FlyBase Allele Report: Dmel\Wdr62[NIG.7337R]

General Information

Symbol

Dmel\Wdr62^NIG.7337R

Species

D. melanogaster

Name

FlyBase ID

FBal0275530

Feature type

allele

Associated gene

Dmel\Wdr62

Associated Insertion(s)

Carried in Construct

P{NIG.7337R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.7337R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-7337R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of primary autosomal recessive microcephaly 2 with or without cortical malformations

CEA

(Lim et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of primary autosomal recessive microcephaly 2 with or without cortical malformations

is exacerbated by aurA^KK100143

(Lim et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^repo

(Lim et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Lim et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^repo

(Lim et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^wor.PA

(Lim et al., 2017)

abnormal size | third instar larval stage, with Scer\GAL4^repo

(Lim et al., 2017)

decreased cell number | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Lim et al., 2017)

decreased cell number | third instar larval stage, with Scer\GAL4^repo

(Lim et al., 2017)

increased cell death | third instar larval stage, with Scer\GAL4^repo

(Lim et al., 2017)

increased occurrence of cell division | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Lim et al., 2017)

increased occurrence of cell division | third instar larval stage, with Scer\GAL4^repo

(Lim et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Wdr62^NIG.7337R under the control of Scer\GAL4^insc-Mz1407 leads to a significant decrease in the number of neuroblasts in third instar brains, a significant increase in the mitotic index of neuroblasts, and a significant decrease in the number of neuroblasts in S phase, but does not significantly alter neuroblast apoptosis levels or brain volume as compared to controls.

Expression of Wdr62^NIG.7337R under the control of Scer\GAL4^wor.PA leads to spindle misorientation in third instar neuroblasts of the brain, as compared to controls.

Expression of Wdr62^NIG.7337R under the control of Scer\GAL4^repo leads to a significant reduction in third instar brain volume, significant overall decreases in neuroblast number and glial number, and a significant increase in the mitotic index of glial and neuroblast cells, as well as a significant increase in third instar glial cell apoptosis, a significant increase in the proportion of glia in G2/M or S phase and a significant decrease in the proportion of glia in G1 phase, as compared to controls.

Expression of Wdr62^NIG.7337R under the control of Scer\GAL4^insc-Mz1407 or Scer\GAL4^repo does not significantly alter the timing of developmental stages.

Expression of Wdr62^NIG.7337R under the control of Scer\GAL4^GMR54H02 in third instar larvae does not significantly alter brain lobe volume, glial or neuroblast numbers, proportion of mitotic glia, or proportion of apoptotic glia, as compared to controls.

(Lim et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^repo, Wdr62^NIG.7337R has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by aurA^KK100143/Scer\GAL4^repo

(Lim et al., 2017)

Scer\GAL4^repo, Wdr62^NIG.7337R has abnormal size | third instar larval stage phenotype, enhanceable by aurA^KK100143/Scer\GAL4^repo

(Lim et al., 2017)

Scer\GAL4^repo, Wdr62^NIG.7337R has decreased cell number | third instar larval stage phenotype, enhanceable by aurA^KK100143/Scer\GAL4^repo

(Lim et al., 2017)

Scer\GAL4^repo, Wdr62^NIG.7337R has increased occurrence of cell division | third instar larval stage phenotype, enhanceable by aurA^KK100143/Scer\GAL4^repo

(Lim et al., 2017)

Scer\GAL4^repo, Wdr62^NIG.7337R has increased cell death | third instar larval stage phenotype, enhanceable by aurA^KK100143/Scer\GAL4^repo

(Lim et al., 2017)

Enhancer of

Statement

Reference

Wdr62^NIG.7337R/Scer\GAL4^repo is an enhancer of decreased cell number | third instar larval stage phenotype of Scer\GAL4^repo, aurA^KK100143

(Lim et al., 2017)

Wdr62^NIG.7337R/Scer\GAL4^repo is an enhancer of increased occurrence of cell division | third instar larval stage phenotype of Scer\GAL4^repo, aurA^KK100143

(Lim et al., 2017)

Wdr62^NIG.7337R/Scer\GAL4^repo is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^repo, aurA^KK100143

(Lim et al., 2017)

Wdr62^NIG.7337R/Scer\GAL4^repo is an enhancer of abnormal size | third instar larval stage phenotype of Scer\GAL4^repo, aurA^KK100143

