FlyBase Allele Report: Dmel\beag[1]

General Information

Symbol

Dmel\beag¹

Species

D. melanogaster

Name

FlyBase ID

FBal0269884

Feature type

allele

Associated gene

Dmel\beag

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Beck et al., 2012)

Mutagen

ethyl methanesulfonate

(Beck et al., 2012)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?304term.

(Beck et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:9,064,083..9,064,083 [-]

Nucleotide change:

A9064083T

Amino acid change:

K303term | beag-PA

Reported amino acid change:

?304

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. The mutation was mapped to codon 303. A single point mutation at codon 304 would not result in a nonsense mutation.

(Beck et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Beck et al., 2012)

abnormal neuroanatomy | third instar larval stage (with beag^EP3260)

(Beck et al., 2012)

abnormal neuroanatomy | third instar larval stage (with Df(3R)Exel6151)

(Beck et al., 2012)

abnormal neurophysiology | third instar larval stage (with beag^EP3260)

(Beck et al., 2012)

partially lethal - majority die

(Beck et al., 2012)

Phenotype Manifest In

NMJ bouton | third instar larval stage

(Beck et al., 2012)

NMJ bouton | third instar larval stage (with beag^EP3260)

(Beck et al., 2012)

NMJ bouton | third instar larval stage (with Df(3R)Exel6151)

(Beck et al., 2012)

Detailed Description

Statement

Reference

beag¹ mutants are adult semi-lethal with 39% of the expected number surviving to adulthood.

beag¹ and beag¹/Df(3R)Exel6151 larvae have decreased numbers of NMJ synaptic boutons compared to wild type, but a significant increase in the area of boutons present, with the result that the total presynaptic area is increased compared to wild type.

beag¹/beag^EP3260 larvae have decreased numbers of NMJ synaptic boutons compared to wild type.

beag¹/+ NMJs have no change in bouton number compared to controls.

Only 6.7% if beag¹ NMJs have new boutons added to their distal ends after 48 hours, compared to 51.4% in wild type.

beag¹/beag^EP3260 animals have a 47% decrease in EJP amplitude at the larval NMJ compared with wild type. (This difference is exaggerated at high calcium concentrations.) mEJP amplitude and frequency are unchanged, leading to a 34% decrease in quantal content compared with wild type.

beag¹/Df(3R)Exel6151 NMJ terminals have no difference in the number of brp-positive active zones compared with wild type.

(Beck et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

beag¹/Df(3R)Exel6151 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/Fas2^CD8.UAS

(Beck et al., 2012)

beag¹/Df(3R)Exel6151 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/Fas2^{PEST+.AAE.UAS}

(Beck et al., 2012)

beag¹/Df(3R)Exel6151 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Fas2^UAS.cLb/Scer\GAL4^RapGAP1-OK6

(Beck et al., 2012)

NOT suppressed by

Statement

Reference

beag¹/Df(3R)Exel6151 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Smu1^UAS.cBa

(Beck et al., 2012)

beag¹/Df(3R)Exel6151 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^RapGAP1-OK6/Fas2^UAS.cLa

(Beck et al., 2012)

beag¹/Df(3R)Exel6151 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^RapGAP1-OK6/Fas2^C.UAS

(Beck et al., 2012)

Other

Statement

Reference

Fas2^e76, beag¹/beag[+] has abnormal neuroanatomy | third instar larval stage phenotype

(Beck et al., 2012)

Df(3R)Exel6182/+, beag¹ has abnormal neuroanatomy | third instar larval stage phenotype

(Beck et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

beag¹/Df(3R)Exel6151 has NMJ bouton | third instar larval stage phenotype, suppressible by Fas2^UAS.cLb/Scer\GAL4^RapGAP1-OK6

(Beck et al., 2012)

beag¹/Df(3R)Exel6151 has NMJ bouton | third instar larval stage phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/Fas2^CD8.UAS

(Beck et al., 2012)

beag¹/Df(3R)Exel6151 has NMJ bouton | third instar larval stage phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/Fas2^{PEST+.AAE.UAS}

(Beck et al., 2012)

NOT suppressed by

Statement

Reference

beag¹/Df(3R)Exel6151 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Smu1^UAS.cBa

