FlyBase Allele Report: Dmel\Rap1[V12.hs2sev]

General Information

Symbol

Dmel\Rap1^V12.hs2sev

Species

D. melanogaster

Name

FlyBase ID

FBal0276782

Feature type

allele

Associated gene

Dmel\Rap1

Associated Insertion(s)

Carried in Construct

P{sev-hs-Rap1.V12}

Also Known As

sev.rap^V12

Key Links

Transgenic product class

missense_variant

(Mavromatakis and Tomlinson, 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Mavromatakis and Tomlinson, 2012)

Mutagen

in vitro construct

(Mavromatakis and Tomlinson, 2012)

Progenitor genotype

Carried in construct

P{sev-hs-Rap1.V12}

Cytology

Description

A 1.2kb restriction fragment containing a copy of the sev enhancer guides the expression of a mutated R sequence expressed from an Hsp70 promoter.

(Mavromatakis and Tomlinson, 2012)

Allele components

Component

Use(s)

Regulatory region(s)

sev

Encoded product / tool

Rap1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye

(Mavromatakis and Tomlinson, 2012)

ommatidium

(Mavromatakis and Tomlinson, 2012)

photoreceptor cell R7

(Mavromatakis and Tomlinson, 2012)

rhabdomere | increased number

(Mavromatakis and Tomlinson, 2012)

Detailed Description

Statement

Reference

R^V12.hs2sev flies exhibit rough eyes from the exterior and in tangential section show ommatidia containing supernumerary small rhabdomere cells. Two copies of Ras85D^V12.sev increases the strength of the phenotype, with an abundance of supernumerary small rhabdomere cells.

(Mavromatakis and Tomlinson, 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Rap1^V12.hs2sev has eye phenotype, suppressible by rl^10a

(Mavromatakis and Tomlinson, 2012)

Rap1^V12.hs2sev has photoreceptor cell R7 phenotype, suppressible by rl^10a

(Mavromatakis and Tomlinson, 2012)

Rap1^V12.hs2sev has ommatidium phenotype, suppressible by rl^10a

(Mavromatakis and Tomlinson, 2012)

Rap1^V12.hs2sev has rhabdomere | increased number phenotype, suppressible by rl^10a

(Mavromatakis and Tomlinson, 2012)

Rap1^V12.hs2sev has eye phenotype, suppressible by Df(2R)M41A10/+

(Mavromatakis and Tomlinson, 2012)

Rap1^V12.hs2sev has photoreceptor cell R7 phenotype, suppressible by Df(2R)M41A10/+

(Mavromatakis and Tomlinson, 2012)

Rap1^V12.hs2sev has ommatidium phenotype, suppressible by Df(2R)M41A10/+

(Mavromatakis and Tomlinson, 2012)

Rap1^V12.hs2sev has rhabdomere | increased number phenotype, suppressible by Df(2R)M41A10/+

(Mavromatakis and Tomlinson, 2012)

Suppressor of

Statement

Reference

Rap1^V12.hs2sev is a suppressor of photoreceptor cell R7 | somatic clone phenotype of sev¹⁴

(Mavromatakis and Tomlinson, 2012)

Rap1^V12.hs2sev is a suppressor of ommatidium | somatic clone phenotype of sev¹⁴

(Mavromatakis and Tomlinson, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The eye roughening and multiple R7 phenotype of R^V12.hs2sev mutants is significantly suppressed by the loss of one copy of rl (in rl^10a or Df(2R)M41A10 heterozygotes).

The presence of R^V12.hs2sev in a sev¹⁴ homozygous background suppresses the R7 photoreceptor loss seen in sev¹⁴ mutants.

Introduction of R^V12.hs2sev clones in sev¹⁴ eye tissue results in normally constructed ommatidia (containing a single R7 at their centre). The R7 cells in these ommatidia are invariably pigmented (indicating the presence of R^V12.hs2sev) and demonstrate the cell-autonomous rescue of sev¹⁴ in the R7 precursor by activated R.

(Mavromatakis and Tomlinson, 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

R^V12.hs2sev

Rap1^V12.hs2sev

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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