(Lim et al., 2017)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/Wdr62^NIG.7337R is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, aurA^KK100143

(Lim et al., 2017)

Scer\GAL4^insc-Mz1407/Wdr62^NIG.7337R is a suppressor of abnormal size | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, aurA^KK100143

(Lim et al., 2017)

Scer\GAL4^insc-Mz1407/Wdr62^NIG.7337R is a suppressor of increased cell number | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, aurA^KK100143

(Lim et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/Wdr62^NIG.7337R is a non-suppressor of increased occurrence of cell division | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, aurA^KK100143

(Lim et al., 2017)

Other

Statement

Reference

Scer\GAL4^GMR54H02, Wdr62^NIG.7337R, aurA^KK100143 has increased cell death | third instar larval stage phenotype

(Lim et al., 2017)

Scer\GAL4^GMR54H02, Wdr62^NIG.7337R, aurA^KK100143 has decreased cell number | third instar larval stage phenotype

(Lim et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^repo, Wdr62^NIG.7337R has larval brain | third instar larval stage phenotype, enhanceable by aurA^KK100143/Scer\GAL4^repo

(Lim et al., 2017)

Scer\GAL4^repo, Wdr62^NIG.7337R has larval glial cell | third instar larval stage phenotype, enhanceable by aurA^KK100143/Scer\GAL4^repo

(Lim et al., 2017)

Scer\GAL4^repo, Wdr62^NIG.7337R has larval neuroblast | third instar larval stage phenotype, enhanceable by aurA^KK100143/Scer\GAL4^repo

(Lim et al., 2017)

Enhancer of

Statement

Reference

Wdr62^NIG.7337R/Scer\GAL4^repo is an enhancer of larval brain | third instar larval stage phenotype of Scer\GAL4^repo, aurA^KK100143

(Lim et al., 2017)

Wdr62^NIG.7337R/Scer\GAL4^repo is an enhancer of larval neuroblast | third instar larval stage phenotype of Scer\GAL4^repo, aurA^KK100143

(Lim et al., 2017)

Wdr62^NIG.7337R/Scer\GAL4^repo is an enhancer of larval glial cell | third instar larval stage phenotype of Scer\GAL4^repo, aurA^KK100143

(Lim et al., 2017)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/Wdr62^NIG.7337R is a suppressor of larval brain | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, aurA^KK100143

(Lim et al., 2017)

Scer\GAL4^insc-Mz1407/Wdr62^NIG.7337R is a suppressor | partially of larval neuroblast | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, aurA^KK100143

(Lim et al., 2017)

Other

Statement

Reference

Scer\GAL4^GMR54H02, Wdr62^NIG.7337R, aurA^KK100143 has larval neuroblast | third instar larval stage phenotype

(Lim et al., 2017)

Scer\GAL4^GMR54H02, Wdr62^NIG.7337R, aurA^KK100143 has larval glial cell | third instar larval stage phenotype

(Lim et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of aurA^KK100143 and Wdr62^NIG.7337R under the control of Scer\GAL4^repo in third instar larvae leads to significant enhancement of the decreased brain volume, the decreased glial and neuroblast numbers, and of the increased mitotic index of glia and neuroblasts, as compared to expression of either aurA^KK100143 or Wdr62^NIG.7337R alone.

Co-expression of aurA^KK100143 enhances the increased apoptosis of glial cells in third instar larvae expressing Wdr62^NIG.7337R under the control of Scer\GAL4^repo.

Co-expression of aurA^KK100143 and Wdr62^NIG.7337R under the control of Scer\GAL4^GMR54H02 in third instar larvae leads to a significant decrease in the number of glia, a significant increase in the proportion of apoptotic glia, and a significant decrease in the number of neuroblasts, but no significant difference in brain volume or proportion of mitotic glia, as compared to controls.

Co-expression of Wdr62^NIG.7337R suppresses the increase in brain volume and the increased neuroblast number, but fails to suppress the increased mitotic index of neuroblasts, seen in flies expressing aurA^KK100143 under the control of Scer\GAL4^insc-Mz1407.

(Lim et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

NIG-Fly

7337R-1

P{NIG.7337R}1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG7337^NIG.7337R

Wdr62^NIG.7337R

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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