(Beck et al., 2012)

beag¹/Df(3R)Exel6151 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^RapGAP1-OK6/Fas2^UAS.cLa

(Beck et al., 2012)

beag¹/Df(3R)Exel6151 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^RapGAP1-OK6/Fas2^C.UAS

(Beck et al., 2012)

Other

Statement

Reference

Fas2^e76, beag¹/beag[+] has NMJ bouton | third instar larval stage phenotype

(Beck et al., 2012)

Df(3R)Exel6182/+, beag¹ has NMJ bouton | third instar larval stage phenotype

(Beck et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Animals in which beag¹ is heterozygous and the Smu1 locus is hemizygous (using Df(3R)Exel6182) show an 11% decrease in bouton number compared with either single mutant.

Scer\GAL4^elav-C155-mediated overexpression of Smu1^Scer\UAS.cBa does not alter bouton number of beag¹/Df(3R)Exel6151 mutants.

Animals heterozygous for both Fas2^e76 and beag¹ have a 42% decrease in NMJ synaptic bouton number compared with wild type, whereas the single heterozygotes show no change in bouton number. Combining beag¹/Df(3R)Exel6151 with heterozygous or homozygous Fas2^e76 does not further decrease bouton number compared with beag¹/+ alone.

Scer\GAL4^Rapgap1-OK6-mediated expression of Fas2^Scer\UAS.cLb, but not Fas2^Scer\UAS.cLa or Fas2^C.Scer\UAS, rescues synaptic bouton number and average synaptic bouton area of beag¹/Df(3R)Exel6151 animals to wild type.

Scer\GAL4^Rapgap1-OK6-mediated expression of Fas2^{Scer\UAS.T:Hsap\CD8A} rescues synaptic bouton number of beag¹/Df(3R)Exel6151 animals to wild type.

Scer\GAL4^Rapgap1-OK6-mediated expression of Fas2^{PEST+.AAE.Scer\UAS} rescues synaptic bouton number of beag¹/Df(3R)Exel6151 animals to wild type.

(Beck et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

beag¹ is rescued by Scer\GAL4^elav-C155/beag^UAS.cBa

(Beck et al., 2012)

beag¹ is rescued by Scer\GAL4^VGlut1-OK371/beag^UAS.cBa

(Beck et al., 2012)

beag¹/Df(3R)Exel6151 is rescued by Scer\GAL4^RapGAP1-OK6/beag^UAS.cBa

(Beck et al., 2012)

beag¹/Df(3R)Exel6151 is rescued by Scer\GAL4^elav-C155/beag^UAS.cBa

(Beck et al., 2012)

beag¹/Df(3R)Exel6151 is rescued by Scer\GAL4^elav-C155/beag^UAS.EYFP

(Beck et al., 2012)

beag¹/Df(3R)Exel6151 is rescued by beag^Tag:FLAG

(Beck et al., 2012)

beag¹/beag^EP3260 is rescued by Scer\GAL4^RapGAP1-OK6/beag^UAS.cBa

(Beck et al., 2012)

Not rescued by

beag¹/Df(3R)Exel6151 is not rescued by Scer\GAL4^G14/beag^UAS.cBa

(Beck et al., 2012)

Comments

beag^T:Zzzz\FLAG rescues beag¹/Df(3R)Exel6151 synapse morphology defects and viability.

Expression of beag^Scer\UAS.cBa using Scer\GAL4^Rapgap1-OK6 or Scer\GAL4^elav-C155, but not Scer\GAL4^G14, fully rescues the decreased bouton number observed in beag¹/Df(3R)Exel6151 animals.

Expression of beag^{Scer\UAS.T:Avic\GFP-EYFP} using Scer\GAL4^elav-C155 fully rescues the decreased bouton number observed in in beag¹/Df(3R)Exel6151 animals.

Expression of beag^Scer\UAS.cBa using Scer\GAL4^elav-C155 or Scer\GAL4^VGlut-OK371 rescues beag¹ adult viability.

Expression of beag^Scer\UAS.cBa using Scer\GAL4^Rapgap1-OK6 fully rescues the reduction in EJP amplitude and quantal content in beag¹/beag^EP3260 mutants.

(Beck et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

beag¹

(Beck et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close