Expression of Hsap\FXN^Scer\UAS.cNa under the control of Scer\GAL4^da.G32 results in lethality before eclosion.

Ubiquitous expression of Hsap\FXN^Scer\UAS.cNa under the control of Scer\GAL4^Act.PU results in lethality.

Expression of Hsap\FXN^Scer\UAS.cNa under the control of Scer\GAL4^how-24B leads to early to late pupal lethality.

Viable progeny is produced when Hsap\FXN^Scer\UAS.cNa is expressed under the control of any one of Scer\GAL4^{neur-GAL4-A101}, Scer\GAL4^repo or Scer\GAL4^Appl.G1a. However, expression of Hsap\FXN^Scer\UAS.cNa at 29[o]C under the control of Scer\GAL4^Appl.G1a results in pre-adult lethality.

Ubiquitous expression of Hsap\FXN^Scer\UAS.cNa under the control of Scer\GAL4^unspecified results in muscular and nervous system defects in embryos at stage 16. The junctions of lateral transversal muscles 1, 2 and 3 with ventral longitudinal muscle display abnormalities compared with controls. These defects are likely due to deficient muscle growth. A few embryos exhibit abnormalities in muscular development resulting in disrupted muscular system. When compared to controls, a strong disorganization of the sensory axons in the peripheral nervous system (PNS) is observed. No abnormalities are found in the central nervous system (CNS).

Expression of Hsap\FXN^Scer\UAS.cNa in neural tissues via any one of Scer\GAL4^Appl.G1a, Scer\GAL4^{neur-GAL4-A101} or Scer\GAL4^repo results in statistically significant decline in the mean and maximum lifespans under both normal and elevated atmospheric oxygen conditions. In addition, flies expressing Hsap\FXN^Scer\UAS.cNa with any one of these drivers exhibit reduced climbing ability in an age dependent manner compared with wild-type.

Although flies expressing Hsap\FXN^Scer\UAS.cNa under the control of Scer\GAL4^Appl.G1a exhibit locomotor deficit and shortened lifespan, these flies do not display brain abnormalities compared to control age-matched individuals.

Expression of Hsap\FXN^Scer\UAS.cNa in glial cells under the control of Scer\GAL4^repo induces strong age-related degeneration in the cortex of the brain and neuropil vacuolization characterised by the presence of droplet-like structures. Ultrastructural analysis reveals complete morphological disruption of glial cells and the concomitant formation of lipid droplets. Several region of the brain exhibit clear mitochondrial phenotypes, such as abnormal morphology and vacuolization.

Hsap\FXN^UAS.cNa, Scer\GAL4^repo has short lived phenotype, non-suppressible by Cat^{Tag:Mito(Oat)}