgr3^ag1/+ background results in delay to pupariation, as compared to controls, but this delay is partially suppressed in a Lgr3^ag1/Lgr3^ag1, Lgr3^ag1/Lgr3^ag8, Lgr3^ag1/Lgr3^ag9 or Lgr3^ag1/Df(3R)BSC321 background.

Co-expression of Lgr3^JF03217 partially suppresses and Lgr3^GL01056 fully suppresses, but co-expression of both Lgr3^GL01056 and Scer\GAL80^elav.PU fails to suppress, the developmental delay seen in flies expressing Ilp8^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^αTub84B.PL.

Co-expression of Lgr3^GL01056 suppresses the developmental delay seen in flies expressing Ilp8^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PU.

(Garelli et al., 2015)

Co-expression of Lgr3^GL01056 suppresses the delay in pupariation seen with Ilp8^{Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^tub.PU.

(Vallejo et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Ilp8^UAS.Tag:FLAG/Scer\GAL4^αTub84B.PL rescues Ilp8^ag54/Ilp8^ag50

(Heredia et al., 2021)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ilp8^{Scer\UAS.T:Zzzz\FLAG}

Ilp8^UAS.Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close