FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Fas2RNAi.UAS.A
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General Information
Symbol
Dmel\Fas2RNAi.UAS.A
Species
D. melanogaster
Name
FlyBase ID
FBal0269892
Feature type
allele
Associated gene
Dmel\Fas2
Associated Insertion(s)
Carried in Construct
P{UAS-Fas2.RNAi.A}
Key Links
Transgenic product class
RNAi_reagent
(Beck et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Beck et al., 2012)
Mutagen
in vitro construct
(Beck et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-Fas2.RNAi.A}
Cytology
Description

UAS drives expression of two copies of the Fas2 exon 12 sequence in opposite orientations, with a ftz intron sequence spacer, allowing the formation of a hairpin RNA. This RNAi construct specifically targets the transmembrane Fas2 isoforms.

(Beck et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Fas2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4da.G32-mediated expression of Fas2dsRNA.Scer\UAS.A results in a 20% decrease in synaptic bouton number compared with controls.

      (Beck et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Fas2dsRNA.Scer\UAS.A partially rescues the reduction in dendritic complexity, number of dendritic branches and total dendrite length seen in RP2 motor neurons expressing Adf1S64A.S184A.Scer\UAS.T:Zzzz\FLAG (using the Scer\GAL4eve.RRK driver to drive expression of Scer\FLP1Scer\UAS.cUa which then induces clones of cells expressing Scer\GAL4Act.PU).

      (Timmerman et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Fas2RNAi.UAS.A is rescued by Scer\GAL4da.G32/Fas2PEST+.RR.UAS

      (Beck et al., 2012)

      Fas2RNAi.UAS.A is rescued by Scer\GAL4da.G32/Fas2PEST-.RR.UAS

      (Beck et al., 2012)
      Not rescued by

      Fas2RNAi.UAS.A is not rescued by Fas2C.UAS/Scer\GAL4da.G32

      (Beck et al., 2012)
      Comments

      Scer\GAL4da.G32-mediated expression of Fas2PEST+.RNAiResistant.Scer\UAS or Fas2PEST-.RNAiResistant.Scer\UAS fully rescues the reduction in bouton number induced by Fas2dsRNA.Scer\UAS.A expression.

      Scer\GAL4da.G32-mediated expression of Fas2C.Scer\UAS does not rescue the reduction in bouton number induced by Fas2dsRNA.Scer\UAS.A expression.

      (Beck et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Fas2RNAi.UAS.A
      Fas2dsRNA.Scer\UAS.A
      Fas2dsRNA.UAS.A
      Name Synonyms
      Secondary FlyBase IDs
        References (4)